New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 2708664 =72 (0.910)3 (0.040) 3/274 NT 4.1% coding (91/957 nt) rseB anti‑sigma E factor, binds RseA
?NC_000913_3_pae_tpiA 2708700 = 68 (0.850)coding (55/957 nt) rseB anti‑sigma E factor, binds RseA
Rejected: Frequency below/above cutoff threshold.

TAACGCCTCGGCCACTCCCGCGTCCGGGGCGTTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/2708698‑2708664
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CAGAGAATAACAGGCTACCTGTCACTAATGACATGGCAAACCAAAGTTGCTTCATTACTGCGATTGCGTTCCTAAAGTTTGAATTCCTGGCACCTGTACAGCGGCTTGCTGGGT  >  NC_000913_3_pae_tpiA/2708700‑2708813
                                                                                                                                                     
TAACGCCTCGGCCACTCCCGCGTCCGGGGCGTTAGCAGAGAATAACAGGCTACCTGTCACTAATGACATGGCAAACCAAAGTTGCTTCATTACTGCGATTGCGTTCCTAAAGTTTGAATTCCTGGCACCTGTACAGCGGCTTGCTGGGT  >  2:597434/1‑149
    GCCTCGGCCACTCCCGCGTCCGGGGCGTTATCAGAGAATAACAGGCTACCTGTCA                                                                                            >  1:425012/1‑55
    GCCTCGGCCACTCCCGCGTCCGGGGCGTTATCAGAGAATAACAGGCTACCTGTCA                                                                                            <  2:425012/55‑1
                                                                                                                                                     
TAACGCCTCGGCCACTCCCGCGTCCGGGGCGTTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/2708698‑2708664
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CAGAGAATAACAGGCTACCTGTCACTAATGACATGGCAAACCAAAGTTGCTTCATTACTGCGATTGCGTTCCTAAAGTTTGAATTCCTGGCACCTGTACAGCGGCTTGCTGGGT  >  NC_000913_3_pae_tpiA/2708700‑2708813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.