Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,978,503	Δ776 bp	100%		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1978503	1979278	776	77 [0]	[0] 77	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1978502	0 (0.000)	71 (0.950)	54/258	NT	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_pae_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

GCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAAT  >  NC_000913_3_pae_tpiA/1979131‑1979425                                                                                                                                                     |                                                                                                                                                   agaaatgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgt                                                                                                                                                    <  1:784500‑M2/1‑1 (MQ=255)    aatgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgtata                                                                                                                                                 <  2:915254‑M2/4‑1 (MQ=255)      tgggtctttacacttatctaagatttttcataaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAg                                                                                                                                               >  2:1190863‑M2/144‑149 (MQ=255)         gtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCa                                                                                                                                            <  1:1302529‑M2/9‑1 (MQ=255)            tttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATAc                                                                                                                                         >  1:1209145‑M2/138‑149 (MQ=255)                     atctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgtttt                                                                                                                                <  2:1164960‑M2/21‑1 (MQ=255)                      tctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacagaaaacactccattacttaacataaatgTATAAGTCATACttttgttttg                                                                                                                               >  1:988730‑M2/128‑149 (MQ=255)                      tctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgttttg                                                                                                                               >  1:291205‑M2/128‑149 (MQ=255)                      tctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgttttg                                                                                                                               >  1:223258‑M2/128‑149 (MQ=255)                        taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                                                                             >  1:375743‑M2/126‑149 (MQ=255)                        taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                                                                             >  1:1292972‑M2/126‑149 (MQ=255)                         aagatttttcagaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgt                                                                                                                            <  2:1156571‑M2/25‑1 (MQ=255)                          agatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgtg                                                                                                                           >  1:154932‑M2/124‑149 (MQ=255)                           gatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgtgt                                                                                                                          <  1:1300945‑M2/27‑1 (MQ=255)                            atttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggcggggggctgcgcttttcaataatgcgtgatgcagatcacactaacccctcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTa                                                                                                                         >  1:1102305‑M2/122‑149 (MQ=255)                                   ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt                                                                                                                  >  1:148919‑M2/115‑149 (MQ=255)                                   ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggagctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACATTTGTTTTGGGTGTATTTAAAt                                                                                                                  >  2:710917‑M2/115‑149 (MQ=255)                                   ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggagctgcgattttcaataatgcgtaatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCAAACTTTTGTTTTTGTTGTATTTCAAt                                                                                                                  >  2:157521‑M2/115‑149 (MQ=255)                                    tcaatcgacgacactttactcgtcactacacgcacatacaacggaagggggctgcgattttcaataatgcttgatgtagatcacacaaaacactcaattacttaacataaattTATAAGTCATACTTTTGTTTTTGGTGTATTTCAATc                                                                                                                 <  2:1140559‑M2/36‑1 (MQ=255)                                    taaatcgacgcaactgtactcgtcactacacgcacatgcaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATNCTTTTGTTTTGGGTGTATTTCAATc                                                                                                                 >  1:929038‑M2/114‑149 (MQ=255)                                     aaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCt                                                                                                                <  1:148508‑M2/37‑1 (MQ=255)                                            cgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaccactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCCGTTaaaa                                                                                                         >  1:1002159‑M2/106‑149 (MQ=255)                                             gcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTAGGTGTATTTCAATCTGTTaaaaa                                                                                                        <  1:1223850‑M2/45‑1 (MQ=255)                                             gaaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttatcataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTtaaaac                                                                                                        <  1:781420‑M2/45‑2 (MQ=255)                                                actgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGtt                                                                                                     >  1:642253‑M2/102‑149 (MQ=255)                                                  tgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGtttt                                                                                                   >  1:64771‑M2/100‑149 (MQ=255)                                                   gtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGttttt                                                                                                  <  1:1100194‑M2/51‑1 (MQ=255)                                                   gtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGttttt                                                                                                  <  1:602221‑M2/51‑1 (MQ=255)                                                   gtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGttttt                                                                                                  <  1:1374560‑M2/51‑1 (MQ=255)                                                   gtactcggcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGttttt                                                                                                  <  1:1083815‑M2/51‑1 (MQ=255)                                                    tactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGATTTGCGTGTATTTCAATCTGTTAAAAAGTTTTTc                                                                                                 >  2:929736‑M2/98‑149 (MQ=255)                                                     actcgtcattacacgcacatacaacggaggggggctgcgattttcaataatggtcattagtgtgttcacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcg                                                                                                <  1:332292‑M2/53‑1 (MQ=255)                                                     actcgtcactacacgcacatacaacggaggggggctgcggttttcaataatgcgtgatgcagatcacacaaaacactcaattacttgacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcg                                                                                                <  1:203452‑M2/53‑1 (MQ=255)                                                          tcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatacagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacg                                                                                           >  1:777343‑M2/92‑149 (MQ=255)                                                                cacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagca                                                                                     <  1:482297‑M2/64‑1 (MQ=255)                                                                 acgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaa                                                                                    >  1:952956‑M2/85‑149 (MQ=255)                                                                 acgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaa                                                                                    >  1:952910‑M2/85‑149 (MQ=255)                                                                     acatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAgcaagcaa                                                                                >  2:790064‑M2/81‑149 (MQ=255)                                                                       atacaacggaggggggctgcgattttcaataatgcgtgatgcagatcccccaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaa                                                                              <  1:1251585‑M2/71‑1 (MQ=255)                                                                       atacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaa                                                                              >  1:163989‑M2/79‑149 (MQ=255)                                                                       atacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacacgcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTACAATATGTTAAAAAGTTTTTCGCTACGCTGGCAAGCaaaa                                                                              >  2:6721‑M2/79‑149 (MQ=255)                                                                       atacaacggaggggggctgcgattttcaataatgcggtatgcagaccacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaa                                                                              <  1:1251513‑M2/71‑1 (MQ=255)                                                                        tacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACTCGATCAATCaaaaa                                                                             >  2:978999‑M2/78‑149 (MQ=255)                                                                        tacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaaa                                                                             >  1:36636‑M2/78‑149 (MQ=255)                                                                           aacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGa                                                                          >  1:703703‑M2/75‑149 (MQ=255)                                                                           aacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGa                                                                          <  2:1215076‑M2/75‑1 (MQ=255)                                                                             cggaggggggtcgctattttcaataatgcgtgatgcaaatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaaa                                                                        <  1:662121‑M2/77‑1 (MQ=255)                                                                              ggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACTGTAGCAAGCAAAAATGAAAc                                                                       >  2:642268‑M2/72‑149 (MQ=255)                                                                                aggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAg                                                                     <  2:951179‑M2/80‑1 (MQ=255)                                                                                  gggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGATACGCTATCAATCAAAAATTAAACAAGa                                                                   >  2:105707‑M2/68‑149 (MQ=255)                                                                                   ggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaa                                                                  <  1:587727‑M2/83‑1 (MQ=255)                                                                                          cgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGa                                                           >  1:1053384‑M2/60‑149 (MQ=255)                                                                                             ttttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAAt                                                        >  1:434382‑M2/57‑149 (MQ=255)                                                                                                tcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATggg                                                     <  2:198227‑M2/96‑1 (MQ=255)                                                                                                 caataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACACGAATAATAGAActggta                                                    >  2:1265598‑M2/53‑147 (MQ=255)                                                                                                  aataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAACATGAAACAGGAATAATCGAAATGGGAt                                                   >  1:713857‑M2/52‑149 (MQ=255)                                                                                                  aataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGAt                                                   >  1:713452‑M2/52‑149 (MQ=255)                                                                                                    taatgcgtgatgcatctcacacaaaacactcaattacctaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGt                                                 <  2:99314‑M2/100‑1 (MQ=255)                                                                                                        gcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTgcg                                             >  2:1314892‑M2/46‑149 (MQ=255)                                                                                                            gatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGcaca                                         <  2:1174904‑M2/108‑1 (MQ=255)                                                                                                            gaagaagatcacacaaaactctcaattacttaaaatacatgTATTAGTCAAACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTATTCTCTACAATAGCAAGCAAAAATGAAACATGAATAATCGAAATGGGATGTTGCGcaca                                         <  2:1174996‑M2/108‑1 (MQ=255)                                                                                                                cagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCa                                     >  1:1115122‑M2/38‑149 (MQ=255)                                                                                                                   atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGTACAGTCaaaa                                  >  2:758666‑M2/35‑149 (MQ=255)                                                                                                                   atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCaaaa                                  <  1:1085515‑M2/115‑1 (MQ=255)                                                                                                                      acacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATaa                               >  2:546730‑M2/32‑149 (MQ=255)                                                                                                                        acaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACt                             >  2:974470‑M2/30‑149 (MQ=255)                                                                                                                          aaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCa                           >  1:446785‑M2/28‑149 (MQ=255)                                                                                                                             acactcaattacataacataaatgTATAAGTCATACTTTTGTTTTGGGTTTAATTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCg                        <  2:503975‑M2/125‑1 (MQ=255)                                                                                                                                 tcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTaaa                    >  1:1260570‑M2/21‑149 (MQ=255)                                                                                                                                   aattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAaata                  >  2:1399540‑M2/19‑149 (MQ=255)                                                                                                                                     ttacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAATCAAAATAACTCACGCTAaatcat                >  2:114694‑M2/17‑149 (MQ=255)                                                                                                                                      tacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAatc               <  1:790064‑M2/134‑1 (MQ=255)                                                                                                                                      tacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAatc               <  2:223258‑M2/134‑1 (MQ=255)                                                                                                                                        cttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTAAAAATAACTCAACATGAATAatcat             >  2:440177‑M2/14‑149 (MQ=255)                                                                                                                                         ttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAatcatc            >  1:1303280‑M2/13‑149 (MQ=255)                                                                                                                                                  atgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTataaa   <  2:1201881‑M2/146‑1 (MQ=255)                                                                                                                                                   tgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACAGAAAATAATAATGTGATataaat  >  2:981759‑M2/3‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAAT  >  NC_000913_3_pae_tpiA/1979131‑1979425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑