Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 629,270 C→T 5.3% V240V (GTC→GTT)  entA → 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA629,2700CT 5.3% 219.3 / 2.5 78V240V (GTC→GTT) entA2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (38/35);  new base T (2/2);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01

CCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGTATACCCGTCTGGGCGATGATGTGCTGGAAGCCGAAA  >  NC_000913_3_pae_tpiA/629122‑629417
                                                                                                                                                    |                                                                                                                                                   
ccGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGGCCTCGCCAGCCATATTACCCTACAGGATATTGTGGTc                                                                                                                                                     >  1:436332/1‑149 (MQ=255)
 cGAAGAACAGCGTATTCGCGGCCTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGCAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCg                                                                                                                                                    >  1:727830/1‑149 (MQ=255)
  gaagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGCTCTTGTGGTCGa                                                                                                                                                   >  1:1167279/1‑149 (MQ=255)
  gaagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGa                                                                                                                                                   >  1:1119442/1‑149 (MQ=255)
  gaagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCGCGCCAGCCATATTACCCTACAGGATATTGTGGTCGa                                                                                                                                                   <  2:456036/149‑1 (MQ=255)
  gaagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGACTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGa                                                                                                                                                   <  1:343920/149‑1 (MQ=255)
   aagaaAAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACCATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAt                                                                                                                                                  <  2:104835/149‑1 (MQ=255)
     gaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCCGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCGAGCCATATTACCCTACAGGAGATTGTGGTCGATgg                                                                                                                                                >  1:1057810/1‑149 (MQ=255)
        cAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATggcgg                                                                                                                                             >  1:460643/1‑149 (MQ=255)
              aTTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAAc                                                                                                                                       <  1:1298586/149‑1 (MQ=255)
                 cgcgGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCGGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGA‑TTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTg                                                                                                                                   <  1:33142/149‑1 (MQ=255)
                  gcgGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTg                                                                                                                                   >  2:656877/1‑149 (MQ=255)
                  gcgGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTGTTTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCCACGCTg                                                                                                                                   >  2:191/1‑149 (MQ=255)
                     gCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCGGCCATATTACCCTACAGGATCTTGTGTTCCGAGGCGGCTCACAGCTgggg                                                                                                                                >  1:29645/1‑149 (MQ=255)
                       tttGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGc                                                                                                                              >  1:559134/1‑149 (MQ=255)
                         tGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGACAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCTCCTCTGACCTCGCCAGCCATAATACCCTCCCGGCAATTGTGGACGATGGCGGCTAAAGGGCTGGGGCAt                                                                                                                            >  2:1235849/1‑149 (MQ=255)
                              aGCAGTTTAAAGTCGGAATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCa                                                                                                                       <  1:171990/149‑1 (MQ=255)
                              aGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGCGATCGCCAACACGCTTTTGTTCATCGCCTCTGACCTCGCCAGCCATATTANCCTACCGGATATTGTGGTCGATGNCGGCTCANCGCTGGGGGCGTAAGCa                                                                                                                       >  1:909815/1‑149 (MQ=255)
                               gCAGTTTAACGCCGGTATTCCGCGGGGGAAAACCGCCCGTCCATCAGAGATCGCCCACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCAt                                                                                                                      <  2:10717/149‑1 (MQ=255)
                               gCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATGGCCAGCACGTTTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATCGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCAt                                                                                                                      >  1:1341103/1‑149 (MQ=255)
                                   tttAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATc                                                                                                                  <  1:551895/149‑1 (MQ=255)
                                   tttAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATc                                                                                                                  >  1:168817/1‑149 (MQ=255)
                                   tttAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATc                                                                                                                  <  1:379097/149‑1 (MQ=255)
                                   tttAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCCCAACGCTGGGGGCATAAGCATGATc                                                                                                                  >  1:575444/1‑149 (MQ=255)
                                         ctgggCAGTCCGCTGGGGAATATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCTTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGaaa                                                                                                            <  2:1027057/146‑1 (MQ=255)
                                          tgggaagtCCGCTGGGGACAGTCGCCCGTCCACAAGAGATTGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAAc                                                                                                           <  2:1249612/142‑1 (MQ=255)
                                          tCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTGAGCTCTGGAGGCAAAAGCATGACCTGGAAAc                                                                                                           >  2:72696/1‑149 (MQ=255)
                                           cGGCATTCCGCTGGGGAGAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGTGCATAAGCATGATCTGGAAACg                                                                                                          <  1:1062586/149‑1 (MQ=255)
                                                 tCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTTGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCAttt                                                                                                    >  2:331332/1‑149 (MQ=255)
                                                 tCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTTGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCAttt                                                                                                    >  2:1032007/1‑149 (MQ=255)
                                                   cgtcggggAAATTCGCCCGTCCACAAGCGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTaa                                                                                                  <  2:293255/145‑1 (MQ=255)
                                                    gCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAAc                                                                                                 >  2:491474/1‑149 (MQ=255)
                                                       ggggAAAATCGCCCGTCCACAAGAGATCGCCAACACGCTTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCt                                                                                              <  2:732410/149‑1 (MQ=255)
                                                       ggggAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCt                                                                                              <  2:746769/149‑1 (MQ=255)
                                                          gAAAATCGCCCGTCCACAAGAGATCGCCGACACGATTTTGTTCCTCGCCTGTGACCTCGCCAGCCATATTGCCCTACAGGATATGGTGGTGGATGGCGGCTCAACGCTGGGGGCATAAGCATGGTCTGCAAAGGGCGTTTGACGCtggc                                                                                           >  1:2619/1‑146 (MQ=255)
                                                          gAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcga                                                                                           <  1:1424573/149‑1 (MQ=255)
                                                                 gCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTg                                                                                    <  1:656877/149‑1 (MQ=255)
                                                                    cGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTCAAGGAGATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAAc                                                                                 >  1:911338/1‑149 (MQ=255)
                                                                       ccACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGATGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGACCGcc                                                                              >  2:591030/1‑149 (MQ=255)
                                                                         acaAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGccac                                                                            >  1:1279314/1‑149 (MQ=255)
                                                                           aaGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGccacaa                                                                          <  1:136118/149‑3 (MQ=255)
                                                                              agaTCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTAGACTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCg                                                                       >  1:1341527/1‑149 (MQ=255)
                                                                              agaTCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGCATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCg                                                                       >  1:566364/1‑149 (MQ=255)
                                                                                   gCCAACACGATTTTGTCCCTCGCCTCTGACCTCGCCAGCCATATTACCCTAAAGAATAGTGGGGGCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAac                                                                  <  1:1396832/149‑1 (MQ=255)
                                                                                    ccAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCATACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAaca                                                                 >  1:617062/1‑149 (MQ=255)
                                                                                         agcaTTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTCCCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTCGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAAtg                                                            <  2:133920/146‑1 (MQ=255)
                                                                                         acGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATAACCCCACAGGATATTGGGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATTATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAAtg                                                            <  1:485830/149‑1 (MQ=255)
                                                                                           gATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAAtggt                                                          >  1:1379105/1‑149 (MQ=255)
                                                                                             ttttGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAAtggtgg                                                        >  1:1196865/1‑149 (MQ=255)
                                                                                              tttGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGgc                                                       <  1:1351825/149‑1 (MQ=255)
                                                                                              tttGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGgc                                                       <  1:423960/149‑1 (MQ=255)
                                                                                              tttGTTCCTCGCATCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGgc                                                       <  2:711010/149‑1 (MQ=255)
                                                                                                 gTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCTATAACACAATGGTGGCGCa                                                    >  2:544173/1‑149 (MQ=255)
                                                                                                   tCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCACTTAACGCTCGACGAACTGACCGCCACCAGCGATAACACAATGGTGGCGCATc                                                  >  2:819422/1‑149 (MQ=255)
                                                                                                   tCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCACTTAACGCTCGACGAACTGAACGCCACCAACGATAACACAATGGTGGCGCAtg                                                  >  2:819629/1‑148 (MQ=255)
                                                                                                          ctctGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAAt                                           <  1:191/149‑1 (MQ=255)
                                                                                                           tctGTCCTCGCCATCCATATTACCCTACAGTATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAAtt                                          <  1:88734/149‑1 (MQ=255)
                                                                                                           tctGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAAtt                                          >  2:440023/1‑149 (MQ=255)
                                                                                                             tGACCTCGCCAGCCATATTACCCTACAGGATATTATGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAAGGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATtgt                                        >  1:131720/1‑149 (MQ=255)
                                                                                                              gACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATtgtg                                       <  1:910588/149‑1 (MQ=255)
                                                                                                              gACCTCGCCAGCCATATTACCCTACAGGATAGTGGGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATtgtg                                       <  1:32442/149‑1 (MQ=255)
                                                                                                                ccTCGCCAGCCATATTACCCTACAGGATATTGTGGTTGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGta                                     <  1:331332/149‑1 (MQ=255)
                                                                                                                ccTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGTCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGta                                     <  1:980983/149‑1 (MQ=255)
                                                                                                                ccTCGCCAGCCATATTACCCACCAGGATATTGTGGTTGATGGCGGCTCACCGTTCCCATTTTGAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGTATTGTGta                                     <  1:1032007/149‑1 (MQ=255)
                                                                                                                 cTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGGATGGACTGGAAACGCCATTTAAAGCTCGACGAACTGAACGCCACCACCGATAACACAATGGTGGCGCATCTGGGGAGTTTGtat                                    >  1:118689/1‑149 (MQ=255)
                                                                                                                     ccagccaTATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGTATAccc                                <  1:400485/149‑1 (MQ=255)
                                                                                                                       agccaTATTACCCTACAGGCTCTCGGGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGAATACCCGt                              >  2:315951/1‑149 (MQ=255)
                                                                                                                          caTATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGCATTGTGTATACCCGTCTg                           >  2:1145667/1‑149 (MQ=255)
                                                                                                                           atatTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGTATACCCGTCTgg                          >  1:239519/1‑149 (MQ=255)
                                                                                                                             atTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGTATACCCGTCTGGGc                        <  1:789161/149‑1 (MQ=255)
                                                                                                                               tACCCTACAGGATATCGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGTTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGTATACCCGTCTGGGCga                      <  1:984610/149‑1 (MQ=255)
                                                                                                                                  cctaactgATACTGTGGTCGATGGCGGGTCAACGCTGGGGGCATAAGCATGATCTGGAAACTGCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGTATACCCGTCTGGGCgatga                   <  2:29645/142‑1 (MQ=255)
                                                                                                                                     aCAGGATATTGTGGTCGATGGCTGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGTATACCCGCCTGGGCGAGGAtgt                >  1:743872/1‑149 (MQ=255)
                                                                                                                                      cAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGTATACCCGTCTGGGCGATGAtgtg               <  1:601300/149‑1 (MQ=255)
                                                                                                                                             ttgtggttgtggGCGGCTCAATGGTGGGTACATAAGCATGATCTGGAAACGCAATTTAACGCGCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGTATACCCGTCTGGGCGATGATGTGCTGGAAg        <  2:899988/149‑1 (MQ=255)
                                                                                                                                                tgGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGTATACCCGTCTGGGCGCTGATGTGCGGAAAGCCg     >  1:522768/1‑149 (MQ=255)
                                                                                                                                                 ggTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCCTCTGGGAATTGTGTATACCCGTCTGGGCGATGATGTGCTGGAAGCCGa    <  1:1267478/149‑1 (MQ=255)
                                                                                                                                                  gTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGTATACCCGTCTGGGCGATGATGTGCTGGAAGCCGaa   >  2:741403/1‑149 (MQ=255)
                                                                                                                                                  gTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGTATACCCGTCTGGGCGATGATGGGCTGGAAGCCGaa   >  1:613140/1‑149 (MQ=255)
                                                                                                                                                   tCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGTATACCCGTCTGGGCGATGATGTGCTGGAAGCCGaaa  >  2:97547/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTGGCGCATCTGGGAATTGTGTATACCCGTCTGGGCGATGATGTGCTGGAAGCCGAAA  >  NC_000913_3_pae_tpiA/629122‑629417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑