Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 898,300 C→T 5.0% A104A (GCC→GCT ybjO → DUF2593 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA898,3000CT 5.0% 230.8 / 3.0 80A104A (GCC→GCTybjODUF2593 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (39/37);  new base T (2/2);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01

ATTCATCGCAGCGTACAAACCTGGAGTTTAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCTCATCCTGATGCTGCTGTTCGTTCCCTCGA  >  NC_000913_3_pae_tpiA/898154‑898448
                                                                                                                                                  |                                                                                                                                                    
attcatCGCAGCGTACAAACCTGGAGTTTAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACcgccgc                                                                                                                                                    <  1:569885/149‑1 (MQ=255)
 ttcatCGCAGCGTACAAACCTGGAGTTTAACGCTGTTCTTTTTAAGCAGTCTGGTGCTGGTGTTCATTGAGATCTGGTGTGCGTTGTCCCTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTTATGACACAAGTAAcagccgag                                                                                                                                                   >  2:1048591/1‑147 (MQ=255)
 ttcatCGCAGCGTACAAACCTGGAGTTTAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCa                                                                                                                                                   >  2:589541/1‑149 (MQ=255)
 ttcatCGCAGCGTACAAACCTGGAGAATAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCa                                                                                                                                                   >  1:54234/1‑149 (MQ=255)
     tCGCAGCGTACAAACCTGGAGTTTAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGtt                                                                                                                                               >  2:1200436/1‑149 (MQ=255)
      tgCAGCGTACAATCCTGGAGTTTAACTCTGGTTTGTTTAAGCAGTCTTGTTCTTGTTTTCCTTGAGATCTTGTGTGCTTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTa                                                                                                                                              <  2:626033/148‑1 (MQ=255)
       gCAGCGTACAAACCTGGAGTTTAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATAGCTGCTGACACAAATCACCGCCGCAAGTTAc                                                                                                                                             >  1:1049932/1‑149 (MQ=255)
         aGCGTACAAACCTGGAGTTTAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACtt                                                                                                                                           >  2:767708/1‑149 (MQ=255)
           cGTACAAACCTGGAGTTTAACGCTGGTCTTTGTAAGCAGTCTGGTGCTGGTTTTCATTGAGCTCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTtgt                                                                                                                                         <  2:732170/149‑1 (MQ=255)
            gTACAAACCTGGAGTTTAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTtgtg                                                                                                                                        <  1:1113904/149‑1 (MQ=255)
            gTACAAACCTGGAGTTTAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTtgtg                                                                                                                                        <  2:651035/149‑1 (MQ=255)
            gTACAAACCTGGAGTTTAACGCTCGTCTTTTTAAGCAGTCTGGTGCTGTTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTtgtg                                                                                                                                        <  2:277023/149‑1 (MQ=255)
                 aaCCTGGAGTTTACCGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCACAACTGACTTGTGggcgg                                                                                                                                   <  2:883091/149‑1 (MQ=255)
                          tttAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCTGCAAGTTACTTGTGGGCGGCTTCGCTgg                                                                                                                          >  1:235003/1‑149 (MQ=255)
                          tttAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCTGCAAGTTACTTGTGGGCGGCTTCGCTgg                                                                                                                          >  1:234656/1‑149 (MQ=255)
                          tttAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCCgg                                                                                                                          >  1:321699/1‑149 (MQ=255)
                          tttAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCAGTGAGATCTGGTGTGAGTTTTCCTTCGTGGACGGGCGGCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTgg                                                                                                                          >  2:1291999/1‑149 (MQ=255)
                           ttAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTggg                                                                                                                         <  2:180935/149‑1 (MQ=255)
                           ttAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCGTCGCTggg                                                                                                                         >  1:255813/1‑149 (MQ=255)
                            tAACGCTGGTTTTTTTACGGAGGCTGGTGCTGGTTTTCATTGAGATCCGGCGTGCGCTGTCACTGGTGAACGCGCGTCGATGGGAGCGCTGGGTATATCTGCTGACGCAAATCACCGCCTCAAGTGACTTGGGGGCGGCTTCGCTgggg                                                                                                                        <  2:285253/149‑1 (MQ=255)
                               cGCTGGTCTTTTTAAGCAGTCTGGTGCGGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTaa                                                                                                                     >  1:363673/1‑148 (MQ=255)
                                gCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACg                                                                                                                    <  1:65628/149‑1 (MQ=255)
                                gCTGGTCTTTGTGAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGGGCGTTTTCACGGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACg                                                                                                                    <  2:687288/149‑1 (MQ=255)
                                 cTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACgg                                                                                                                   <  2:1409218/149‑1 (MQ=255)
                                    gTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTa                                                                                                                >  1:1377618/1‑149 (MQ=255)
                                     tCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTTAGAGGGCGTAGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTCGGCGGCTTCGATGGGGTACGGTGAt                                                                                                               >  2:237248/1‑149 (MQ=255)
                                     tCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTAt                                                                                                               >  2:626442/1‑149 (MQ=255)
                                      cTTTTTAAGCAGTCTGGTTCTGGTTNTGATCGAGATCTGGTGTGCTTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATc                                                                                                              <  1:757561/149‑1 (MQ=255)
                                       tttttAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATcc                                                                                                             >  1:1363031/1‑149 (MQ=255)
                                       tttttAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATcc                                                                                                             <  1:509714/149‑1 (MQ=255)
                                       tatttAAGCAGTCTCGTGCTGGTTTTCATTGAGATCTTGTGTGCGTTTTCAATGGTGAAAGGGCGTCGCTGGGCGCGCTGGATATATCTGCTGACAAAAATCACAGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGTGTACGGTTATcc                                                                                                             >  2:708100/3‑149 (MQ=255)
                                                 gTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTc                                                                                                   >  1:318959/1‑149 (MQ=255)
                                                  tCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCGGGAACTGTTCa                                                                                                  >  2:1098519/1‑149 (MQ=255)
                                                   cTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAg                                                                                                 >  1:517942/1‑149 (MQ=255)
                                                    tGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGc                                                                                                >  1:1324357/1‑149 (MQ=255)
                                                    tGGTGCTGGTTTTCATTGAGACCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGc                                                                                                >  2:1093825/1‑149 (MQ=255)
                                                     ggTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTccggg                                                                                               <  2:540107/149‑5 (MQ=255)
                                                        gCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTc                                                                                            >  1:846045/1‑149 (MQ=255)
                                                         cTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTcc                                                                                           >  2:342198/1‑149 (MQ=255)
                                                          tGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTccc                                                                                          >  2:1269292/1‑149 (MQ=255)
                                                           ggctttCCTGGAGGTCTGGTGTGCGTTTTCACTGGTGGAAGGGCGTCGCTGCGCGCGCTGGCTATATCTGCTGACACAAATCACCTCCGCAAGTTACTTGTGGAAAACGTGGCTTGGGTACGGTTGTCCGGAGCTGTTCAGCATTCCCg                                                                                         <  1:416440/146‑1 (MQ=255)
                                                             ttttCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGt                                                                                       >  1:499224/1‑149 (MQ=255)
                                                             ttttCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGt                                                                                       <  1:589541/149‑1 (MQ=255)
                                                               ttCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGa                                                                                     <  2:1111044/149‑1 (MQ=255)
                                                                   ttGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCCGCGCGCGCTGGCTATATCTTCTGACACAAATCGCAGCCGCAAGTTACTTGTGGGAGGCTTCGCTGGCGTACGGTTATCCGGAGCTGTTCTCCCTTGCTGCTGAATCa                                                                                 >  2:255782/1‑149 (MQ=255)
                                                                       gaTCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAAc                                                                             >  2:163452/1‑149 (MQ=255)
                                                                        aTCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTACCGCTGAATCAAAACg                                                                            >  2:545102/1‑149 (MQ=255)
                                                                         tccggTGGGCGTTTTCACTGGTGAAAGGGCGGCGGCGCGCGGCCTGGCTATAGCTGCTGACAGAAATCACCGGCGCGAGTTAGTTGTGGTTGGGCTCGCTCGCGTACAGCCCGCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGt                                                                           <  2:1225238/146‑1 (MQ=255)
                                                                         tCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGt                                                                           >  1:206381/1‑149 (MQ=255)
                                                                                tgCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCt                                                                    >  1:767458/1‑149 (MQ=255)
                                                                                 gCGTTTTCACTTGTGAAAGGGCGTCGCTGGCCACGCTGGCTATACCTGCTGACACAAATCACCGCCGCAAGTTACCTGTGTGCGGCTTCGCTTGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCtt                                                                   <  1:121909/149‑1 (MQ=255)
                                                                                  cGTTTTTACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTc                                                                  <  2:1122721/149‑1 (MQ=255)
                                                                                  cGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCTGCAAGTTACTTGTGGGCGGCTTCGCTTGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTAAATCAAAACGTGAAATCTTc                                                                  <  2:234656/149‑1 (MQ=255)
                                                                                  cGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCTGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTc                                                                  <  2:235003/149‑1 (MQ=255)
                                                                                  cGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTc                                                                  <  1:1382432/149‑1 (MQ=255)
                                                                                  cGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTc                                                                  <  2:332781/149‑1 (MQ=255)
                                                                                    ttttCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATGACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCa                                                                >  1:289020/1‑149 (MQ=255)
                                                                                    ttttCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCa                                                                >  2:380235/1‑149 (MQ=255)
                                                                                        cACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGGGAATCAAAACGTGAAATCTTCCATAGc                                                            >  1:401363/1‑149 (MQ=255)
                                                                                        cACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGAGCGGCTTCGCTGGGGGACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGc                                                            >  1:985136/1‑149 (MQ=255)
                                                                                          cTGGGGAAAGGGCGTCGCCGGGCGCGCTGGCTATACCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCt                                                          <  1:593620/149‑1 (MQ=255)
                                                                                             gTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGAt                                                       >  1:25077/1‑149 (MQ=255)
                                                                                               gAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGCATCAAAACGTGAAATCTTCCATAGCCTGAtgc                                                     >  2:296057/1‑149 (MQ=255)
                                                                                                aaaGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAACCGTGAAATCTTCCATAGCCTGAtgct                                                    <  1:812855/149‑1 (MQ=255)
                                                                                                 aaGGGCGTCGCTGGGCGCGTTGGCTATAGCCGCTGACCCAAACCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGAtgctg                                                   <  1:337165/149‑1 (MQ=255)
                                                                                                  aggtgttCGCTGGTCTTGATGGCTATATCTGCTGACACCAATCACCGCCTCATGTTACTTGTGGGCGGCTTCGCTGGTGTACGTTTATCCGGATCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGAtgctgc                                                  <  2:763782/143‑1 (MQ=255)
                                                                                                  aGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCACGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGAtgctgc                                                  <  1:1139902/149‑1 (MQ=255)
                                                                                                   gggCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTACCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCa                                                 <  2:1407678/149‑1 (MQ=255)
                                                                                                    ggCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACCTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGCGAAATCTTCCATCGCCTGATGCTGCac                                                >  2:508729/1‑148 (MQ=255)
                                                                                                      cGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGaa                                              <  1:1200436/149‑1 (MQ=255)
                                                                                                       gTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCCCCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGGGAATCAAAACGTGACATATTCCATATGCTGTAGCTGCAGAAg                                             >  2:292059/1‑149 (MQ=255)
                                                                                                        tCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGc                                            >  2:835004/1‑149 (MQ=255)
                                                                                                         cgatGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGATTTGTTCAGCATTCCCGGTGAATCAAAACGTTAAATCTTCCATAGCCTGATGCTGCAGAAGCt                                           <  1:683598/146‑1 (MQ=255)
                                                                                                            ggggCGCGGTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGcc                                        <  2:196533/148‑1 (MQ=255)
                                                                                                             gggCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCg                                       <  1:502416/149‑1 (MQ=255)
                                                                                                                             tCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCTCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCTCATCCTGa                       >  1:447788/1‑149 (MQ=255)
                                                                                                                             tCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGGACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCTCATCCTGa                       <  1:342542/149‑1 (MQ=255)
                                                                                                                             gctgctgACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCTCATCCTGa                       <  1:421325/148‑1 (MQ=255)
                                                                                                                                gctgACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCTCATCCTGAtgc                    <  1:1269292/149‑1 (MQ=255)
                                                                                                                                  tgACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGa                                                                                                          >  1:248914/1‑61 (MQ=255)
                                                                                                                                  tgACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGa                                                                                                          <  2:248914/61‑1 (MQ=255)
                                                                                                                                             aCCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCTCATCCTGATGCTGCTGTTCGTTcc       <  2:1038690/149‑1 (MQ=255)
                                                                                                                                              ccgccgCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGGGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGATCATCCTGATGCTGCTGTTCGTTccc      >  2:1296273/1‑149 (MQ=255)
                                                                                                                                                gccgcAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCTCATCCTGATGCTGCTGTTCGTTCCCTc    <  2:1324357/149‑1 (MQ=255)
                                                                                                                                                  cgcAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCTCATCCTGATGCTGCTGTTCGTTCCCTCGa  <  2:206381/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ATTCATCGCAGCGTACAAACCTGGAGTTTAACGCTGGTCTTTTTAAGCAGTCTGGTGCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCTCATCCTGATGCTGCTGTTCGTTCCCTCGA  >  NC_000913_3_pae_tpiA/898154‑898448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: