Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 2,304,522 G→T 7.4% intergenic (+129/+586) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,304,5220GT 7.4% 158.4 / 3.8 66intergenic (+129/+586)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base G (30/29);  new base T (2/3);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

ACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGC  >  NC_000913_3_pae_tpiA/2304374‑2304669
                                                                                                                                                    |                                                                                                                                                   
acgtcgcgGTAGTCCGCTAAAGTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCg                                                                                                                                                     <  2:1023058/145‑1 (MQ=255)
 cAACGCGGCAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGAGCGTCGTATCAGGCCTCCACCGCGGTGAAGTGCgg                                                                                                                                                    >  1:1325061/1‑149 (MQ=17)
          aGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCGGATGCGACGCTGGCGCGTCGCACCAGGCCTACACCGCTGCGCCAGGCCGCACCCCGTa                                                                                                                                           >  1:635770/1‑149 (MQ=14)
          aGTTCGCAAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTa                                                                                                                                           <  2:685298/149‑1 (MQ=255)
           gTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                                                                                                                                          <  1:1094384/149‑1 (MQ=255)
                cTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTa                                                                                                                                           <  2:193719/143‑1 (MQ=9)
                cTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTa                                                                                                                                           >  1:193719/1‑143 (MQ=17)
                cTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTcgg                                                                                                                                     >  1:1077824/1‑147 (MQ=9)
                   aaCTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCACCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCAGCCCGAAGGTCGGACa                                                                                                                                  >  1:287771/1‑149 (MQ=255)
                     cTGCCGCGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAg                                                                                                                                <  1:1244035/149‑1 (MQ=255)
                      tGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCGGACAAgg                                                                                                                               >  1:913515/1‑149 (MQ=38)
                        ccGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACTGATGTTAATTTCGGCACCCCGTAGGTCAGACAAGGCg                                                                                                                             <  1:346856/149‑1 (MQ=14)
                        ccGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCg                                                                                                                             <  2:841993/149‑1 (MQ=255)
                            gAAGTGCAGCGCCCCGTAGGTCAGACAGGGGGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGGCa                                                                                                                         <  1:1343119/149‑1 (MQ=14)
                                tGCGGCAGCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCAcgcc                                                                                                                     <  2:42080/149‑1 (MQ=255)
                                  cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCAcgccgc                                                                                                                   <  1:1282257/149‑1 (MQ=255)
                                  cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTATGAAGTGCGGCACCCCGTAGGGCGGACAAGGCGGTTAcgccac                                                                                                                   >  2:1229547/1‑147 (MQ=255)
                                    gCACCCCGTAGGTCAGACAAGGGGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCag                                                                                                                 >  1:727539/1‑148 (MQ=255)
                                      aCCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCTGTCACGCCGCATcc                                                                                                               <  1:850774/149‑1 (MQ=255)
                                        cccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCGGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTTACGCCGCATCCgc                                                                                                             >  2:1223805/1‑148 (MQ=255)
                                         ccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCGCCCCGTAGGTCAGACAAGGCGGATAGGCCGCaactgag                                                                                                            >  2:147476/1‑143 (MQ=11)
                                                 cAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACg                                                                                                    <  1:614971/149‑1 (MQ=255)
                                                  aGACAAGGCGGTCACGCCGCATCCGACACACAACGCCAGAGCCGGTTGCCTGATGCGCCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGNTCACGCCGCCTCCGACATCAACGcc                                                                                                  >  1:929720/1‑149 (MQ=14)
                                                     cAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCAACGCCTGa                                                                                               <  2:929720/149‑1 (MQ=255)
                                                      aaGGCGGTCACGCCACATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCCTCCGACATCCAACGcccaa                                                                                               >  1:185111/1‑147 (MQ=255)
                                                               aCGCCGTTTCCGACATCCAACGCCCGCGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCCGGCCTCGACCGCTGTGAAGTGCTGCACCCCGTAGGTCGGACAAGGCGGTCACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGc                                                                                      <  2:727539/149‑1 (MQ=11)
                                                               aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGTCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCAGCACCCCGTAGGTCAGACAAGGCGGTTACGCCGCATCCGCCATCCAACGACCGAGGCGGTTGc                                                                                      >  1:243602/1‑149 (MQ=14)
                                                                  ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTg                                                                                   >  2:900521/1‑149 (MQ=255)
                                                                  ccgcATCCGACACCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTg                                                                                   <  1:1152866/149‑1 (MQ=255)
                                                                   cgcATACGTCATCCAACCCCCGAGCCGGTTGCCTGAGGCGACGTTGTCGCGCCTTATCAGGCCTACACCGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTgc                                                                                  <  2:613138/149‑2 (MQ=17)
                                                                       tCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCa                                                                                                                         <  1:764664/106‑1 (MQ=255)
                                                                       tCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCa                                                                                                                         >  2:764664/1‑106 (MQ=255)
                                                                        ccGACATCCACCGCCCGAGCTGGTGGCCTGATGTGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGCAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCCGACCCGACATCCAACTCCCGAGCCGCTTGCCTGATGCGa                                                                             <  1:1231580/149‑1 (MQ=17)
                                                                         cGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCGGCCAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGAc                                                                            >  2:199378/1‑149 (MQ=255)
                                                                         cGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGAc                                                                            >  1:1367397/1‑149 (MQ=255)
                                                                                    ccacGAGCCGGTTGCCTGCTGCGACGCTGGCGCGTCTTATCAGCCCTACACGGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                 <  2:1059501/146‑1 (MQ=255)
                                                                                        gAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCa                                                                                                                                                  >  1:810580/1‑64 (MQ=37)
                                                                                           ccGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAg                                                          <  1:304978/149‑1 (MQ=255)
                                                                                                   cTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTacac                                                  >  1:92152/1‑149 (MQ=255)
                                                                                                    gggtGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACTCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAcc                                                 <  1:197423/146‑1 (MQ=255)
                                                                                                      aTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGATCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGc                                               >  2:71770/1‑149 (MQ=255)
                                                                                                       tGCGACGCTGGCGCGTCTTATCAGGCCTACATCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCt                                              <  1:390587/149‑1 (MQ=255)
                                                                                                             gCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCCCCACCGCTCTGAAg                                        >  1:679060/1‑149 (MQ=255)
                                                                                                                 gcgcGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCGGATAAGGCGGTCACGCCGGCTCCGACATCCACCGCCCGCGCCGGTTGCCTGATGCTATGCTGGCGCGTGCTATCAGGCCTACACCGCGGGGAAGTGCt                                    >  1:919935/1‑149 (MQ=11)
                                                                                                                   gcgTCTTATTAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGCCATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGGCTTGTCAGGCCTACACCGCTGCGAAGtcctcc                                  >  1:288875/1‑149 (MQ=14)
                                                                                                                   gcgTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTGCACCGCTGTGGAGTGCTcc                                  >  1:353963/1‑149 (MQ=255)
                                                                                                                   gcgTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGCGGAGTGcccc                                  >  1:9703/1‑149 (MQ=255)
                                                                                                                        ttATCAGGCCTACACCGCTGTGAAGTGAGGCACCCCGTAGGTGAGCCAAGGCGGTCACGCCGAATCCGACATCCAACGCCCGAGCCGGTTGACTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGCAGTGCTGCAcgcc                             >  1:1009977/1‑149 (MQ=14)
                                                                                                                           tgaGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTa                          <  2:914020/147‑1 (MQ=255)
                                                                                                                           tCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCCCGAAGCTACTGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTa                          <  1:1033934/149‑1 (MQ=14)
                                                                                                                            catgCCTACGCCGATTTGTAGTGCGACGCCCCGTAGGGCGGATAAGGCGGTAACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                         <  2:789935/146‑1 (MQ=11)
                                                                                                                             aGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCCGCCCTCCACCGCTGCGAACTGCTCCACCCCGTCgg                        >  1:139308/1‑149 (MQ=14)
                                                                                                                             aGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                        >  1:139154/1‑149 (MQ=255)
                                                                                                                             aGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                        >  2:1067209/1‑149 (MQ=255)
                                                                                                                               gCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTc                      <  1:834175/149‑1 (MQ=255)
                                                                                                                                gcTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCg                     <  2:164724/148‑1 (MQ=255)
                                                                                                                                  tACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCGGGCCTACACCGCTGTGAACTGCTCCACCCCGTAGGTCGGa                   >  1:1277772/1‑149 (MQ=255)
                                                                                                                                     acCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATaa                >  2:29453/1‑149 (MQ=255)
                                                                                                                                         ccgtgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGAGATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg            <  2:869921/147‑1 (MQ=255)
                                                                                                                                         cTGTTAAGTGCGTCACCCCGTACTGTATTCAAGGCGGTCACGCCCCACCCGACATCCAACGCCCGATCCGGTTGCCTGACGCGACGCTGGCTCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg            <  2:287771/149‑1 (MQ=14)
                                                                                                                                         cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg            >  1:558956/1‑149 (MQ=255)
                                                                                                                                          tgtgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCgg           >  2:663445/1‑149 (MQ=40)
                                                                                                                                           gtgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATATGACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGt          <  1:331459/149‑1 (MQ=255)
                                                                                                                                            tgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGCCGCTGTGAAGTGCTCCACCCCGTAGGGCGGATAAGGCGGtt         >  1:4667/1‑149 (MQ=255)
                                                                                                                                             gAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTa        <  1:663445/149‑1 (MQ=255)
                                                                                                                                                 tGCGGCACCCCGTAGGTCAGACAGGGCGGTCACGCNGCATCCGACATCNCCTAACCTACTCCGTTGCCTGATGCGACGCTGGCGCGTCNTATCNGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgcc    <  1:272317/149‑1 (MQ=14)
                                                                                                                                                   cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgccgc  <  2:1077824/149‑1 (MQ=255)
                                                                                                                                                   cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgccgc  >  1:945151/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGC  >  NC_000913_3_pae_tpiA/2304374‑2304669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: