Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 3,440,435 C→A 36.5% D199Y (GAC→TAC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA3,440,4350CA36.5% 134.2 / 112.2 131D199Y (GAC→TAC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (36/47);  new base A (21/27);  total (57/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 135 total aligned reads displayed.

TCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCGGCACATAA  >  NC_000913_3_pae_tpiA/3440287‑3440577
                                                                                                                                                    |                                                                                                                                              
tctGGTTTCTCTTCTTTCCCTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTc                                                                                                                                                <  1:230535/149‑1 (MQ=255)
tctGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTc                                                                                                                                                >  1:349580/1‑149 (MQ=255)
tctGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTc                                                                                                                                                >  1:283799/1‑149 (MQ=255)
tctGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGCTGGTTTCCATTTCGATGACCAGCTTGTc                                                                                                                                                <  2:810928/149‑1 (MQ=255)
  tGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCa                                                                                                                                              <  1:410364/149‑1 (MQ=255)
      ttCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTc                                                                                                                                          <  1:45437/149‑1 (MQ=255)
      ttCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTc                                                                                                                                          <  2:658265/149‑1 (MQ=255)
      ttCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCCATGACCAGCTTGTCCAGGTc                                                                                                                                          >  2:431339/1‑149 (MQ=255)
        cccttcttTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCgg                                                                                                                                        <  1:476700/147‑1 (MQ=255)
           ttcttTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCCTTTCGATGACCAGCTTGTACAGGTCGGTAc                                                                                                                                     >  2:187566/1‑149 (MQ=255)
           ttcttTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCCGCTTGTCCAGGTAGGTAc                                                                                                                                     >  2:753837/1‑149 (MQ=255)
                tCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTgc                                                                                                                                >  1:1323334/1‑148 (MQ=255)
                    ttCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATGGGTTGTGCCGTTGGTTTCCATTTCGATGACCACCTTGTCCAGTTAGGTACGCTGTTCTa                                                                                                                            >  2:261785/1‑149 (MQ=255)
                    ttCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTa                                                                                                                            <  1:173117/149‑1 (MQ=255)
                    ttCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCACCTTGTCCAGGCCGGTCCGCTGTTCtc                                                                                                                            >  2:261730/1‑148 (MQ=255)
                    tgcAGTCTGACGGACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTa                                                                                                                            <  2:955079/147‑1 (MQ=255)
                     tCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTac                                                                                                                           <  1:1151954/149‑1 (MQ=255)
                         gCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGCATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACAcgc                                                                                                                       >  1:910773/1‑149 (MQ=255)
                          cTGACGTAAATCACGTAACTCAACGAAAGCTTCCAGTTATTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGTCCAGCTTGTCCATGTCGGTACGCTGTTCTACAcgcg                                                                                                                      <  2:570846/149‑1 (MQ=255)
                            gACGTACATCCCGTAAGTCAACGCAACTTTCCTGTGGTGCGGCCGGGCCCTGGGCCGGACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCgct                                                                                                                    <  1:1390859/149‑1 (MQ=255)
                                  cATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCa                                                                                                              >  1:115109/1‑149 (MQ=255)
                                   aTCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCaa                                                                                                             >  2:970131/1‑149 (MQ=255)
                                    tCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCATCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAAGTCGGGACGCAGTTCTACACGCGCTGCTTCAAc                                                                                                            >  2:1208595/1‑149 (MQ=255)
                                     cACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACa                                                                                                           >  2:435271/1‑149 (MQ=255)
                                     cACGTAAGTCAACGAAAGCTTCCAGTTGATCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTCTCCATTGCGACGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACa                                                                                                           <  1:1040866/149‑1 (MQ=255)
                                       tctAAGTTAACTAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACAtt                                                                                                         <  2:1420753/147‑1 (MQ=255)
                                       cGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACAtt                                                                                                         <  1:1295504/149‑1 (MQ=255)
                                          aaGTCAACGAAAGCTTCCAGTTGTTGAGCCAGAATGGTTGCCGCACGACCAAGCGCCTCTTCAGGATCGATTGTGCCGATGCTTTGCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTa                                                                                                      <  1:1260696/149‑1 (MQ=255)
                                          aaGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTa                                                                                                      <  2:74975/149‑1 (MQ=255)
                                          aaGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTa                                                                                                      <  2:747664/149‑1 (MQ=255)
                                          aaGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTa                                                                                                      <  1:798045/149‑1 (MQ=255)
                                          aaGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATGTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTa                                                                                                      <  1:1187818/149‑1 (MQ=255)
                                          aaGTCAACAAAAGCTTCCCGTTGTTAATTCAGAATTGTTGCTGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTa                                                                                                      <  2:434849/149‑1 (MQ=255)
                                           attCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAg                                                                                                     <  2:943339/147‑1 (MQ=255)
                                           aGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGAATACTAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAg                                                                                                     <  1:1111028/149‑1 (MQ=255)
                                             tCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGATTCAACATTGTAGGc                                                                                                   <  2:494291/149‑1 (MQ=255)
                                             tCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGACTGTTACGCGGTTCTACACGCGCTGCTTCAACATTGTAGGc                                                                                                   >  1:265527/1‑149 (MQ=255)
                                             tCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGATTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGCTCTACACGCGCTGCTTCCACATTGTAGGc                                                                                                   >  2:983416/1‑149 (MQ=255)
                                             tCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTACCGTTCGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGc                                                                                                   <  1:1097469/149‑1 (MQ=255)
                                               aatgAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCaa                                                                                                 <  2:503891/146‑1 (MQ=255)
                                               aaCGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGGCGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCGGTTTTCCACGCGGTGCTCGAACATTGTAGGCaa                                                                                                 >  1:990607/1‑149 (MQ=255)
                                               aaCGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCaa                                                                                                 <  1:412083/149‑1 (MQ=255)
                                               aaCGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCaa                                                                                                 <  1:435271/149‑1 (MQ=255)
                                               aaCGAAAGCTTCAAGTTGATCAGGCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCaa                                                                                                 >  2:169495/1‑149 (MQ=255)
                                                 cGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATa                                                                                               <  1:1278677/149‑1 (MQ=255)
                                                   gaaGCTTCGACTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGCATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACg                                                                                             <  2:718793/148‑1 (MQ=255)
                                                      gCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTc                                                                                          <  1:179003/149‑1 (MQ=255)
                                                        ttCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTTCTTCAACATTGTAGGCAATACGCTCca                                                                                        >  1:306126/1‑149 (MQ=255)
                                                          ccAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCcaca                                                                                      <  2:114773/149‑1 (MQ=255)
                                                           cAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAg                                                                                     <  2:1014000/149‑1 (MQ=255)
                                                             gttgttCAGCCAGAATGGTTGCCGGACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAggg                                                                                   <  1:475814/149‑1 (MQ=255)
                                                             gttgttCAGCCAGAATGGTGGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAggg                                                                                   <  1:476400/149‑1 (MQ=255)
                                                               tgttCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCt                                                                                 <  1:235187/149‑1 (MQ=255)
                                                                   cAGCCCGAATGGTTGCCGCACGACGAACCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAg                                                                             <  2:981828/149‑1 (MQ=255)
                                                                    aGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGc                                                                            >  1:522686/1‑149 (MQ=255)
                                                                     gCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGtttcg                                                                           <  2:1157095/149‑5 (MQ=255)
                                                                         gAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCAGTTGGTATCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCg                                                                       >  1:1343251/1‑149 (MQ=255)
                                                                          aaTGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTATAGGCAATACGCTCCACAGTGCTGTAGCATGCGt                                                                      >  2:1331285/1‑149 (MQ=255)
                                                                            tGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCg                                                                    <  1:1415683/149‑1 (MQ=255)
                                                                             ggTTGCCGGACGACGTATCGCCTCTTCAGGATGGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTAAGCCCGCTGCGTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGa                                                                   <  2:729342/149‑1 (MQ=255)
                                                                             ggTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGa                                                                   <  1:928036/149‑1 (MQ=255)
                                                                                 gCCGTAGGACGAATTGCCTCTTCGGGGTCGATTGTGCCGTTGGTTTCTATTTCGATGACCAGCTTGTCTTGGTCGGTTCGCTGTTCTACCCGTGTTGTTTCAATATTGTAGGCAATACGCTCCACAGGGCTGTTGCATGCGTCGACtag                                                               <  2:819291/149‑1 (MQ=255)
                                                                                 gCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGGTTACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACcag                                                               >  1:1111120/1‑149 (MQ=255)
                                                                                 gCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACcag                                                               >  1:460168/1‑149 (MQ=255)
                                                                                  ccGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTCGCATGCGTCGACcagc                                                              >  1:1321991/1‑149 (MQ=255)
                                                                                      acgacgAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGAc                                                          >  1:1142899/1‑149 (MQ=255)
                                                                                            actCGCCTCTTCAGGTTCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGa                                                    <  1:549595/147‑1 (MQ=255)
                                                                                             aTCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCGGACGGCCGAt                                                   >  1:955936/1‑149 (MQ=255)
                                                                                              tttccTCTTCGGGATCGATTGTGCCGTTGGGTTCCATTTAGATGTCCGGTTTGTATGGGTCTGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGAtt                                                  <  2:663754/146‑1 (MQ=255)
                                                                                                 ccTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTggg                                               >  1:104970/1‑149 (MQ=255)
                                                                                                 ccTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGACGACCAGCAGACGGCCGATTggg                                               <  1:810792/149‑1 (MQ=255)
                                                                                                  ctctTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGgc                                              >  1:569489/1‑149 (MQ=255)
                                                                                                    ctTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGgcgc                                            <  2:1256151/149‑1 (MQ=255)
                                                                                                         ggATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGGCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATTCGCTCCACAGGACTGTAGCATGGGTCGACCAGTCGACGGACGATTGGgcacccacg                                       >  2:65160/1‑142 (MQ=255)
                                                                                                          gATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACCCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGGAGACCAGCAAGCGGCCGATTAGGCGCTccgat                                      >  2:1214231/1‑145 (MQ=255)
                                                                                                          gATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCAtct                                      >  1:1280888/1‑149 (MQ=255)
                                                                                                            tCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCAtcttc                                    <  2:910773/149‑1 (MQ=255)
                                                                                                                ttGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCACTCATCTTCTTcc                                <  1:956552/149‑1 (MQ=255)
                                                                                                                  gtgCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCga                              <  2:920597/149‑1 (MQ=255)
                                                                                                                   tggcGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCtaa                             <  2:1306913/146‑1 (MQ=255)
                                                                                                                      cGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAACATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCgaatga                          >  2:1063167/1‑149 (MQ=255)
                                                                                                                       gTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCgaatgaa                         >  2:1258639/1‑149 (MQ=255)
                                                                                                                       gTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCCCTCATCTTCTTCCgaatgaa                         >  2:1258869/1‑149 (MQ=255)
                                                                                                                         tgttttCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAAtt                       <  2:471687/146‑1 (MQ=255)
                                                                                                                           gTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATTCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCg                     <  1:639629/149‑1 (MQ=255)
                                                                                                                           gTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCg                     <  1:591306/149‑1 (MQ=255)
                                                                                                                           gTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCGGTAGCATGCGTCGACCAGCAGACGGCCGCTTGGGCCCGCATCTTCTTCCGAATGAATTCg                     >  2:793381/1‑149 (MQ=255)
                                                                                                                           gTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCg                     >  1:1219332/1‑149 (MQ=255)
                                                                                                                            tttCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCgg                    >  2:915091/1‑149 (MQ=255)
                                                                                                                            tttCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGCCAATAGGCTCCACAGGGCTGTAGCATGCGTCGACAAGTAGACGGCCGGTTGGGCGCGCATCATCTTCCAAAGCAATTggg                    >  1:298286/1‑149 (MQ=255)
                                                                                                                            tttCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATCCGCTCCACAGGGCTGTAGCGTACGTCGACCAGCGCACGGACGATTGACGGTTCATCTTATTCCGAATGACTGCgg                    >  2:592388/1‑149 (MQ=255)
                                                                                                                                  tttCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGaa              >  1:1077131/1‑149 (MQ=255)
                                                                                                                                      gATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCg          <  2:104970/149‑1 (MQ=255)
                                                                                                                                        tGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAACAGACGGCCGATTAGGCGCTCATCTTCTTCCCAATGAATTCTGGGAGAAGCAGGc        >  2:274592/1‑149 (MQ=255)
                                                                                                                                         gACCAGCTTGTCCAGGTCGGTAGGCTGTTTTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGCCCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAAGTCGGCTTGAAGCCGGcc       >  1:723685/1‑148 (MQ=255)
                                                                                                                                         gACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCGGca       <  2:1323334/149‑1 (MQ=255)
                                                                                                                                          aCCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCACCATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCGGcac      >  1:914628/1‑149 (MQ=255)
                                                                                                                                          aCCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCGGcac      >  1:914458/1‑149 (MQ=255)
                                                                                                                                            cAGCGTGTCCAGGTCGGTACGCTGTTCTACACGCGGTGCTTCAACATTGTAGTGAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCGGCACAt    <  2:841664/149‑1 (MQ=255)
                                                                                                                                              gCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCGGCACATaa  >  1:170293/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
TCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCGGCACATAA  >  NC_000913_3_pae_tpiA/3440287‑3440577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑