Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 3,979,412 C→T 5.4% P271L (CCG→CTG)  wzyE → putative ECA polysaccharide chain elongation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA3,979,4120CT 5.4% 251.7 / 2.2 86P271L (CCG→CTG) wzyEputative ECA polysaccharide chain elongation protein
Reads supporting (aligned to +/- strand):  ref base C (48/33);  new base T (2/3);  total (50/36)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

GCTGGATTTCGTTGTGGATGCTGGCGGCGGCGGGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTT  >  NC_000913_3_pae_tpiA/3979265‑3979560
                                                                                                                                                   |                                                                                                                                                    
gCTGGATTTCGTTGTGGATGCTGGCGGCGGCGGGCGTGCTGGGGATTGTTGGAATAAACGGTCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCg                                                                                                                                                     >  1:1109273/1‑149 (MQ=255)
 cTGGATTTCGTTGTGGATGCTGGCGGCGGCGGGCGAGCTGGGGATTGGTGGCATGTTCTGGCTGGCAATAACACGCTATGGAAGTAATGTGAGCGACGATGCAGGGTTCTATACGGTCCTCTCTCCCCCTCGCGCCACCTTCTCGccct                                                                                                                                                    >  2:1117059/1‑147 (MQ=255)
   ggATTTCGTTGTGGATGCTGGCGGCGGCGGGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGGGACACCTTCTTGCCGTgg                                                                                                                                                  >  1:542793/1‑149 (MQ=255)
   ggATTTCGTTGTGGATGCTGGCGGCGGCGGGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTgg                                                                                                                                                  <  2:242484/149‑1 (MQ=255)
   ggATTTCGTTGTGGATGCTGGCGGCGGCGGGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTgg                                                                                                                                                  <  1:664584/149‑1 (MQ=255)
     aTTTCGTTGTGGATGCTGGCGGCGGCGGGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGga                                                                                                                                                >  1:87446/1‑149 (MQ=255)
      tttCGTTGTGGATGCTGGCGGCGGCGGGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGgag                                                                                                                                               <  2:543533/149‑1 (MQ=255)
           ttGTGGATGCTGGCGGCGGCGGGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCTCGACACCTTCTCGCCGTGGGAGAATCt                                                                                                                                          >  2:1033394/1‑149 (MQ=255)
                gATGCTGGCGGCGGCGGGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGt                                                                                                                                     >  1:1313833/1‑149 (MQ=255)
                 agtgtGGCGGCGGCGGGCGGGCTGGGGATTGTTGGCATTGTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGtt                                                                                                                                    <  2:1258557/145‑1 (MQ=255)
                       gcggcggcgGGCGTGCTGGGGATTGTTGGCATGGTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGtt                                                                                                                              <  1:1302186/149‑1 (MQ=255)
                        cggcggcggGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGGGAGCGGCGATGAAGCGTTCTATACGTTTTTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTg                                                                                                                             <  2:779167/149‑1 (MQ=255)
                         ggcggcggGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGc                                                                                                                            <  1:1259125/149‑1 (MQ=255)
                          gcggcggGCGTTCTGGTGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCa                                                                                                                           <  1:1314866/149‑1 (MQ=255)
                          gcggcggGCGTGCTGGGGATTTCTGGCATGTTCTGGCTGGCACTAAACCGCTATGGAATGAATGTGAGCGGTGATGAAGCGTTCTATACGTTGCTCTATCTCACTCGCGACACCTGCTCGCCGTGGGAGAATCTGGCGTTTCTGTTGCa                                                                                                                           <  1:559974/149‑1 (MQ=255)
                           cggcggGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAg                                                                                                                          <  2:1123623/149‑1 (MQ=255)
                             gcgggcgTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGaa                                                                                                                        <  1:906215/149‑1 (MQ=255)
                               gggcgTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGAAGAACt                                                                                                                      >  2:203775/1‑149 (MQ=255)
                                ggcgTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTa                                                                                                                     >  2:941006/1‑149 (MQ=255)
                                      cTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCTGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGacaa                                                                                                               <  2:758647/149‑1 (MQ=255)
                                      cTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCTGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGacaa                                                                                                               <  2:758739/149‑1 (MQ=255)
                                      cTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCTGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGacaa                                                                                                               >  1:758647/1‑149 (MQ=255)
                                      cTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCTGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGacaa                                                                                                               >  1:758739/1‑149 (MQ=255)
                                       tGGGGATTGTTGGCATGGTGTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATGCGTTTCTGTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGacaac                                                                                                              <  2:518113/149‑1 (MQ=255)
                                          ggATTGTGGGCAGGTTATGGCTGGCAATAAACCGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACCCCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATc                                                                                                           <  1:290869/149‑1 (MQ=255)
                                                  ggCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCa                                                                                                   <  2:321605/149‑1 (MQ=255)
                                                     aTGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAggg                                                                                                >  1:101606/1‑149 (MQ=255)
                                                      tGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGGATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGc                                                                                               >  2:827174/1‑149 (MQ=255)
                                                      tGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGc                                                                                               >  2:379378/1‑149 (MQ=255)
                                                      tGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGCGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGc                                                                                               >  1:1027790/1‑149 (MQ=255)
                                                        ttCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCt                                                                                             >  1:1399933/1‑149 (MQ=255)
                                                        ttCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGCTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCt                                                                                             >  1:425047/1‑149 (MQ=255)
                                                          ctggctggCCGTAAAACTCTATGTAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCGGTTTCAAACCTCCCATAACATCGACTTCCAGGGCCTgg                                                                                           <  2:375510/149‑1 (MQ=255)
                                                           tgcctggcACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGc                                                                                          <  1:173740/146‑1 (MQ=255)
                                                             gctggcACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCTCGGCGTTGGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTc                                                                                        >  1:471633/1‑149 (MQ=255)
                                                                ggcACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCaa                                                                                     >  2:589955/1‑149 (MQ=255)
                                                                   aCTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAGTTg                                                                                  >  2:10150/1‑149 (MQ=255)
                                                                   aCTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTg                                                                                  >  1:1269367/1‑149 (MQ=255)
                                                                   aCTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTg                                                                                  >  1:452025/1‑149 (MQ=255)
                                                                    cTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGt                                                                                 >  1:8186/1‑149 (MQ=255)
                                                                        aaCGCTATGGAATGAATGTGAGCGGCGATGAAGCGGTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAGTCTGGCGTTGCTGTTCCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCtgc                                                                             >  1:1292049/1‑149 (MQ=255)
                                                                         aCGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCcgcg                                                                            >  1:607561/1‑149 (MQ=255)
                                                                                ggAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTa                                                                     <  2:1266319/149‑1 (MQ=255)
                                                                                     tgatgTGAGCGGTGATGAAGTGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCt                                                                <  2:1353354/147‑1 (MQ=255)
                                                                                      aatgTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTCATCTGTCtt                                                               >  2:928970/1‑149 (MQ=255)
                                                                                       atgTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCttt                                                              >  1:602854/1‑149 (MQ=255)
                                                                                        tgtgAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTa                                                             >  1:1008446/1‑149 (MQ=255)
                                                                                          tgAGCGGCGATGAAGCGTTCTATTCGTTTCTCTATCTCACTCGCGACACCTTCTCGCTGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACTACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATc                                                           <  1:1254464/149‑1 (MQ=255)
                                                                                            aGCTGCTATGAAGCGTTCTATACGTTTCTCTAGCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCATGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATccc                                                         <  2:423347/149‑1 (MQ=255)
                                                                                              cggcgATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGCTTGCTATGTCTTTATCCCtt                                                       >  1:518622/1‑149 (MQ=255)
                                                                                              cggcgATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGCTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTTTCCGCGATTTCTATGTCTTTATCCCtt                                                       >  2:114580/1‑149 (MQ=255)
                                                                                                gcgATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTcc                                                     >  1:838868/1‑149 (MQ=255)
                                                                                                gcgATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTcc                                                     >  1:838904/1‑149 (MQ=255)
                                                                                                 cgATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCt                                                    <  2:907670/149‑1 (MQ=255)
                                                                                                 cgATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCt                                                    <  1:233222/149‑1 (MQ=255)
                                                                                                  gATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGACTCACCAGTATGTTCTGCGATTTCTAGGTCTTTATCCCTTCCTg                                                   >  1:963506/1‑149 (MQ=255)
                                                                                                     gAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCt                                                >  2:744553/1‑149 (MQ=255)
                                                                                                     gAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCt                                                >  1:197565/1‑149 (MQ=255)
                                                                                                      aatcGTTCTATACGTTTCTCTACCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTCGCTGTTGCAGAACTAAGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCtg                                               <  1:433973/146‑1 (MQ=255)
                                                                                                      aaGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGATGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGAtg                                               >  2:1139829/1‑149 (MQ=255)
                                                                                                             cTATTCGTTTTTCTATCTGCCTCGCGACACCTTCTCGCCGTTGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCgg                                        <  2:272084/149‑1 (MQ=255)
                                                                                                              tataCGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCggg                                       >  1:1076037/1‑149 (MQ=255)
                                                                                                                taCTTTTCTTTATCTCACTCTCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACTACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTc                                     <  2:397410/149‑1 (MQ=255)
                                                                                                                taCTTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGCATCTGGCTTTGCTGTTGCAGAACTACGACAACTTCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTc                                     <  2:397343/149‑1 (MQ=255)
                                                                                                                   gTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGACCTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGcc                                  >  1:1117731/1‑149 (MQ=255)
                                                                                                                   gTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGCTTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTGGcc                                  >  2:1092672/1‑149 (MQ=255)
                                                                                                                   gTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGcc                                  >  2:955038/1‑149 (MQ=255)
                                                                                                                     ttCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCg                                >  2:800603/1‑149 (MQ=255)
                                                                                                                        tctATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGGGt                             >  1:536125/1‑149 (MQ=255)
                                                                                                                        tctATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGt                             >  2:1389789/1‑149 (MQ=255)
                                                                                                                         ctATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTa                            <  2:197565/149‑1 (MQ=255)
                                                                                                                          tgtctcACTCGCGACACCTTCTCGCCGTGGGAGCATCCGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTCGCGCCAATTGTCCGCACTCTCTATGTCTTTATCCCTTCGAGGCTGTGGACGGGTCGCCGGAGtgt                           >  2:946016/3‑147 (MQ=255)
                                                                                                                          tATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCACTTGTCCGCGCGTTCTATGTCTTGATCCCTTCCTGGCTCTGGCCGGGTCGCCCGAGTag                           >  1:841420/1‑148 (MQ=255)
                                                                                                                           aTCTCACTCGCGACACCTTCTCGCCGGGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCCGGTCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATg                          <  1:197496/149‑1 (MQ=255)
                                                                                                                           aTCTCACTAGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATg                          <  1:125903/149‑1 (MQ=255)
                                                                                                                            tctcGCTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATgg                         <  2:835663/149‑1 (MQ=255)
                                                                                                                            tctcACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATgg                         <  1:164634/149‑1 (MQ=255)
                                                                                                                              tcgcTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTg                       <  2:825017/146‑1 (MQ=255)
                                                                                                                              tcACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCGGTGGCCGGGACGCCCGAGTCTAGTg                       >  2:981753/1‑149 (MQ=255)
                                                                                                                              tcACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTATGTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTg                       >  2:981747/1‑149 (MQ=255)
                                                                                                                                 cccgcgACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGTCTTCCAGGGCCTGGCTCCAATTCTCCGCGGTCTCGGGGGCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTg                    <  2:599039/147‑1 (MQ=255)
                                                                                                                                  tCGCGACACCTTCTCGACGGGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTCCTATGTCTTTATCCCTTCCTGGCTGTGGACGGGGCGCCCGAGCATGGTGCTGa                   >  2:171304/1‑149 (MQ=255)
                                                                                                                                    gcgACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAAc                 >  2:799751/1‑149 (MQ=255)
                                                                                                                                    gcgACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTAGGGTGCTGAAc                 >  1:274152/1‑149 (MQ=255)
                                                                                                                                           cTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCGGCCa          >  1:822515/1‑149 (MQ=255)
                                                                                                                                            ttCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGCCTGTGGCCGGGTCGCCCGAGTATGATGCTGAACtaggcata         >  2:434163/1‑142 (MQ=255)
                                                                                                                                               tcGCCGTTGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAacta      <  1:142295/149‑1 (MQ=255)
                                                                                                                                                   cGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAATATTGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACttt  <  2:686824/149‑1 (MQ=255)
                                                                                                                                                   cGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTTATGAGCTCAGCCGAGTACttt  >  2:49612/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCTGGATTTCGTTGTGGATGCTGGCGGCGGCGGGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTT  >  NC_000913_3_pae_tpiA/3979265‑3979560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: