Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 349,615 T→C 13.5% intergenic (+43/‑397) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA349,6150TC13.5% 227.5 / 20.8 100intergenic (+43/‑397)prpB/prpC2‑methylisocitrate lyase/2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base T (45/39);  new base C (9/5);  total (55/45)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

TCGACACCATGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAA  >  NC_000913_3_pae_tpiA/349472‑349761
                                                                                                                                               |                                                                                                                                                  
tCGACACCATGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTg                                                                                                                                               >  1:432936/1‑149 (MQ=255)
tCGACACCATGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCCACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCCGATGCCTCGCTg                                                                                                                                               >  2:914483/1‑149 (MQ=255)
tCGACACCATGCAGACCCGCAACGAGCTGTACGAAAGCAACAACTACTACCAGTCCGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTAATAGTATTCGACCGCCGCTGCCTGATCCGCCGCTg                                                                                                                                               >  1:495559/1‑149 (MQ=255)
 cGACACCATGCAGACCCGCCACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCAGTAGATGAAAATGGACAGCCGATGCCTGATGCGCCGCTGa                                                                                                                                              >  2:1360491/1‑149 (MQ=255)
 cGACACCATGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGa                                                                                                                                              <  1:951947/149‑1 (MQ=255)
   acacCATGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGAcg                                                                                                                                            >  2:1336726/1‑149 (MQ=255)
   acacCATGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTAGCTGAcg                                                                                                                                            >  1:856464/1‑149 (MQ=255)
    cacCATGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGAcgc                                                                                                                                           >  2:494025/1‑149 (MQ=255)
    cacCATGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGAcgc                                                                                                                                           >  2:494031/1‑149 (MQ=255)
      ccATGCATCCCCGCAACGAGCTGTACGAAAGCAGCAACTCCGACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCATGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGAcccta                                                                                                                                         <  2:889266/149‑5 (MQ=255)
      ccATGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGa                                                                                                                                         <  2:457911/149‑1 (MQ=255)
       caagCAAACCTGCAACGAGCTGTACGAAATCATCAACTACCACCAGTACGAAGAGAAGCTCTACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGAc                                                                                                                                        <  2:452964/146‑1 (MQ=255)
             gACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCa                                                                                                                                  >  2:882644/1‑149 (MQ=255)
             gACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCa                                                                                                                                  <  1:921375/149‑1 (MQ=255)
              aacggCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAg                                                                                                                                 <  2:1058704/145‑1 (MQ=255)
                       tacctctACGAAAGCATCAACTACTACCGTGACGAAGAGAAGGCCGACATCTTGTTTGACCGTAGCCAGGTGAAATAAAAAGCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGGCGCTTGCGCGACTTATCAGGCCTACGAg                                                                                                                        <  2:1012882/143‑1 (MQ=255)
                         gCTGTACGAAAGCATCACCTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGt                                                                                                                      <  2:1044971/149‑1 (MQ=255)
                          cTGTACGAAAGCATCAATATCTTCCAGTACGAAGCTAAGCTCGACAGGTTGTCTGCCGGTGGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTAAGAGGTg                                                                                                                     <  2:663211/149‑1 (MQ=37)
                          cTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGCCGCGACTTATCCGGCCTACGAGGTg                                                                                                                     >  1:1418425/1‑149 (MQ=255)
                           tGTACGACAGCATCAACTACTACCAGAACTAAGAGAAGCTCGACACCCTGTTTGCCCGTAGCCAGGTGAAATAACCAACACCCGCTGATTGTATTCGACACCCGATGCCTGATACATCGCTGACGCGACTTATCAGGCCCACGAGCTGc                                                                                                                    >  2:98290/1‑149 (MQ=255)
                           tGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGc                                                                                                                    >  2:530736/1‑149 (MQ=255)
                           tGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGAGGTGc                                                                                                                    >  1:20905/1‑149 (MQ=255)
                           tGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTAGACAGCAGATGCCTGATGCGCCGCTGCCGTGCCTTATCAGGCCTACGGGGTGc                                                                                                                    >  2:149729/1‑149 (MQ=38)
                              aCGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCATTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACt                                                                                                                 <  1:982264/149‑1 (MQ=255)
                                gAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGa                                                                                                               >  1:1198526/1‑149 (MQ=255)
                                  aaGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAAc                                                                                                             <  2:954188/149‑1 (MQ=255)
                                     cATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGt                                                                                                          >  1:128513/1‑149 (MQ=255)
                                     cATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGt                                                                                                          >  1:1414354/1‑149 (MQ=255)
                                       tCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAg                                                                                                        >  1:589981/1‑149 (MQ=255)
                                       tCAAATACAACCAGGACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAg                                                                                                        <  2:174311/149‑1 (MQ=255)
                                        cAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCACGTTGATTGTATTCGACAACCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAgg                                                                                                       >  2:1219286/1‑149 (MQ=255)
                                          actactACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTc                                                                                                     >  2:499712/1‑149 (MQ=255)
                                            tactacCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCgg                                                                                                   >  1:506016/1‑149 (MQ=255)
                                             actacCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa                                                                                                  <  1:1057764/149‑1 (MQ=255)
                                              ctacCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGAt                                                                                                 >  2:1257171/1‑149 (MQ=255)
                                              ctacCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGAt                                                                                                 >  1:746374/1‑149 (MQ=255)
                                               ttccAGTACGAATAGAAGCTCGACAACCTGTTTGCCCGTAGCCAAGTGAAATAAAAGACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATa                                                                                                <  1:1274419/147‑1 (MQ=25)
                                                acCAGTACGGAGCGAAGCTCGGCCACCGGTGTGCCCGTAGCCCGGTGCAATAACAAATGCTTGCTGATGGTATTCGCCAGCCGATGCCCGATGCGTCGCTGACGCGACTTATCAGGCTTACGAGGTGCACTGAACTGTAGGTCGGATaa                                                                                               <  2:866968/149‑1 (MQ=18)
                                                  cAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGa                                                                                             >  2:1329742/1‑149 (MQ=255)
                                                   aGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGATGACTCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGAc                                                                                            <  2:948848/149‑1 (MQ=255)
                                                     tACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACgg                                                                                          <  2:500424/149‑1 (MQ=255)
                                                      aCGAAGAGAAGCTCGACAACCGGTTTGACCGTAGGCAGGTTAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCATGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGa                                                                                         <  2:385263/149‑1 (MQ=25)
                                                       cGAAGAGAAGCTCGACAACCTGGTTGCCCGTAGCCAGGTGAAATAAACCACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGAt                                                                                        <  1:894442/149‑1 (MQ=255)
                                                           gaagaGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAACACCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTTACGCGACTTATCAGGCCTACGAGGCGCACTGAACGGGTGGTCGGATAAGACGGATGGCg                                                                                    <  2:1011829/145‑1 (MQ=25)
                                                             gaAGCTAGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTAATGCGTCGCTGACGCGACTTATCAGGCCTAAGAGGTGCACAGAACTGTAGGTCGGATAAGACGGATAGCGTc                                                                                  >  2:21415/1‑149 (MQ=25)
                                                                 cTCGACAACCTGTTTGCCCGTAGACAAGTGAAATAAAAACCGCCCGTTGATTGTAGTCGACAGCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGGCTACGTGGTGCACGGAACGGTAGGTCGGATAACCCGGATGGCGGCGCac                                                                              >  1:817976/1‑148 (MQ=21)
                                                                  tCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATc                                                                             >  1:1315697/1‑149 (MQ=255)
                                                                  tCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCACGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATc                                                                             >  2:238899/1‑149 (MQ=255)
                                                                    gACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCg                                                                           >  2:1332712/1‑149 (MQ=255)
                                                                    gACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCg                                                                           >  1:857249/1‑149 (MQ=255)
                                                                     aCAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa                                                                          >  1:127450/1‑149 (MQ=18)
                                                                       aaCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACa                                                                        >  1:1324298/1‑149 (MQ=255)
                                                                       aaCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACa                                                                        >  2:1294924/1‑149 (MQ=255)
                                                                         ccTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTCCGGGGTGCACTGAACTGTAGGACGGATAAGACGCATAGCGTCGCATCCGCCAAc                                                                      >  2:576858/1‑149 (MQ=18)
                                                                         ccTGATTGCCCGGAGCCAGGTGAAATAAAAACCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAAc                                                                      <  1:1234362/149‑1 (MQ=255)
                                                                          cTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAAcc                                                                     >  1:167097/1‑149 (MQ=255)
                                                                             tttGCCCGTAGCCAGGTGAAATAAAAAGCGCCCGTTGATTGTATTCGACAGTCGATGCCTGATGCGTCGCTGACGCGACTTATCATGCCTACGAGGTGCACTCAACGGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGAt                                                                  <  1:123303/149‑1 (MQ=25)
                                                                               tGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGc                                                                <  2:490408/149‑1 (MQ=255)
                                                                                 cccGTAGCCAGGTGAAATAAAACACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGTGTCGCATCCGACAACCGATGCCt                                                              <  1:879905/149‑1 (MQ=255)
                                                                                 cccGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGTGTCGCATCCGACAACCGATGCCt                                                              <  1:880020/149‑1 (MQ=255)
                                                                                  ccGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTg                                                             >  2:666786/1‑149 (MQ=255)
                                                                                   cGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGACGCTAACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCAAACAACCGAttcgtgc                                                            >  2:1072570/1‑143 (MQ=21)
                                                                                     tAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATg                                                          >  2:296509/1‑149 (MQ=14)
                                                                                        ccAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATgcgc                                                       <  1:855672/149‑1 (MQ=255)
                                                                                          aGGTTAAAAAAAAAACGCCCGTTGATTGGATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGcgccg                                                     <  1:1080958/149‑1 (MQ=255)
                                                                                           ggTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGcgccgc                                                    <  1:1208755/149‑1 (MQ=255)
                                                                                           ggTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGcgccgc                                                    >  1:652457/1‑149 (MQ=255)
                                                                                             tGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTg                                                  >  1:1073964/1‑149 (MQ=14)
                                                                                              gAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGa                                                 >  1:581647/1‑149 (MQ=255)
                                                                                               aaaTAAAGAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAc                                                <  2:1316420/149‑1 (MQ=255)
                                                                                                aaTAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACg                                               <  1:1112722/149‑1 (MQ=255)
                                                                                                  tAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTg                                             >  2:337192/1‑149 (MQ=255)
                                                                                                   aaaaaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGa                                            <  2:1363073/149‑1 (MQ=255)
                                                                                                    aaaaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATAGCGTCGCATCCGACAACCAATGCCTGATGCGACGgtgacgtgac                                           >  2:338048/1‑149 (MQ=255)
                                                                                                     aaaaCGCTCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGTGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACt                                          <  1:97302/149‑1 (MQ=255)
                                                                                                          gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGa                                                                                             <  1:106641/93‑1 (MQ=255)
                                                                                                          gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGa                                                                                             >  2:106641/1‑93 (MQ=255)
                                                                                                          gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa                                     <  1:551731/149‑1 (MQ=255)
                                                                                                          gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa                                     >  1:1095053/1‑149 (MQ=255)
                                                                                                          gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa                                     <  2:1234441/149‑1 (MQ=255)
                                                                                                             cGTTGCTTGTATTCTACAGCCGATGCCTGATGAGTAGGTGACGCGCACTATATGCGCTGCGGCGGGCACTGAACTGTAGGTCGGAGAGGACCGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGc                                  <  2:1002077/149‑1 (MQ=14)
                                                                                                              gTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGcc                                 >  1:1295488/1‑149 (MQ=255)
                                                                                                               ttGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGCCTTATCAGGCCt                                >  1:1353464/1‑149 (MQ=255)
                                                                                                                  aTTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCTGCAACCGTTGTCTTGTGCGCCTTTTACTTGACTTAGCATGCCTACg                             >  1:660195/1‑149 (MQ=17)
                                                                                                                   ttGTATTCGAGAGCCGATGCCTGGTGCGTCGCTGACGCGACTTATCAGGCCTAGGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACgg                            <  2:147336/149‑1 (MQ=255)
                                                                                                                   ttGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGTAGTGACTTATCAGACCTACgg                            >  2:427304/1‑149 (MQ=255)
                                                                                                                     gTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGCCAACCGATGCCTGATGCGCCGCTGCCGGGACTTTTCAGGCCCACgggg                          >  2:255941/1‑149 (MQ=17)
                                                                                                                         tCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCa                      <  1:1133464/149‑1 (MQ=255)
                                                                                                                         tCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCa                      >  1:231970/1‑149 (MQ=255)
                                                                                                                           gACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACt                    >  1:650808/1‑149 (MQ=255)
                                                                                                                              aaccGATGCCTGGTGCGTCGCTGACACGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGaa                 <  2:608626/147‑1 (MQ=255)
                                                                                                                                 cGATGCCTGATGCGTCGCTGACGCGNCTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTg              <  1:934166/149‑1 (MQ=255)
                                                                                                                                   aTGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGa                                                                                             >  2:455057/1‑68 (MQ=255)
                                                                                                                                    tGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAg           <  1:573904/149‑1 (MQ=255)
                                                                                                                                       ctggtccGTCGCAGACGCGACTTATCAGGCCTACGAGGGGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTc        <  2:1418425/143‑1 (MQ=255)
                                                                                                                                        tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACt                                                                                                                 >  1:953417/1‑43 (MQ=255)
                                                                                                                                        tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACt                                                                                                                 <  2:953417/43‑1 (MQ=255)
                                                                                                                                        tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCGGACGCGACTTATCAGGCCTAACGGGTGCCCTGAACTGTAGGTCg       >  2:170191/1‑149 (MQ=14)
                                                                                                                                          aTGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa     <  1:1257171/149‑1 (MQ=255)
                                                                                                                                             cGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATaa  <  2:1396517/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TCGACACCATGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAA  >  NC_000913_3_pae_tpiA/349472‑349761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: