New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 225456 =10 (0.080)25 (0.220) 21/242 9.7 72.9% noncoding (76/77 nt) ileV tRNA‑Ile
?NC_000913_3_pae_tpiA = 3427056 NA (NA)intergenic (‑23/+20) alaU/ileU tRNA‑Ala/tRNA‑Ile
Rejected: Coverage evenness skew score above cutoff.

ACACTCTGAAGTATTTTTTATTTAACCACTACAGAGATGGTGGAGCTATGCGGGATCGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/225612‑225456
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcaaatttgAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGG  <  NC_000913_3_pae_tpiA/3427056‑3426910
                                                                                                                                                                                                                                                                                                                
ACACTCTGAAGTATTTTTTATTTAACCACTACAGAGATGGTGGAGCTATGCGGGATCGAACCGCAGACCTCCTGCGTGCAACGCAGGCGCTCTCCCAGCTGGGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGC                                                                                                                                                            >  1:1150407/1‑150
ACACTCTGAAGTATTTTTTATTTAACCACTACAGAGATGGTGGAGCTATGCGGGATCGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGC                                                                                                                                                            >  1:1150318/1‑150
    TCTGAAGTATTTTTTATTTAACCACTACAGAGATGGTGGAGCTATGCGGGATCGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAAT                                                                                                                                                        >  1:1392685/1‑150
      TGAAGTATTTTTTATTTAACCACTACAGAGATGGTGGAGCTATGCGGGATCGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCACCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTT                                                                                                                                                      >  1:1249878/1‑150
                           ACTACAGAGATGGTGGAGCTATGCGGGATCGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTG                                                                                                                                                             <  4:292390/122‑1
                           ACTACAGAGATGGTGGAGCTATGCGGGATCGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTG                                                                                                                                                             >  3:292390/1‑122
                                      GGTGGAGCTATGCGGGATCGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTAT                                                                                                                      <  4:150800/150‑1
                                                                AGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATT                                                                                                                                                       >  4:756095/1‑91
                                                                AGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATT                                                                                                                                                       <  3:756095/91‑1
                                                                     TCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTT                                                                                                                                                      <  1:551581/87‑1
                                                                     TCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTT                                                                                                                                                      >  2:551581/1‑87
                                                                              CAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTT                                                                                                                                                      >  4:888196/1‑78
                                                                              CAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTT                                                                                                                                                      <  3:888196/78‑1
                                                                                            TCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTT                                                                       >  1:397324/1‑143
                                                                                            TCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTT                                                                       <  2:397324/143‑1
                                                                                              CCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGGAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAG                                                                                                        >  4:77604/1‑108
                                                                                              CCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGGAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAG                                                                                                        <  3:77604/108‑1
                                                                                                        TATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGTGCGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCAC                                                    >  4:78962/1‑150
                                                                                                                 ATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGT                                           >  1:367519/1‑150
                                                                                                                      ATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCC                                                                                                  <  1:954765/90‑1
                                                                                                                      ATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCC                                                                                                  >  2:954765/1‑90
                                                                                                                         TAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGC                                                                                                     <  1:347003/84‑1
                                                                                                                         TAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGC                                                                                                     >  2:347003/1‑84
                                                                                                                           GTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGGAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGC                                                                                               >  1:901440/1‑88
                                                                                                                           GTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGGAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGC                                                                                               <  2:901440/88‑1
                                                                                                                                     CTTCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATAC                       >  2:1413440/1‑150
                                                                                                                                      TTCAAATTTGCCGTGCAAATTTGGAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTT                                                                       <  1:174200/101‑1
                                                                                                                                      TTCAAATTTGCCGTGCAAATTTGGAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTT                                                                       >  2:174200/1‑101
                                                                                                                                      TTCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACT                      >  2:49326/1‑150
                                                                                                                                      TTCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAG                                                                                 <  1:962067/91‑1
                                                                                                                                      TTCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAG                                                                                 >  2:962067/1‑91
                                                                                                                                       TCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCG                                                                          <  2:458032/97‑1
                                                                                                                                       TCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCG                                                                          >  1:458032/1‑97
                                                                                                                                       TCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCT                                                                                                 >  4:118823/1‑74
                                                                                                                                       TCAAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCT                                                                                                 <  3:118823/74‑1
                                                                                                                                         AAATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCA                   >  2:240923/1‑150
                                                                                                                                          AATTTGCCGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAG                  >  2:286191/1‑150
                                                                                                                                                    GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTCTGCACGCAGGAGGTCTGCGGTTCGATCCCGC                                                              <  1:1409542/96‑1
                                                                                                                                                    GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTCTGCACGCAGGAGGTCTGCGGTTCGATCCCGC                                                              >  2:1409542/1‑96
                                                                                                                                                     CAAATTTGAAGAGGTTTTAACTACATGTTATGGGGTTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGC       >  1:735931/1‑150
                                                                                                                                                     CAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGC       >  2:473804/1‑150
                                                                                                                                                      AAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCA      >  3:767192/1‑150
                                                                                                                                                      AAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCA      <  2:1299894/150‑1
                                                                                                                                                      AAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTC                                                                      >  2:24/1‑86
                                                                                                                                                      AAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTC                                                                      <  1:24/86‑1
                                                                                                                                                        ATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAA    <  3:384016/150‑1
                                                                                                                                                        ATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGT                                        <  3:459874/114‑1
                                                                                                                                                        ATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGT                                        >  4:459874/1‑114
                                                                                                                                                         TTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGT             >  2:641339/1‑140
                                                                                                                                                         TTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGT             <  1:641339/140‑1
                                                                                                                                                          TTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGG  <  3:264917/150‑1
                                                                                                                                                                                                                                                                                                                
ACACTCTGAAGTATTTTTTATTTAACCACTACAGAGATGGTGGAGCTATGCGGGATCGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/225612‑225456
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcaaatttgAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGG  <  NC_000913_3_pae_tpiA/3427056‑3426910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.