Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913_3_pae_tpiA | 4,187,130 | A→G | D622G (GAC→GGC) | rpoC → | RNA polymerase, beta prime subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913_3_pae_tpiA | 4,187,130 | 0 | A | G | 97.4% | 389.9 / ‑4.2 | 114 | D622G (GAC→GGC) | rpoC | RNA polymerase, beta prime subunit |
Reads supporting (aligned to +/- strand): ref base A (0/1); major base G (45/65); minor base T (3/0); total (48/66) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.39e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 114 total aligned reads displayed.
TGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTG > NC_000913_3_pae_tpiA/4186984‑4187276 | tGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCa > 2:696410/1‑150 (MQ=255) gTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTCTTTTTGCGGGCCAg > 1:785678/1‑150 (MQ=255) ttGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGa < 3:98987/150‑1 (MQ=255) gCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCa < 2:1325320/150‑1 (MQ=255) ccGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCAt > 1:270722/1‑150 (MQ=255) cGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATg < 2:21452/150‑1 (MQ=255) tGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTa > 2:855279/1‑150 (MQ=255) ttCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCg > 2:701696/1‑150 (MQ=255) tgtgGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCt > 4:164213/1‑150 (MQ=255) tgGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTc < 1:701696/150‑1 (MQ=255) gatgatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGc < 3:462641/150‑1 (MQ=255) atgatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGcc < 3:754610/150‑1 (MQ=255) atgatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGcc < 2:270722/150‑1 (MQ=255) tgatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCt < 2:721407/150‑1 (MQ=255) ttGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATg > 2:179326/1‑150 (MQ=255) ttGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCACGGCCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATg > 1:426946/1‑150 (MQ=255) gTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCa < 1:1239780/150‑1 (MQ=255) gTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCa < 1:1239770/150‑1 (MQ=255) aCCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAgc > 4:239291/1‑150 (MQ=255) aCCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAgc > 4:239352/1‑150 (MQ=255) aCCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAgc < 1:696410/150‑1 (MQ=255) gAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGcgcg > 1:1069777/1‑150 (MQ=255) tCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTgg < 1:211709/150‑1 (MQ=255) tCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTgg > 2:1203406/1‑150 (MQ=255) cTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGt > 4:455179/1‑150 (MQ=255) cTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGt < 1:1011242/150‑1 (MQ=255) gCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGTCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGc < 2:992323/150‑1 (MQ=255) gCCTCACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTa > 3:654126/1‑117 (MQ=255) gCCTCACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTa > 3:654010/1‑117 (MQ=255) gCCTCACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTa < 4:654010/117‑1 (MQ=255) tCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGtt > 2:70883/1‑150 (MQ=255) tCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTACAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGtt > 4:851328/1‑150 (MQ=255) ccATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGGTCTGGTGCATCTGTTg < 2:737062/150‑1 (MQ=255) cATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTg > 1:1355865/1‑137 (MQ=255) cATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTg < 2:1355865/137‑1 (MQ=255) aTCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGGCCGTTATTTTTGCGGGCCAGAACATGCACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGt > 1:1149718/1‑150 (MQ=255) aTCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGt > 1:78138/1‑150 (MQ=255) cGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGc > 1:150114/1‑117 (MQ=255) cAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGa < 4:502221/150‑1 (MQ=255) cAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCCGGTGCATCTGTTGGTATCGa < 1:663188/150‑1 (MQ=255) aaCCAGGCGCTTGGTAAAAAAGCAAACTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGAt < 4:590651/150‑1 (MQ=255) ccAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGa < 1:70883/150‑1 (MQ=255) cAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGAc > 4:698360/1‑150 (MQ=255) gcgcTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATg < 3:239352/150‑1 (MQ=255) gcgcTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATg < 3:239291/150‑1 (MQ=255) gcTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATgg < 1:466440/149‑1 (MQ=255) gcTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATgg > 2:466440/1‑149 (MQ=255) aaaaaaGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCg < 1:921660/150‑1 (MQ=255) aaaaGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGga < 2:744215/150‑1 (MQ=255) cAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGagaaga < 4:264190/150‑1 (MQ=255) cAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGagaaga > 1:1340222/1‑150 (MQ=255) tctcCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAac > 4:603429/1‑150 (MQ=255) ctcCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAaca > 3:49459/1‑150 (MQ=255) tcCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAacac > 4:485050/1‑150 (MQ=255) tcCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAacac < 1:1203406/150‑1 (MQ=255) ccAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACg < 2:1072816/150‑1 (MQ=255) cAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTgg > 1:632786/1‑102 (MQ=255) cAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGa < 2:1441314/150‑1 (MQ=255) aaaaTGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGaa < 1:207123/150‑1 (MQ=255) aaaTGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGaaa > 2:603443/1‑150 (MQ=255) aaaTGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGaaa < 3:29378/150‑1 (MQ=255) aacgCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATg > 1:530547/4‑86 (MQ=255) aacgCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATg < 2:530547/83‑1 (MQ=255) aTGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAatc < 4:335524/150‑1 (MQ=255) tGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCAtct > 3:737084/1‑150 (MQ=255) tGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCAtct < 4:737084/150‑1 (MQ=255) gAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGtt > 3:664065/1‑103 (MQ=255) gAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGtt < 4:664065/103‑1 (MQ=255) aaCACCTGCTACCGCATTCTCGGTCTGAAAGCGACCGTTATTTTTGCGTGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTcc < 1:331300/150‑1 (MQ=255) aaCACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCAtcttc > 4:827476/1‑148 (MQ=255) acacCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCg < 3:256221/150‑1 (MQ=255) cacCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGa < 1:603443/150‑1 (MQ=255) cacCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGa < 3:782903/150‑1 (MQ=255) cTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgc < 2:785678/150‑1 (MQ=255) cTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTTTTTTTTTTTTCATGTACACCGGCTTCACCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGCTCACCTGGTCATCCCGGCGAAGAAACACGTAATCATCTCCGAGgcata > 4:797491/1‑148 (MQ=255) cTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcaga > 2:1443843/1‑150 (MQ=255) aCCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaag < 2:1069777/150‑1 (MQ=255) ccGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTGTCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaagc > 3:288073/1‑150 (MQ=255) cGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCCCCGAGgcagaagca > 1:1429826/1‑150 (MQ=255) cATTCTCGGTCTGAAACCGACCGTTATTTTTTCGTGCCTTATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaagcaga > 2:1023779/1‑150 (MQ=255) aTTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaagcagaa < 3:501240/150‑1 (MQ=255) tctcGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGTTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGt > 1:396765/1‑150 (MQ=255) cGGTCTGAAGCCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCAACCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGc < 4:861559/150‑1 (MQ=255) gTCTGAAACCGACCGTTATTTTTGCGGGCCAGATc < 2:749704/35‑1 (MQ=255) gTCTGAAACCGACCGTTATTTTTGCGGGCCAGATc > 1:749704/1‑35 (MQ=255) tGAAACCGACCGTTATTTTTTTTTTTCTTATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCAATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGaaa > 4:408349/1‑150 (MQ=255) tGAAACCGACCGTTATTTTTTTTTTCCATATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGaaa > 2:748248/1‑150 (MQ=255) tGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCt < 1:1005311/146‑1 (MQ=255) tGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCt > 2:1005311/1‑146 (MQ=255) tGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGaaa < 3:152196/150‑1 (MQ=255) tGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGaaa > 2:1449650/1‑150 (MQ=255) aaCCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGAAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTc > 2:209377/1‑150 (MQ=255) accgaccgTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCa < 3:698360/150‑1 (MQ=255) ccgaccgTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAg < 1:179326/150‑1 (MQ=255) ccgaccgTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCACCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAg < 4:49459/150‑1 (MQ=255) ttATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGt < 1:855279/150‑1 (MQ=255) ttGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAgt < 3:455179/150‑1 (MQ=255) ttGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAgt < 3:454744/150‑1 (MQ=255) gCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCt < 2:1319041/126‑1 (MQ=255) cGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAgtctg < 1:1290895/150‑1 (MQ=255) | TGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTG > NC_000913_3_pae_tpiA/4186984‑4187276 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |