Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 1,532,199 C→A V128V (GTC→GTA ydcC → H repeat‑associated putative transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,532,1990CA93.3% 310.1 / 17.5 120V128V (GTC→GTAydcCH repeat‑associated putative transposase
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base A (58/54);  total (62/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 120 total aligned reads displayed.

GTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAAT  >  NC_000913_3_pae_tpiA/1532054‑1532318
                                                                                                                                                 |                                                                                                                       
gTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTa                                                                                                                     <  2:671270/150‑1 (MQ=25)
       tCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGtt                                                                                                              >  1:745729/1‑150 (MQ=25)
          tGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTtctc                                                                                                           <  4:544325/150‑1 (MQ=25)
            cAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTcaa                                                                                                         <  3:265532/150‑1 (MQ=25)
            cAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTcaa                                                                                                         >  3:91677/1‑150 (MQ=25)
            cAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTcaa                                                                                                         >  4:41501/1‑150 (MQ=25)
             aaaaTTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTcaac                                                                                                        >  1:1021502/1‑150 (MQ=25)
             aaaaTTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTcaac                                                                                                        >  4:439429/1‑150 (MQ=25)
              aaaTTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTcaaca                                                                                                       >  1:119333/1‑150 (MQ=35)
              aaaTTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTcaaca                                                                                                       >  1:119332/1‑150 (MQ=35)
                     aCGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGcaca                                                                                                >  4:180621/1‑150 (MQ=25)
                       gAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGt                                                                                              <  1:871827/150‑1 (MQ=35)
                       gAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGt                                                                                              >  1:781115/1‑150 (MQ=25)
                        aGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTc                                                                                             >  1:1157366/1‑150 (MQ=25)
                        aGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTc                                                                                             <  2:1021502/150‑1 (MQ=25)
                           gCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATGCGTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTgg                                                                                          >  3:580458/1‑150 (MQ=255)
                           gCTTTATTAACTGGAGGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTgg                                                                                          <  3:767454/150‑1 (MQ=255)
                            cTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAg                                                                                                                    >  2:63674/1‑123 (MQ=37)
                             tttATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTc                                                                                             <  2:464192/145‑1 (MQ=25)
                             tttATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTc                                                                                        <  1:180554/150‑1 (MQ=25)
                                attaACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATc                                                                                     <  1:688984/150‑1 (MQ=25)
                                 ttaACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCg                                                                                    <  2:781115/150‑1 (MQ=25)
                                 ttaACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCg                                                                                    <  1:1281607/150‑1 (MQ=25)
                                 ttaACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCg                                                                                    <  2:731831/150‑1 (MQ=25)
                                 ttaACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCg                                                                                    <  1:746318/150‑1 (MQ=25)
                                 ttaACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCg                                                                                    >  3:347946/1‑150 (MQ=25)
                                  taACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCgg                                                                                   >  4:215107/1‑150 (MQ=25)
                                   aaCTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATAGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGa                                                                                  <  3:278254/150‑1 (MQ=255)
                                        gATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGAt                                                                             <  2:889044/150‑1 (MQ=25)
                                        gATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGAt                                                                             >  4:698563/1‑150 (MQ=25)
                                            cGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTa                                                                                                                     >  3:544054/1‑106 (MQ=255)
                                             gTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGa                                                                        >  1:1121174/1‑150 (MQ=25)
                                             gTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGa                                                                        >  1:387307/1‑150 (MQ=25)
                                              tGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGAc                                                                       <  1:512743/150‑1 (MQ=25)
                                                  tGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGAt                                                                   >  3:820230/1‑150 (MQ=25)
                                                  tGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGAt                                                                   >  1:1369003/1‑150 (MQ=25)
                                                     cATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATgag                                                                <  1:278019/150‑1 (MQ=25)
                                                      aTTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATgaga                                                               <  4:523489/150‑1 (MQ=25)
                                                       ttcttcAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATg                                                                                                    >  3:302015/1‑112 (MQ=255)
                                                       ttcttcAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATg                                                                                                    <  4:302015/112‑1 (MQ=255)
                                                       ttcttcAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGaa                                                              >  3:817650/1‑150 (MQ=25)
                                                        tcttcAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGaaa                                                             <  3:89159/150‑1 (MQ=25)
                                                         cttcAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTcaa                                                                                                         >  1:861599/1‑105 (MQ=255)
                                                         cttcAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTcaa                                                                                                         <  2:861599/105‑1 (MQ=255)
                                                            cAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTa                                                         <  2:119333/150‑1 (MQ=34)
                                                            cAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTa                                                         <  2:119332/150‑1 (MQ=35)
                                                             aGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTaa                                                        <  1:203373/150‑1 (MQ=25)
                                                             aGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTaa                                                        >  1:1019697/1‑150 (MQ=25)
                                                             aGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTaa                                                        <  1:455037/150‑1 (MQ=25)
                                                             aGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGAAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTaa                                                        >  2:414791/1‑150 (MQ=21)
                                                              gatgatAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGt                                                                                              <  3:702728/111‑1 (MQ=255)
                                                              gatgatAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGt                                                                                              >  4:702728/1‑111 (MQ=255)
                                                                  atAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATgaga                                                   >  2:483750/1‑150 (MQ=25)
                                                                   tAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGAt                                                  >  1:1404890/1‑150 (MQ=25)
                                                                    aaaGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGAtt                                                 >  1:781155/1‑150 (MQ=25)
                                                                    aaaGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGAtt                                                 >  3:43143/1‑150 (MQ=25)
                                                                      aGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTAc                                               >  2:1212242/1‑150 (MQ=35)
                                                                      aGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTAc                                               <  1:483750/150‑1 (MQ=25)
                                                                      aGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTAc                                               <  2:716376/150‑1 (MQ=25)
                                                                        aCGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGc                                                                                                                 >  2:137563/1‑82 (MQ=255)
                                                                        aCGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGc                                                                                                                 <  1:137563/82‑1 (MQ=255)
                                                                          gTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATga                                                     <  2:634181/140‑1 (MQ=21)
                                                                          gTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATga                                                     >  1:634181/1‑140 (MQ=21)
                                                                          gTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCt                                           >  1:1274472/1‑150 (MQ=25)
                                                                              ttGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATcc                                       >  1:476640/1‑150 (MQ=25)
                                                                                 cAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAg                                    >  3:49138/1‑150 (MQ=25)
                                                                                  aaTTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCg                                                                                    >  1:118873/1‑101 (MQ=255)
                                                                                  aaTTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCg                                                                                    <  2:118873/101‑1 (MQ=255)
                                                                                   aTTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGt                                                                                                               <  2:1353969/73‑1 (MQ=255)
                                                                                   aTTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGaa                                  <  2:387307/150‑1 (MQ=25)
                                                                                    ttGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTcaa                                                                                                         <  2:918927/78‑1 (MQ=255)
                                                                                    ttGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTcaa                                                                                                         >  1:918927/1‑78 (MQ=255)
                                                                                     tGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAg                                    >  1:557994/1‑146 (MQ=25)
                                                                                       aTGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTg                                                                                                                  >  3:240741/1‑66 (MQ=255)
                                                                                       aTGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTg                                                                                                                  <  4:240741/66‑1 (MQ=255)
                                                                                       aTGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTtc                                                                                                             <  4:32007/71‑1 (MQ=255)
                                                                                         ggAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAg                                                                                                                    >  2:1389235/1‑62 (MQ=255)
                                                                                         ggAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAg                                                                                                                    <  1:1389235/62‑1 (MQ=255)
                                                                                            aaaaCGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAAc                         <  4:817650/150‑1 (MQ=25)
                                                                                             aaaCGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACa                        <  2:174447/150‑1 (MQ=21)
                                                                                                 gCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGGGCGATTCATGTAATTAGTGCGTTCTCAACAATCCACAGTCTGGTCATCGGACAGATCAACACGGATGAGAAATCTAATGAGATTACAACTATCCCAGAACTTCTTATCATGCt                    <  2:238208/150‑1 (MQ=11)
                                                                                                   tCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTgg                  <  2:781155/150‑1 (MQ=25)
                                                                                                     gggCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGGAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGat                <  2:279100/149‑1 (MQ=25)
                                                                                                     cGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTAt                                         >  4:887818/1‑125 (MQ=37)
                                                                                                     cGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTAt                                         <  3:887818/125‑1 (MQ=37)
                                                                                                           tCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTaaa          <  4:820230/150‑1 (MQ=17)
                                                                                                           tCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTaaa          <  3:215107/150‑1 (MQ=17)
                                                                                                             ttATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAg                                    >  3:776745/1‑122 (MQ=18)
                                                                                                             ttATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAgg        >  4:711411/1‑150 (MQ=17)
                                                                                                             ttATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAgg        >  4:807085/1‑150 (MQ=17)
                                                                                                             ttATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAgg        >  4:807209/1‑150 (MQ=17)
                                                                                                              tATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAAt                                                                                                                       <  2:1058665/38‑1 (MQ=255)
                                                                                                                tGATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGaaa     <  4:43143/150‑1 (MQ=17)
                                                                                                                 gATAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGaaaa    <  1:1212242/150‑1 (MQ=32)
                                                                                                                 gATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACa                        <  2:1191138/130‑1 (MQ=17)
                                                                                                                 gATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGaaaa    >  1:1407731/1‑150 (MQ=17)
                                                                                                                 gATAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGaaaa    >  4:630241/1‑150 (MQ=17)
                                                                                                                  aTAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTaaa          <  4:476954/143‑1 (MQ=14)
                                                                                                                  aTAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTaaa          >  3:476954/1‑143 (MQ=14)
                                                                                                                  aTAAGAGTCGCCGCAGGGGAGCGATTCATGTAATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGaaaaa   >  3:508919/1‑150 (MQ=14)
                                                                                                                                                 |                                                                                                                       
GTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGACAGATCAAGACGGATGAGAAATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAAT  >  NC_000913_3_pae_tpiA/1532054‑1532318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: