New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 417181278 (0.770)18 (0.190) 17/224 8.1 20.5% intergenic (+140/‑161) rrfB/murB 5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding
?NC_000913_3_pae_tpiA = 4171817 67 (0.710)intergenic (+145/‑156) rrfB/murB 5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding
Rejected: Coverage evenness skew score above cutoff.

AGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/4171656‑4171812
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tggcgggcGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGC  <  NC_000913_3_pae_tpiA/4171817‑4171669
                                                                                                                                                                                                                                                                                                                       
AGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGACCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GT                                                                                                                                                              >  2:840158/1‑150
  GGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGG                                                                                                                                                            >  2:1000347/1‑150
       TGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGC                                                                                                                                                       >  1:1122955/1‑150
           AGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGC                                                                                                                                                           >  1:1205189/1‑142
           AGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGC                                                                                                                                                           <  2:1205189/142‑1
                        AACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGC                                                                                                                                                       <  2:698/133‑1
                        AACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGC                                                                                                                                                       >  1:698/1‑133
                        AACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGCGTCCTGCCCGCCAC                                                                                                                                         <  4:552358/147‑1
                                              CTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGCGTCCTGCCCGCCA                                                                                                                                          <  2:465902/124‑1
                                              CTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGCGTCCTGCCCGCCA                                                                                                                                          >  1:465902/1‑124
                                                                      TTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGCGT                                                                                                                                                     <  1:1118646/89‑1
                                                                      TTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGCGT                                                                                                                                                     >  2:1118646/1‑89
                                                                            GGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGCGTCCTGCCCGCCA                                                                                                                                          >  1:676086/1‑94
                                                                            GGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGCGTCCTGCCCGCCA                                                                                                                                          <  2:676086/94‑1
                                                                             GTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCG                                                                                                                              <  2:70746/105‑1
                                                                             GTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCG                                                                                                                              >  1:70746/1‑105
                                                                              TGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCTGGCAGTTT‑‑ATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTG                                                                                                                           >  1:913582/1‑110
                                                                              TGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCTGGCAG‑‑TTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTG                                                                                                                           <  2:913582/110‑1
                                                                                   GCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC                                                                                                                     >  1:289873/1‑108
                                                                                   GCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC                                                                                                                     <  2:289873/108‑1
                                                                                                 GACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAAT                                                                                                          <  1:385546/105‑1
                                                                                                 GACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAAT                                                                                                          >  2:385546/1‑105
                                                                                                          GCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGG                                                                                                                                                        <  3:442793/50‑1
                                                                                                          GCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGG                                                                                                                                                        >  4:442793/1‑50
                                                                                                                                              CGGAG‑‑G‑‑GTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGAT                                                   <  3:553917/115‑1
                                                                                                                                              CGGAG‑‑G‑‑GTGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGAT                                                   >  4:553917/1‑115
                                                                                                                                                GAG‑‑G‑‑GTGGCGGGCGTCATGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTGCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGCAAGGCCCAGTCTTTCGACTGAGCCTATCGg              >  2:1168009/1‑149
                                                                                                                                                          TGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTAT         >  2:1122955/1‑150
                                                                                                                                                          TGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTAC                                                                                  >  2:625866/1‑77
                                                                                                                                                          TGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTAC                                                                                  <  1:625866/77‑1
                                                                                                                                                          TGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC                                                                                                                       >  4:387614/1‑40
                                                                                                                                                          TGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC                                                                                                                       <  3:387614/40‑1
                                                                                                                                                           GGCGGGCGTCCTGCCCGCCCCACTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGAT                                                                                           <  2:391207/67‑1
                                                                                                                                                           GGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGAT                                                   >  3:91522/1‑107
                                                                                                                                                           GGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGAT                                                   <  4:91522/107‑1
                                                                                                                                                           GGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGAT                                                                                           >  1:391207/1‑67
                                                                                                                                                            GCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTT       <  4:197245/150‑1
                                                                                                                                                            GCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTT       >  2:350757/1‑150
                                                                                                                                                            GCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTT            <  3:168056/145‑1
                                                                                                                                                            GCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTT            >  4:168056/1‑145
                                                                                                                                                            GCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAA                                               >  4:306053/1‑110
                                                                                                                                                            GCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAA                                               <  3:306053/110‑1
                                                                                                                                                            GCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAC                                                                  >  1:303164/1‑91
                                                                                                                                                            GCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAC                                                                  <  2:303164/91‑1
                                                                                                                                                             CGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTG      <  1:979662/150‑1
                                                                                                                                                             CGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCG                                                                       <  3:724150/85‑1
                                                                                                                                                             CGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCG                                                                       >  4:724150/1‑85
                                                                                                                                                             CGGGCGTACTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAA                                                           <  4:217503/97‑1
                                                                                                                                                             CGGGCGTACTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAA                                                           >  3:217503/1‑97
                                                                                                                                                              GGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGa                                                                                       >  2:1178884/1‑67
                                                                                                                                                              GGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGa                                                                                       <  1:1178884/68‑2
                                                                                                                                                              GGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTT             <  1:818973/142‑1
                                                                                                                                                              GGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTT             >  2:818973/1‑142
                                                                                                                                                                GCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATC                                                                                                         >  1:778211/1‑48
                                                                                                                                                                GCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATC                                                                                                         <  2:778211/48‑1
                                                                                                                                                                 CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAAAAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGC  <  4:5433/150‑1
                                                                                                                                                                                                                                                                                                                       
AGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAG‑‑G‑‑GTGGCGGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/4171656‑4171812
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tggcgggcGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGC  <  NC_000913_3_pae_tpiA/4171817‑4171669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.