New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 3570877	167 (0.950)	23 (0.140)	19/250	15.1	12.6%	coding (443/1974 nt)	glgX	glycogen debranching enzyme
	?	NC_000913_3_pae_tpiA	= 3570891	159 (0.950)					coding (429/1974 nt)	glgX	glycogen debranching enzyme
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/3570724‑3570877 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgccccGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTATTTGAAACAATTGGGC  <  NC_000913_3_pae_tpiA/3570891‑3570744                                                                                                                                                                                                                                                                                                                AGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTG                                                                                                                                                          >  3:574738/1‑150   CAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCTGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCC                                                                                                                                                        <  2:2064710/150‑1   CAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCC                                                                                                                                                        >  1:345297/1‑150          AGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGC                                                                                                                                                 >  1:1495803/1‑150                      CCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGGCCAAGCACGTTGATCCTTTGACATGGGCTTCATAAATGATGGTGATGCCCCGCCGCGCACGCCGTGGG                                                                                                                                     >  3:695830/1‑150                        CAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTGTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGC                                                                                                                                   <  2:1198555/150‑1                         AATTGTTTCAAATAGTTGATCATCACCGGATGCTCGTGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCA                                                                                                                                  <  3:47738/150‑1                         AATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCA                                                                                                                                  <  3:328580/150‑1                          ATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCAG                                                                                                                                 <  2:1699742/150‑1                                    ATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACTGCGATCTCCGGGTGCAAGTAAGCTAATACTTTGTCCTGGGTTTCATTAATGATGGTGCTTCCCCGCCTCGCACGCCGTGGGGCAGCACCATCATT                                                                                                                       >  1:927104/1‑150                                    ATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGATGCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATT                                                                                                                       <  4:1214751/150‑1                                      AGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTA                                                                                                                     <  1:1912470/150‑1                                      AGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTA                                                                                                                     <  2:1495803/150‑1                                      AGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTA                                                                                                                     <  1:1912460/150‑1                                       GTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCAGCACC                                                                                                                             >  1:1790925/1‑141                                       GTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCAGCACC                                                                                                                             <  2:1790925/141‑1                                              ATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCC                                                                                                             <  1:836649/150‑1                                               TCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGG                                                                                                                                    <  2:1416308/126‑1                                               TCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGG                                                                                                                                    >  1:1416308/1‑126                                                       TGCCCGAGGGCATAAGGAGTTCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAG                                                                                                    <  2:927104/150‑1                                                                        AGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCAC                                                                                   <  1:1824297/150‑1                                                                            CCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCAGCACCATC                                                                                                                          >  1:460624/1‑107                                                                            CCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCAGCACCATC                                                                                                                          <  2:460624/107‑1                                                                             CACGGATCGCGACCGGGATGTGCGGGTGCAAGTGCGTTAATCCTTTGACAGGGGCCGCATAAATGATGGTCATGCCCCACCGCGCACGCCGGGGGGCAAAGCCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGA                                                                              <  2:1776722/150‑1                                                                                     TCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCA                                                                                                                                  <  2:985286/90‑1                                                                                     TCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCA                                                                                                                                  >  1:985286/1‑90                                                                                                                                                     TGCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTATTTGAAACAATT      <  2:65212/150‑1                                                                                                                                                      GCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTATTTGAAACAATTG     >  4:300839/1‑150                                                                                                                                                      GCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTT                                                    <  2:304334/103‑1                                                                                                                                                      GCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTT                                                    >  1:304334/1‑103                                                                                                                                                       CCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTATTTGAAACAATTGG    <  2:1060262/150‑1                                                                                                                                                       CCCCGCCGCGCACGCCGTAGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTATTTGAAACAATTGG    >  2:452582/1‑150                                                                                                                                                        CCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTATTTGAAACAATTGGG   <  3:29745/150‑1                                                                                                                                                        CCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTATTTGAAACAATTGGG   <  2:1561817/150‑1                                                                                                                                                         CCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATATCAACGGCTTAACGTACTTGCACCCGGCGATCTCGGTCGAGATCCGTGGCCCTTACAAAGCCCTCGGGCCTCCGGTCATGATCCCCTATTTCAAACAGTTGGGC  >  3:126502/1‑150                                                                                                                                                          CGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTATT                <  2:869949/135‑1                                                                                                                                                          CGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTATT                >  1:869949/1‑135                                                                                                                                                          CGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGT                                                                                                         >  3:290986/1‑46                                                                                                                                                          CGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGT                                                                                                         <  4:290986/46‑1                                                                                                                                                                                                                                                                                                                AGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/3570724‑3570877 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgccccGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTATTTGAAACAATTGGGC  <  NC_000913_3_pae_tpiA/3570891‑3570744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.