Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	1,756,693	C→A	C332* (TGC→TGA)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	1,756,693	0	C	A	100.0%	230.7 / NA	65	C332* (TGC→TGA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/30);  total (35/30)

AAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGC  >  NC_000913_3_pae_tpiA/1756554‑1756837                                                                                                                                            |                                                                                                                                                 aaaaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGCCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGGTTCTATCATGGCGACCATCTGAGAACGTaccg                                                                                                                                        >  4:454243/1‑150 (MQ=255)      ccACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACcgc                                                                                                                                   >  4:268361/1‑150 (MQ=255)      ccACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACcgc                                                                                                                                   >  4:17486/1‑150 (MQ=255)         cGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGtg                                                                                                                                <  1:394878/150‑1 (MQ=255)          gCCCGCCGCGCGCAGCAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGtga                                                                                                                               <  4:2575/150‑1 (MQ=255)             cGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGtgatga                                                                                                                            >  1:196694/1‑150 (MQ=255)             cGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGtgatga                                                                                                                            <  2:196694/150‑1 (MQ=255)                cTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACa                                                                                                                         <  3:348370/150‑1 (MQ=255)                 tCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAg                                                                                                                        <  2:723275/150‑1 (MQ=255)                     gcAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGcc                                                                                                                      <  2:152551/148‑1 (MQ=255)                     gcAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGcc                                                                                                                      >  1:152551/1‑148 (MQ=255)                       aGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGtct                                                                                                                  >  3:532067/1‑150 (MQ=255)                                  aCGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGt                                                                                                                                 >  2:850451/1‑124 (MQ=255)                                  aCGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGt                                                                                                                                 <  1:850451/124‑1 (MQ=255)                                    gTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAAt                                                                                                     <  2:258828/150‑1 (MQ=255)                                         aaaCGCCATCCTCGACGTTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGtga                                                                                                                               >  2:578790/1‑119 (MQ=255)                                         aaaCGCCATCCTCGACGTTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGtga                                                                                                                               <  1:578790/119‑1 (MQ=255)                                          aaCGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTcaa                                                                                                         >  3:214399/1‑140 (MQ=255)                                          aaCGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTcaa                                                                                                         <  4:214399/140‑1 (MQ=255)                                             gCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGt                                                                                            >  1:840311/1‑150 (MQ=255)                                                 tCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAAc                                                                                        <  1:538958/150‑1 (MQ=255)                                                            aCTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTAcc                                                                             >  3:513248/1‑150 (MQ=255)                                                                  gCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCg                                                                       <  3:268361/150‑1 (MQ=255)                                                                    aGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTGTCATGGCGAGCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGt                                                                     <  1:455611/150‑1 (MQ=255)                                                                     gTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTg                                                                                      >  2:821415/1‑132 (MQ=255)                                                                     gTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTg                                                                                      <  1:821415/132‑1 (MQ=255)                                                                       gATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATg                                                                  >  3:505148/1‑150 (MQ=255)                                                                           cTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgt                                                              >  4:367879/1‑150 (MQ=255)                                                                            tGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtg                                                             <  2:473344/150‑1 (MQ=255)                                                                             gTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTca                                                                                                          <  2:585319/104‑1 (MQ=255)                                                                             gTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTca                                                                                                          >  1:585319/1‑104 (MQ=255)                                                                              tCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtggt                                                           >  1:910641/1‑150 (MQ=255)                                                                               cTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtggtg                                                          <  4:397412/150‑1 (MQ=255)                                                                                   tGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTa                                                                                                                                           >  4:245381/1‑65 (MQ=255)                                                                                   tGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTa                                                                                                                                           <  3:245381/65‑1 (MQ=255)                                                                                    gAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGCCAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGtt                                                     <  4:532067/150‑1 (MQ=255)                                                                                    gAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGtt                                                     >  1:772660/1‑150 (MQ=255)                                                                                     aaTCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTg                                                    >  4:537075/1‑150 (MQ=255)                                                                                      aTCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCTGTTGa                                                   <  3:17486/150‑1 (MQ=255)                                                                                         cGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGGTCAACAATGACAACCGTAAACTGCGCATTACCGAAGAGGGATGCCGGGGTGGCGTTGaaaa                                                >  2:699876/1‑149 (MQ=255)                                                                                          gCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCa                                                                                  <  4:160021/115‑1 (MQ=255)                                                                                          gCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCa                                                                                  >  3:160021/1‑115 (MQ=255)                                                                                          gCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAAct                                               >  3:185584/1‑150 (MQ=255)                                                                                          gCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAAct                                               >  1:22078/1‑150 (MQ=255)                                                                                              aaGGGAAATACCCGCTGGACGCGGTTTCTATCATGGCGACCACCTGAGAACGTACCGACCGCGTGATGAACGGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGACGCGGTATGCCGTGGTGCCGTTGAAActgctg                                           <  4:550962/150‑1 (MQ=255)                                                                                                 gTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGaaa                                        >  1:891070/1‑150 (MQ=255)                                                                                                    aaTACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAAc                                     <  1:699876/150‑1 (MQ=255)                                                                                                    aaTACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAAc                                     <  3:537075/150‑1 (MQ=255)                                                                                                          cGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGATCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATg                               >  1:254726/1‑150 (MQ=255)                                                                                                                 aGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAActgc                                             <  1:645509/129‑1 (MQ=255)                                                                                                                 aGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAActgc                                             >  2:645509/1‑129 (MQ=255)                                                                                                                     gTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACACCCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAActgc                                             <  1:484328/125‑1 (MQ=255)                                                                                                                     gTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAActgc                                             >  2:484328/1‑125 (MQ=255)                                                                                                                     gTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATc                    >  3:110384/1‑150 (MQ=255)                                                                                                                           aTCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtg                                                             >  1:308945/1‑103 (MQ=255)                                                                                                                           aTCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtg                                                             <  2:308945/103‑1 (MQ=255)                                                                                                                           aTCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACt                                    <  3:271998/128‑1 (MQ=255)                                                                                                                           aTCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACt                                    >  4:271998/1‑128 (MQ=255)                                                                                                                           aTCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGtt              >  3:197567/1‑150 (MQ=255)                                                                                                                            tCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGAc                                                                                                  >  4:173725/1‑65 (MQ=255)                                                                                                                            tCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGAc                                                                                                  <  3:173725/65‑1 (MQ=255)                                                                                                                            tCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTg             >  1:699499/1‑150 (MQ=255)                                                                                                                             cATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGc            <  3:103999/150‑1 (MQ=255)                                                                                                                                 gCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTCTGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGATGCTACt        >  3:500214/1‑150 (MQ=255)                                                                                                                                       aTCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGc  >  2:265534/1‑150 (MQ=255)                                                                                                                                            |                                                                                                                                                 AAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGC  >  NC_000913_3_pae_tpiA/1756554‑1756837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑