Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 349,624 C→T intergenic (+52/‑388) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA349,6240CT90.0% 265.6 / 16.2 110intergenic (+52/‑388)prpB/prpC2‑methylisocitrate lyase/2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base C (7/4);  new base T (46/53);  total (53/57)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

CCATGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGAC  >  NC_000913_3_pae_tpiA/349478‑349764
                                                                                                                                                  |                                                                                                                                            
ccATGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGAc                                                                                                                                           <  3:733435/150‑1 (MQ=255)
 cATGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACt                                                                                                                                          >  1:1141280/1‑150 (MQ=255)
  aTGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACtt                                                                                                                                         <  2:271370/150‑1 (MQ=255)
     cAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATc                                                                                                                                      <  3:7913/150‑1 (MQ=255)
     cAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATc                                                                                                                                      >  3:984947/1‑150 (MQ=255)
      aGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCa                                                                                                                                     <  1:1101596/150‑1 (MQ=255)
      aGACCCGCAACGACCTGTACGAAAGCATCACCTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGCAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCa                                                                                                                                     <  3:872307/150‑1 (MQ=39)
            gCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTa                                                                                                                               >  3:576541/1‑150 (MQ=255)
                cGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg                                                                                                                           <  2:1451071/150‑3 (MQ=255)
                cGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg                                                                                                                           <  1:1486788/150‑3 (MQ=255)
                  ggCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGt                                                                                                                         <  1:1238813/149‑1 (MQ=255)
                  aGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGt                                                                                                                         <  2:355631/150‑1 (MQ=255)
                  aGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGt                                                                                                                         <  2:101119/150‑1 (MQ=255)
                   gCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTg                                                                                                                        >  4:770928/1‑150 (MQ=255)
                     tGTACGAAAGCAGCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCa                                                                                                                      <  4:604223/150‑1 (MQ=38)
                      gTACGAAAGCATCAACTACTACCAGTACGAAGACAAGCTCGACAACCTGTCTGCACGTGGGTTCATACATTTACACTCGCCCGTTGTTTTTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCAc                                                                                                                     <  1:1396819/150‑1 (MQ=12)
                       tACGAAAGCATCAACTACTCCTATTACGAAGAGGAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTCTTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACt                                                                                                                    <  2:780534/150‑1 (MQ=37)
                         cGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGa                                                                                                                  >  2:848722/1‑150 (MQ=255)
                          gAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGaa                                                                                                                 >  3:811199/1‑150 (MQ=255)
                          gAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGaa                                                                                                                 >  3:321541/1‑150 (MQ=255)
                          gAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGaa                                                                                                                 >  2:780939/1‑150 (MQ=255)
                          gAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGaa                                                                                                                 <  3:148038/150‑1 (MQ=255)
                          gAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGaa                                                                                                                 <  1:560596/150‑1 (MQ=255)
                            aaGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACt                                                                                                               >  2:1176694/1‑150 (MQ=255)
                              gCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGt                                                                                                             <  1:780939/150‑1 (MQ=255)
                               cATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTa                                                                                                            <  1:1257059/150‑1 (MQ=255)
                                  cAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGGCTACGAGGTGCACTGAACTGGAggg                                                                                                         >  2:221867/1‑149 (MQ=255)
                                   aaCTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTc                                                                                                        <  1:525419/150‑1 (MQ=255)
                                      tactacCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAgg                                                                                                                                   <  1:809146/120‑1 (MQ=255)
                                      tactacCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAgg                                                                                                                                   >  2:809146/1‑120 (MQ=255)
                                        ctacCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATa                                                                                                   >  2:171069/1‑150 (MQ=255)
                                          acCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGAATTATCAGGCCTACGGGGTGCACTGCACTGTAGGTCGGATAAg                                                                                                 >  1:1118277/1‑150 (MQ=25)
                                           ccAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa                                                                                                >  1:44464/1‑150 (MQ=255)
                                           ccAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa                                                                                                <  1:200880/150‑1 (MQ=255)
                                            cAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGa                                                                                                                                            <  1:794327/105‑1 (MQ=255)
                                            cAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGa                                                                                                                                            >  2:794327/1‑105 (MQ=255)
                                            cAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa                                                                                                     <  1:1206262/144‑1 (MQ=255)
                                             cgTACGGAGAGAAGCTGGACGACCTGATTCCGGGCAGTGAGGAGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATATGACg                                                                                              <  2:135021/149‑1 (MQ=17)
                                              gTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACgc                                                                                             <  2:44464/150‑2 (MQ=255)
                                                 cGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATaa                                                                                                  <  1:1217239/142‑1 (MQ=255)
                                                 cGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATaa                                                                                                  >  2:1217239/1‑142 (MQ=255)
                                                 cGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACgcata                                                                                          >  3:24945/1‑146 (MQ=21)
                                                  gAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCAtag                                                                                         >  1:529979/1‑148 (MQ=21)
                                                   aaGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa                                                                                                     >  3:859147/1‑137 (MQ=255)
                                                   aaGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa                                                                                                     <  4:859147/137‑1 (MQ=255)
                                                   aaGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa                                                                                                     >  1:166555/1‑137 (MQ=255)
                                                    agagAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCg                                                                                       >  4:739457/1‑150 (MQ=21)
                                                     gagaAGCTCGACAACCTGTTGGCCCGTAGCCAGGTGAAAGATAAAATGCACGTTGATGGGATTCGACAGCCGATGCCCGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGGGCAAGTGACTGTAAGTATGAAAAGACGCATAGcta                                                                                      >  4:306732/1‑148 (MQ=12)
                                                      agaAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc                                                                                     <  2:921154/150‑1 (MQ=21)
                                                        aaGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGc                                                                                   >  2:201870/1‑150 (MQ=21)
                                                          gCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCt                                                                                                                                <  3:669143/103‑1 (MQ=255)
                                                          gCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCt                                                                                                                                >  4:669143/1‑103 (MQ=255)
                                                          gCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCAt                                                                                 >  2:149723/1‑150 (MQ=18)
                                                          gCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCAt                                                                                 <  3:305745/150‑1 (MQ=18)
                                                          gCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCAt                                                                                 <  1:644646/150‑1 (MQ=18)
                                                           cTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATc                                                                                >  2:1344216/1‑150 (MQ=18)
                                                            tCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTATGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATcc                                                                               >  2:1123371/1‑150 (MQ=255)
                                                             cGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCg                                                                              <  4:576541/150‑1 (MQ=18)
                                                              gACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa                                                                             <  1:1176694/150‑1 (MQ=18)
                                                               aCAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGAc                                                                            <  1:1344216/150‑1 (MQ=18)
                                                                 aaCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTc                                                                                                        >  1:1588813/1‑120 (MQ=21)
                                                                 aaCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTc                                                                                                        <  2:1588813/120‑1 (MQ=21)
                                                                 aaCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACaa                                                                          <  1:415093/150‑1 (MQ=18)
                                                                  aCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAc                                                                         <  4:121239/150‑1 (MQ=18)
                                                                   ccTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACgg                                                                                                                            <  2:1478988/98‑2 (MQ=255)
                                                                   ccTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACgg                                                                                                                            >  1:1478988/1‑97 (MQ=255)
                                                                   ccTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAcc                                                                        >  3:481639/1‑150 (MQ=17)
                                                                   ccTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAcc                                                                        >  3:403992/1‑150 (MQ=17)
                                                                   ccTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACAGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAcc                                                                        <  2:86561/150‑1 (MQ=18)
                                                                   ccTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAcc                                                                        >  3:414958/1‑150 (MQ=17)
                                                                     tGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGa                                                                      <  3:886051/150‑1 (MQ=17)
                                                                       tttGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTTGGTCGGATAAGACGCATAGCGTCGCATACGACAACCGATg                                                                    >  1:228868/1‑150 (MQ=17)
                                                                        ttGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGc                                                                   >  1:941913/1‑150 (MQ=17)
                                                                        ttGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGc                                                                   <  1:201870/150‑1 (MQ=17)
                                                                        tggCCCGTAACCAGGAGGAAGAAAAAACGCCCGTTGATTGTATTCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGc                                                                   <  2:941913/148‑1 (MQ=11)
                                                                            ccGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACg                                                                                                                             <  2:1111946/88‑1 (MQ=255)
                                                                            ccGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACg                                                                                                                             <  2:1111882/88‑1 (MQ=255)
                                                                            ccGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACg                                                                                                                             >  1:1111946/1‑88 (MQ=255)
                                                                            ccGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACg                                                                                                                             >  1:1111882/1‑88 (MQ=255)
                                                                             ggTAGCCAGGTGAAAGAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGAt                                                              <  2:1368825/149‑1 (MQ=14)
                                                                              gTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATg                                                             <  1:203256/150‑1 (MQ=17)
                                                                                  ccAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc                                                                                     >  4:380349/1‑122 (MQ=14)
                                                                                  ccAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc                                                                                     <  3:380349/122‑1 (MQ=17)
                                                                                  ccAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgcc                                                         >  3:215488/1‑150 (MQ=14)
                                                                                  ccAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgcc                                                         >  1:893701/1‑150 (MQ=14)
                                                                                   cAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGc                                                                                                                                  <  1:812848/76‑1 (MQ=25)
                                                                                   cAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGc                                                                                                                                  >  2:812848/1‑76 (MQ=25)
                                                                                     ggTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCt                                                      <  4:565310/150‑1 (MQ=255)
                                                                                     ggTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTa                                                                                                                               >  1:96042/1‑77 (MQ=21)
                                                                                     ggTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTa                                                                                                                               <  2:96042/77‑1 (MQ=21)
                                                                                     ggTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGt                                                                                                         <  4:854292/99‑1 (MQ=18)
                                                                                     ggTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGt                                                                                                         >  3:854292/1‑99 (MQ=18)
                                                                                      gTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTg                                                     >  2:146750/1‑150 (MQ=14)
                                                                                          aaTAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACgc                                                 <  4:215488/150‑2 (MQ=11)
                                                                                                    gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGc                                                                   >  4:447383/1‑122 (MQ=11)
                                                                                                    gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGc                                                                   <  3:447383/122‑1 (MQ=1)
                                                                                                           gATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCa                                                                                                                      <  2:914242/64‑1 (MQ=11)
                                                                                                           gATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCa                                                                                                                      >  1:914242/1‑64 (MQ=11)
                                                                                                                  ttCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCa                         >  1:737252/1‑150 (MQ=255)
                                                                                                                                         tCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGCATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGAc  >  2:1409898/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
CCATGCAGACCCGCAACGAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGAC  >  NC_000913_3_pae_tpiA/349478‑349764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: