New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 4040686	90 (1.550)	11 (0.200) +ATGT	9/270	6.1	14.8%	intergenic (+50/+220)	rrfA/mobB	5S ribosomal RNA of rrnA operon/molybdopterin‑guanine dinucleotide biosynthesis protein B
	?	NC_000913_3_pae_tpiA	= 4213071	40 (0.690)					noncoding (116/120 nt)	rrfE	5S ribosomal RNA of rrnE operon
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/4040605‑4040686 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCC  <  NC_000913_3_pae_tpiA/4213071‑4212961                                                                                   ||||                                                                                                                TCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGAGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACT                                                 >  1:392788/1‑150                         CCAGACATCAAATAAAACAAAAGGCTCAGTCGGAAGACTGGGCCTTTTGTTTTATCTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGT                         >  3:335160/1‑150                                      AAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGAGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGC            >  2:208926/1‑150                                       AAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGAGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCT           >  1:602349/1‑150                                          CGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATAGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACT        >  3:77419/1‑150                                             AAGGCTCAGTCGGAAGACTGGGCCTTTTGTTTTATCTGATGTCTGGCAGTTCCCTACTCTCGC                                                                                            <  3:291676/63‑1                                             AAGGCTCAGTCGGAAGACTGGGCCTTTTGTTTTATCTGATGTCTGGCAGTTCCCTACTCTCGC                                                                                            >  4:291676/1‑63                                             AAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCC     >  1:173327/1‑150                                              AGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGAGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCG    >  4:312832/1‑150                                              AGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGAGGAGACCCCACACTACCATCGGCGATACTGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCG    >  3:380515/1‑150                                                GCTCAGTCGGAAGACTGGGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGAGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGTCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCC  >  2:94575/1‑150                                                                                   ||||                                                                                                                TCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/4040605‑4040686 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCC  <  NC_000913_3_pae_tpiA/4213071‑4212961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.