Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,110,747 G→T intergenic (+62/+784) cdh → / ← yiiQ CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,110,7470GT95.4% 301.2 / 3.0 87intergenic (+62/+784)cdh/yiiQCDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (41/42);  total (42/45)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCA  >  NC_000913_3_pae_tpiA/4110600‑4110890
                                                                                                                                                   |                                                                                                                                               
tGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAggctgc                                                                                                                                               <  3:93171/150‑1 (MQ=255)
    ggCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCccg                                                                                                                                           >  2:187407/1‑150 (MQ=255)
         cacaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCcgcg                                                                                                                                      <  1:436200/150‑1 (MQ=255)
          acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGa                                                                                                                                     >  1:139148/1‑150 (MQ=255)
           caACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGTGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAg                                                                                                                                    <  3:410837/150‑1 (MQ=255)
            aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGc                                                                                                                                   <  2:191883/150‑1 (MQ=255)
            aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGc                                                                                                                                   >  2:77835/1‑150 (MQ=255)
             aCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATNCAGGAACATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGGTAAGGAAAGTAAGTGCCGGATATGAAAACCGTCAAATGTCAGACTTAACGCTGCCCCGCGGNGAGCg                                                                                                                                  >  1:253001/1‑150 (MQ=255)
              cGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCgg                                                                                                                                 >  2:606918/1‑150 (MQ=255)
               gAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGt                                                                                                                                >  1:315910/1‑150 (MQ=255)
                  aTCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTc                                                                                                                             <  2:426281/150‑1 (MQ=255)
                     tACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGcc                                                                                                                          <  2:511597/150‑1 (MQ=255)
                        tGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAAt                                                                                                                       >  3:226739/1‑150 (MQ=255)
                            gCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTa                                                                                                                   >  2:205002/1‑150 (MQ=255)
                             cTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTaa                                                                                                                  >  1:556478/1‑150 (MQ=255)
                                    gTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTa                                                                                                           <  2:570084/150‑1 (MQ=255)
                                          cAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGatat                                                                                                     <  1:606918/150‑1 (MQ=255)
                                             ccGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATggg                                                                                                  >  2:340978/1‑150 (MQ=255)
                                              cGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGt                                                                                                 >  2:96527/1‑150 (MQ=255)
                                              cGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGt                                                                                                 <  2:315910/150‑1 (MQ=255)
                                               gAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTc                                                                                                >  4:256621/1‑150 (MQ=255)
                                               gAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTc                                                                                                <  1:96527/150‑1 (MQ=255)
                                                aGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCt                                                                                               >  4:115295/1‑150 (MQ=255)
                                                aGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCt                                                                                               >  1:327788/1‑150 (MQ=255)
                                                 ggAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCtt                                                                                              >  3:301936/1‑150 (MQ=255)
                                                  gAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCttt                                                                                             >  1:426114/1‑150 (MQ=255)
                                                  gAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCttt                                                                                             >  3:174749/1‑150 (MQ=255)
                                                  gAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCttt                                                                                             >  3:174788/1‑150 (MQ=255)
                                                       tCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGTCACCTGGCAGACTTAATGCTGCCCCGCCGCGAGCGGTTTCGGCAATTCTAAGATCTTATGATATGGGTCTTTTGCCt                                                                                        >  1:671370/1‑150 (MQ=255)
                                                         aGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTc                                                                                      >  1:157020/1‑150 (MQ=255)
                                                         aGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTc                                                                                      <  4:124865/150‑1 (MQ=255)
                                                          ggATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCa                                                                                     <  1:516295/150‑1 (MQ=255)
                                                          ggATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCa                                                                                     <  1:27088/150‑1 (MQ=255)
                                                           gATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCAc                                                                                    <  1:614470/150‑1 (MQ=255)
                                                            atcatcAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACg                                                                                   >  4:362985/1‑150 (MQ=255)
                                                             tcatcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACgg                                                                                  <  2:626898/150‑1 (MQ=255)
                                                                tcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGACGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTg                                                                               <  2:28213/150‑1 (MQ=255)
                                                                  aGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgcg                                                                             >  3:365544/1‑150 (MQ=255)
                                                                   gtgtGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgcgc                                                                            <  3:325201/150‑1 (MQ=255)
                                                                      tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACgg                                                                                  >  1:638567/1‑141 (MQ=255)
                                                                      tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACgg                                                                                  <  2:638567/141‑1 (MQ=255)
                                                                      tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTg                                                                         >  1:305838/1‑150 (MQ=255)
                                                                       gagaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGc                                                                        <  4:107484/150‑1 (MQ=255)
                                                                        agaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTGCGCCGATTCTAAGATCTTAAGAGATGGGTCGTTTGCCTTCACGGCTGCGCTTGcc                                                                       >  3:232896/1‑150 (MQ=255)
                                                                         gaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCa                                                                      <  1:187407/150‑1 (MQ=255)
                                                                         gaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCa                                                                      <  1:491303/150‑1 (MQ=255)
                                                                           ttttGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGc                                                                    >  2:420907/1‑150 (MQ=255)
                                                                                   tAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTcc                                                            <  2:139148/150‑1 (MQ=255)
                                                                                    agggCGAAGAGTTAAGGACAGTAAGTGCCGGACATGAAATCCGGGACGTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTccc                                                           <  4:328772/148‑1 (MQ=255)
                                                                                              gTTAAGGAAAGTAAGCGCCGGATAAGAAATCCGGCACCTGTCAGACATACGGCGGCCCCGCCGCGAGCGGTTGCGCGAATTGTAAGTGCTTAAGATATGGGTCGGTGGCCATCAGGGCGGCGCCTGCCAGCAACGCTCCCCACGTGCCGa                                                 <  2:354569/150‑1 (MQ=255)
                                                                                                 aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCg                                                                                                                            <  2:251648/72‑1 (MQ=255)
                                                                                                 aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCg                                                                                                                            >  1:251648/1‑72 (MQ=255)
                                                                                                 aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCaa                                                                                                                        >  2:353066/1‑76 (MQ=255)
                                                                                                 aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCaa                                                                                                                        <  1:353066/76‑1 (MQ=255)
                                                                                                   ggAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCGCGCCGCGAGCGTTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCGTTCCAGCAACACTCCCCTCGTGCCGAGTTTa                                            >  2:401729/1‑150 (MQ=255)
                                                                                                   ggAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTa                                            <  1:420907/150‑1 (MQ=255)
                                                                                                    gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTaa                                           <  2:365200/150‑1 (MQ=255)
                                                                                                    gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTaa                                           >  2:18323/1‑150 (MQ=255)
                                                                                                    gAAAGTAAGTGCCGGATATGAAATCCGGCACATGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTaa                                           <  2:253001/150‑1 (MQ=255)
                                                                                                         taagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGCTCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGCGTTTAAGcgcg                                      <  2:671370/150‑1 (MQ=255)
                                                                                                         taagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGcgcg                                      <  3:115295/150‑1 (MQ=255)
                                                                                                          aagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCgg                                     >  4:184656/1‑150 (MQ=255)
                                                                                                            gtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCt                                   <  4:174749/150‑1 (MQ=255)
                                                                                                            gtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCt                                   <  4:174788/150‑1 (MQ=255)
                                                                                                             tGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCtt                                  >  4:185591/1‑150 (MQ=255)
                                                                                                              gCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTc                                 <  1:205002/150‑1 (MQ=255)
                                                                                                                cGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCaa                               >  1:235296/1‑150 (MQ=255)
                                                                                                                 ggATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                              <  2:426114/150‑1 (MQ=255)
                                                                                                                 ggATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                              <  4:53724/150‑1 (MQ=255)
                                                                                                                     atGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTcc                          >  3:242176/1‑150 (MQ=255)
                                                                                                                        aaaTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGcc                                                                       <  1:9899/102‑1 (MQ=255)
                                                                                                                        aaaTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGcc                                                                       >  2:9899/1‑102 (MQ=255)
                                                                                                                        aaaTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctct                       >  4:336893/1‑150 (MQ=255)
                                                                                                                          aTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTcc                     <  3:336893/150‑1 (MQ=255)
                                                                                                                          aTCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTcc                     <  1:18323/150‑1 (MQ=255)
                                                                                                                             cGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGct                  <  3:206780/150‑1 (MQ=255)
                                                                                                                               gCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctct                <  3:185591/150‑1 (MQ=255)
                                                                                                                                cACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCtctc               <  3:9251/150‑1 (MQ=255)
                                                                                                                                   cTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAAt            <  1:168950/150‑1 (MQ=255)
                                                                                                                                     gTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAc          >  2:552507/1‑150 (MQ=255)
                                                                                                                                     gTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAc          >  2:101744/1‑150 (MQ=255)
                                                                                                                                     gTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAc          >  4:209731/1‑150 (MQ=255)
                                                                                                                                      gcAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAcc         <  2:640238/149‑1 (MQ=255)
                                                                                                                                        aGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGc                                                                        <  1:233039/85‑1 (MQ=255)
                                                                                                                                        aGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGc                                                                        >  2:233039/1‑85 (MQ=255)
                                                                                                                                        aGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCg       >  1:454645/1‑150 (MQ=255)
                                                                                                                                             tAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCa  <  3:184656/150‑1 (MQ=255)
                                                                                                                                             tAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCa  >  3:61460/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCA  >  NC_000913_3_pae_tpiA/4110600‑4110890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑