Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	264,098	0	G	T	98.6%	214.5 / NA	71	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (37/33);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAATTTTCA  >  NC_000913_3_pae_tpiA/263952‑264246                                                                                                                                                   |                                                                                                                                                     cGGGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAg                                                                                                                                                    <  2:786535/149‑1 (MQ=255)      tCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTc                                                                                                                                               >  1:311688/1‑149 (MQ=255)          aGGCTCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCACGTATAGGGCCCATCGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGc                                                                                                                                           <  1:294035/149‑1 (MQ=255)              gcAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGata                                                                                                                                       >  1:121743/1‑149 (MQ=255)                      aTCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGATCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAAc                                                                                                                               <  2:35302/149‑1 (MQ=255)                         tcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGGTATCCGGAACGTc                                                                                                                            >  2:90385/1‑149 (MQ=255)                         tcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTc                                                                                                                            <  1:62646/149‑1 (MQ=255)                         tcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACGATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTCTACCGCTAGGAATCCGGCGATCTCGGGAACCTc                                                                                                                            >  2:28830/1‑149 (MQ=255)                          cccGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTGAGTTCAGGTGGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTcc                                                                                                                           <  2:31127/149‑1 (MQ=255)                          cccGCTCCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTcc                                                                                                                           <  1:374817/149‑1 (MQ=255)                             gCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTGGGGCTCCGGCGATATCCGGAACGTCCCTg                                                                                                                        >  1:327645/1‑149 (MQ=255)                              cTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGc                                                                                                                       <  1:512818/149‑1 (MQ=255)                              cTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACGATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGc                                                                                                                       <  2:614908/149‑1 (MQ=255)                                 cAGGCCCCTGGTAGGGTTGCCCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCCCTAGGACTCCGGCGATATCCGGGACGTCCCTGCCgg                                                                                                                    >  2:876605/1‑149 (MQ=255)                                  aGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                                                                                                                   <  1:98149/149‑1 (MQ=255)                                   ggCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGCATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                                                                                                                  >  1:27453/1‑149 (MQ=255)                                      cccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTaa                                                                                                               >  2:576743/1‑149 (MQ=255)                                          ggTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGAc                                                                                                           <  2:121743/149‑1 (MQ=255)                                                 ttGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGa                                                                                                                                         <  2:850305/112‑1 (MQ=255)                                                 ttGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGa                                                                                                                                         >  1:850305/1‑112 (MQ=255)                                                    cTCATTACAGTTCTCCACTATTGTCAGTACAGGGGGATGCTCATCAGGCAGATATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGTTAGGACTCCGGCGATAGCCGGAACGTCCCTGCCGGGGTGAGGACGGGCGCTGcc                                                                                                 >  2:841749/1‑149 (MQ=255)                                                     tCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCGACCg                                                                                                >  2:766828/1‑149 (MQ=255)                                                             gTTCTCCACTATTGTCAGGTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTg                                                                                        <  2:113063/149‑1 (MQ=255)                                                                ctcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTa                                                                                     <  2:374124/149‑1 (MQ=255)                                                                 tcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAt                                                                                    >  2:845340/1‑149 (MQ=255)                                                                 tcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAt                                                                                    >  1:136176/1‑149 (MQ=255)                                                                   cACTATTGTCAGGTCAGGGTTATGCTCATCAGACAGGTATAACGCCCATGGCGATCCTGTCGCCGCTCGGCGTATACCGCTAGCACTCCGCCGATATCCGTAACGTCCCGGCCCGTGCAATGACAGACGCTACCGTGCCACCGGTCttt                                                                                  >  2:697575/1‑149 (MQ=18)                                                                    aCTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTg                                                                                 >  2:947600/1‑149 (MQ=255)                                                                    aCTATTGTCAGTTCAGGGTGATGCTAATCCGCCGGGTAGAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTg                                                                                 >  1:267582/1‑149 (MQ=21)                                                                       gttGTCAGTGCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCTTGGCGGCGTTCGGCGCATCCCGCTAGGAGTCCGGCGGTATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCg                                                                              <  2:833/148‑1 (MQ=21)                                                                       aTTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCg                                                                              <  1:866184/149‑1 (MQ=255)                                                                           tCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                                                                                                                  <  2:388274/109‑1 (MQ=255)                                                                           tCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                                                                                                                  >  1:388274/1‑109 (MQ=255)                                                                           tCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGc                                                                          >  1:314151/1‑149 (MQ=255)                                                                           tCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGc                                                                          >  1:478479/1‑149 (MQ=255)                                                                              gTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGt                                                                       >  1:655834/1‑149 (MQ=255)                                                                               ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGCGTAATGACAGACGCCACCGTGCGACTGGTATTTGCCGGTGCACTa                                                                      >  2:885971/1‑149 (MQ=255)                                                                                   gggTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGa                                                                  >  2:245837/1‑149 (MQ=255)                                                                                    ggTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGTTGCAGTAGCGaa                                                                 >  1:144214/1‑149 (MQ=255)                                                                                    ggTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGaa                                                                 <  2:478479/149‑1 (MQ=255)                                                                                     gTGATGGTCATCAGGCAGGTATAGGGCCCATTGCGGGCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATAGCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGaaa                                                                <  1:368755/149‑1 (MQ=255)                                                                                      tGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAg                                                               >  1:895843/1‑149 (MQ=255)                                                                                       gATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGa                                                              <  1:947600/149‑1 (MQ=255)                                                                                          gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTg                                                                                        <  1:526591/120‑1 (MQ=255)                                                                                          gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTg                                                                                        >  2:526591/1‑120 (MQ=255)                                                                                            tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGAtt                                                            >  1:341457/1‑146 (MQ=255)                                                                                            tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGAtt                                                            <  2:341457/146‑1 (MQ=255)                                                                                            atcctaggcaggTATGGGGCCCATGGCGGTCCTGGCGGCGTTCGGCGTATCCCGCTAGGACTCCGGCGATATCCGGAACGTCCTTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCgg                                                         <  1:1055352/144‑1 (MQ=18)                                                                                              atcaGGCAGGTATAGGGCCCATTGGGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGTCTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGa                                                       <  1:772411/149‑1 (MQ=255)                                                                                              atcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGa                                                       >  1:1041843/1‑149 (MQ=255)                                                                                                  ggtaggTATAGGGCGCATGGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCa                                                   <  2:707267/146‑1 (MQ=255)                                                                                                   gcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGGTATCCGGAACGTCCCTGCCGGTGTAATGACAGAAGCTACAGTGCCACTGGTATTTGGCGGTGCCGTACCGAAAGATTCGGGCCtacg                                                  >  2:433249/1‑146 (MQ=18)                                                                                                   gcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGCACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAg                                                  <  1:368328/149‑1 (MQ=255)                                                                                                     aggTGTAGGGCCCATTGGGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGa                                                <  2:994250/149‑1 (MQ=255)                                                                                                            gggCCCATTGCGGTCCTGGCGGCGTTCGGCTTATACCGCTAGGACTCCGGCGACCTCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGc                                         <  1:90385/149‑1 (MQ=255)                                                                                                            gggCCCATTGCGGTCCTGGCGGCGGTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGc                                         <  1:411317/149‑1 (MQ=255)                                                                                                             ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCg                                        >  1:631214/1‑149 (MQ=255)                                                                                                               cccATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGa                                                                                                                                                  >  1:337435/1‑41 (MQ=255)                                                                                                               cccATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGa                                                                                                                                                  <  2:337435/41‑1 (MQ=255)                                                                                                                   ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGACTCGGGCCTCAGGATGCTGGCGGTatat                                  >  1:1027989/1‑149 (MQ=255)                                                                                                                    tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCAGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATc                                 <  1:499430/149‑1 (MQ=255)                                                                                                                      cGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGCCAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGt                               >  1:648869/1‑149 (MQ=255)                                                                                                                       ggTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCCACCAATTCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCAGTATATCGTc                              >  2:514129/1‑149 (MQ=17)                                                                                                                             ggcggcGTTCGGGGTATACCGCTAGGCCTCCGGCGATATCCGGAACGTCCCTGGCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGTTGGCGGTATATCGTCATTGcc                        <  2:973885/149‑1 (MQ=17)                                                                                                                              gcggcgTTCGGCGTATACTGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTGCCGAAAAATTCGGGACTGAGGATGCGGGCGGTATTTCGTCATTGccc                       >  1:411403/1‑149 (MQ=14)                                                                                                                              gcggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGccc                       >  2:716403/1‑149 (MQ=255)                                                                                                                                    ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATAGTCTTGGTCCTGCGtt                 >  2:726166/1‑149 (MQ=17)                                                                                                                                      cGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTa               <  2:827809/149‑1 (MQ=255)                                                                                                                                       ggCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGccc                       >  1:701937/1‑140 (MQ=255)                                                                                                                                       ggCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGccc                       <  2:701937/140‑1 (MQ=255)                                                                                                                                             taCCGCGAGGGCTCCGGCGATATCCGGAGCGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCACTAGCCAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATaa        <  1:114064/149‑1 (MQ=255)                                                                                                                                                   gAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGGGCCACTGGTATTTGCCGGTGGAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAACTTTCa  <  2:1071960/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CGGGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAATTTTCA  >  NC_000913_3_pae_tpiA/263952‑264246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑