New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 434171144 (1.130)4 (0.120) 4/250 4.8 9.4% intergenic (‑167/‑116) melR/melA melibiose operon transcriptional regulator; autoregulator/alpha‑galactosidase, NAD(P)‑binding
?NC_000913_3_pae_tpiA = 4341731 39 (1.150)intergenic (‑187/‑96) melR/melA melibiose operon transcriptional regulator; autoregulator/alpha‑galactosidase, NAD(P)‑binding
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/4341552‑4341711
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑attcccataaactcagatCCTGCGTGAAGCAGCAGTAAATCTGAGTTTATGGGAATAAATTACCTGAAAAGCAGAGTTTTCGGCCTCATGATACTCGGAGAGTCCCCTCGCCCCTCTGGGAAGAGGGTGAAAACCGTGCTCCCACTCGCAGTCATCCTCCC  <  NC_000913_3_pae_tpiA/4341731‑4341589
                                                                                                                                                                                                                                                                                                               
TCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCga                                                                                                                                                            >  2:111682/1‑147
TCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCA                                                                                                                                                            >  1:482915/1‑149
       CCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGAGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTC                                                                                                                                                     >  1:546157/1‑149
          GGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTTCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGA                                                                                                                                                  <  2:93236/149‑1
           GAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCCGAT                                                                                                                                                 >  1:295092/1‑149
                                                            CACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATCCTGCGTGAAGCAGCAGTAAATCTGAGTTTAT                                                                                                                 <  2:502594/132‑1
                                                            CACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATCCTGCGTGAAGCAGCAGTAAATCTGAGTTTAT                                                                                                                 >  1:502594/1‑132
                                                                                CAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCC                                                                                                                                                             >  1:259514/1‑68
                                                                                CAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCC                                                                                                                                                             <  2:259514/68‑1
                                                                                 AGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATCCTGCGTGAAGCAGCAGTAAATCTGAGTTTATGGGAATAAATTACCTGAAA                                                                                              <  2:576842/130‑1
                                                                                 AGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATCCTGCGTGAAGCAGCAGTAAATCTGAGTTTATGGGAATAAATTACCCGAAA                                                                                              >  1:576842/1‑130
                                                                                                                                                TCCCATAAACTCAGATCCTGCGTCAAGCAGCAGTAAATCTGAGTTTATGGGAATAAATTACCTGAAAAGCAGAGTTTTCGGCCTCATGATACTCGGAGAGTCCCCTCGCCCCTCTGGGAAGAGGGTGAAAACCGTGCTCCCACTCGCAG            <  1:231405/149‑1
                                                                                                                                                  CCATAAACTCAGATCCTGCGTGAAGCAGCAGTAAATCTGAGTTTATGGGAATAAATTACCTGAAAAGCAGAGTTTTCGGCCTCATGATACTCGGAGAGTCCCCTCGCCCCTCTGGGAAGAGGGTGAAAACCTTGCTCCCACTCGCAGTC          >  1:128600/1‑149
                                                                                                                                                   CATAAACTCAGATCCTGCGTGAAGCAGCAGTAAATCTGAGTTTATGGGAATAAATTACCTGAAAAGCAGAGTTTTCGGCCTCATGATACTCGGAAAGTCCCCTCGCCCCTCTGGGAAGAGGGTGAAAACCGTGCTCCCACTCGCAGTCA         >  1:265476/1‑149
                                                                                                                                                       AACTCAGATCCTGCGTGAAGCAGCAGTAAATATGAGTTTATGGGAATAAATTACCTGAAAAGCAGAGTTTTCGGCCTCATGATACTCGGAGAGTCCGCTCGCCCCTCTGGGAAGAGG                                     <  2:388712/117‑1
                                                                                                                                                       AACTCAGATCCTGCGTGAAGCAGCAGTAAATATGAGTTTATGGGAATAAATTACCTGAAAAGCAGAGTTTTCGGCCTCATGATACTCGGAGAGTCCCCTCGCCCCTCTGGGAAGAGG                                     >  1:388712/1‑117
                                                                                                                                                        ACTCAGATCCTGCGTGAAGCAGCAGTAAATCTGAGTTTATGGGAATAAATTACCTGAAAAGCAGAGTTTTCGGCCTCATGATACTCGGAGAGTCCCCTCGCCCCTCTGGGAAGAGGGTGAAAACCGTGCTCCCACTCGCAGTCATCCT     >  1:58288/1‑148
                                                                                                                                                        ACTCAGATCCTGCGTGAAGCAGCAGTAAATCTGAGTTTATGGGAATAAATTACCTGAAAAGCAGAGTTTTCGGCCTCATGATACTCGGAGAGTCCCCTCGCCCCTCTGGGAAGAGGGTGAAAACCGTGCTCCCACTCGCAGTCATCCT     <  2:58288/148‑1
                                                                                                                                                          TCAGATCCTGCGTGAAGCAGCAGTAAATCTGAGTTTATGGGAATAAATTACCTGAAAAGCAGAGTTTGCGGCCTCATGATACTCGGAGAGTCCCCTCGCCCCTCTGGGAAGAGGGTGAAAACCGTGCTCCCACTCGCAGTCATCCTCCC  <  1:670238/149‑1
                                                                                                                                                                                                                                                                                                               
TCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/4341552‑4341711
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑attcccataaactcagatCCTGCGTGAAGCAGCAGTAAATCTGAGTTTATGGGAATAAATTACCTGAAAAGCAGAGTTTTCGGCCTCATGATACTCGGAGAGTCCCCTCGCCCCTCTGGGAAGAGGGTGAAAACCGTGCTCCCACTCGCAGTCATCCTCCC  <  NC_000913_3_pae_tpiA/4341731‑4341589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.