New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	3587430 =	40 (1.030)	4 (0.110)	4/266	5.1	8.9%	coding (684/744 nt)	ugpQ	glycerophosphodiester phosphodiesterase, cytosolic
	?	NC_000913_3_pae_tpiA	3587464 =	45 (1.240)					coding (650/744 nt)	ugpQ	glycerophosphodiester phosphodiesterase, cytosolic
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAA  >  NC_000913_3_pae_tpiA/3587328‑3587612                                                                                                                                         |                                                                                                                                                     tGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGtat                                                                                                                                          >  1:659750/1‑149 (MQ=255)                   cgcAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTc                                                                                                                        <  2:583062/149‑1 (MQ=255)                       gCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGc                                                                                                                    <  1:216199/149‑1 (MQ=255)                               ttCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCa                                                                                                            >  2:363899/1‑149 (MQ=255)                                 ccGTTGCAAACTATTGAGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCCCACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTACAAACCAGAATCCGCAGTCCTGCGTCTTTCAAc                                                                                                          >  2:221638/1‑149 (MQ=255)                                 ccGTTGAAAACGATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAAc                                                                                                          <  2:416001/149‑1 (MQ=255)                                     tGAAAACTATTGGGCCGTAAAGTTCGAACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCCGCGCAGCAACCCTGCTGCGCGCTGCGGTTTATTGACGGTATACACCAGACTCCGCAGTCCGGCGTCTTTCAACTGCa                                                                                                      <  2:164833/149‑1 (MQ=255)                                       aaaaCTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATc                                                                                                    <  2:103510/149‑1 (MQ=255)                                         aaCTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGTTGCAATCCACACGCCAGCGCAGCAACTCTGCTGCGCGCTGGGGGTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCGTTCAACTGCATCAc                                                                                                  >  1:366532/1‑149 (MQ=255)                                            tATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGGTGCAATCCACACCCGAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTcg                                                                                               >  1:601852/1‑149 (MQ=255)                                            tATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCAAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTcg                                                                                               >  1:601834/1‑149 (MQ=255)                                             aTTGCGCCTGAAAGTTCCTACCAACCACGTCAATCGCATCGGTACAGATGCAAGCCACACCCCAGCGCAGCAACTCTGTGGCGTGTTGTGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTcgc                                                                                              <  1:161277/149‑1 (MQ=255)                                                  gCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCttt                                                                                         >  1:438294/1‑149 (MQ=255)                                                     gTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTAtt                                                                                      >  2:364746/1‑149 (MQ=255)                                                     gTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTGTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTAtt                                                                                      <  1:363899/149‑1 (MQ=255)                                                      tAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTg                                                                                     >  1:43000/1‑149 (MQ=255)                                                       gaggTTCGGACCATTCACGTCAATCGCATGGGTACAGATGCAAGCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAATCCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGa                                                                                    <  2:340859/146‑1 (MQ=255)                                                       aaaGTTCGGACCAATTACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGa                                                                                    <  2:163442/149‑1 (MQ=255)                                                           ttCGGAGCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACCCGCGCTTTATTGGGTaa                                                                                >  1:677539/1‑149 (MQ=255)                                                                   aaTCACGTCAATCGCATCGGTACAGATGCACTCCCCCCGGCAGCTGAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCCCTTTATTGAGTAACTTTTGAt                                                                        >  1:336266/1‑149 (MQ=255)                                                                    aTCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGAtt                                                                       >  2:527433/1‑149 (MQ=255)                                                                    aTCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGAtt                                                                       <  1:562763/149‑1 (MQ=255)                                                                    aTCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGAtt                                                                       <  2:43000/149‑1 (MQ=255)                                                                      cACGACAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTTGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCTGGCGTCTTTCATCTGCATCATTCGCGCTTTATTGAGTAACTTATGATTga                                                                     <  1:577869/149‑1 (MQ=255)                                                                       aCGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAAAATCATCAGTGCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTgag                                                                    >  1:331219/1‑149 (MQ=255)                                                                          tCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATg                                                                 >  1:589723/1‑149 (MQ=255)                                                                                 cATCTGTACAGATGCAATCCACTCCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCCCAGTCCGGCGTCTTTCAACTGCATCCCTCGCTCTTTATTGAGTAACTTATGATTGAGATGAATagag                                                          <  2:554538/149‑1 (MQ=255)                                                                                 cATCGGTACATATCCAATCCACACCCCCGCCCAGCAACTCTCCTGCGCGCTGGGGCTCACTGACGGTATAAACCAGAATCCGCAGTCCGTCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATagag                                                          <  1:158396/149‑1 (MQ=255)                                                                                  aTCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAgaga                                                         >  1:358255/1‑149 (MQ=255)                                                                                     ggTACAGATACAATCAAAACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGc                                                      >  2:151273/1‑149 (MQ=255)                                                                                            aTGCAATCCACACCCCAGCGCAGCAACTCTGCTACGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTAATGATTGAGATGAATAGAGACGcagcccag                                               >  1:337631/1‑149 (MQ=255)                                                                                             tGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGcagcccagc                                              >  2:622032/1‑149 (MQ=255)                                                                                                   ccACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCACCTGCATCACTCGCGGTTTATTGAGTAACTTATGATTGAGATGAATAGAGAGGCAGCCCACCcgcgag                                        >  2:485611/1‑147 (MQ=255)                                                                                                   ccACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCCCGCTTTATTGAGTAACTTCTGATTGAGATGCATAGAGACGCAGCCCAGCcgcgcg                                        >  2:281996/1‑149 (MQ=255)                                                                                                       tccccAGCGCAGCAACTCTGCTGCCCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGACACGCAGCCCAGCCGCGCGGTCa                                    <  1:100224/148‑1 (MQ=255)                                                                                                          ccAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGCTGAATAGAGACGCAGCCCAGCCGCGCGGtgcgtt                                 >  2:543096/1‑144 (MQ=255)                                                                                                           cAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAGCTTATTATTGAGATGAATAGGCACGCAGCCCAGCCGCGCGGTCAGTTc                                >  1:433482/1‑149 (MQ=255)                                                                                                             gcgcAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTcgc                              <  1:151273/149‑1 (MQ=255)                                                                                                                 agcaACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTa                                                                                        <  2:407543/87‑1 (MQ=255)                                                                                                                 agcaACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTa                                                                                        >  1:407543/1‑87 (MQ=255)                                                                                                                 agcaACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTa                                                                            >  2:43176/1‑99 (MQ=255)                                                                                                                 agcaACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTa                                                                            <  1:43176/99‑1 (MQ=255)                                                                                                                   caACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAgt                        <  2:589723/149‑1 (MQ=255)                                                                                                                     aCTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAgtcg                      >  1:502094/1‑149 (MQ=255)                                                                                                                       tctGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAgtcgtc                    >  1:217702/1‑149 (MQ=255)                                                                                                                                 gcTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTGTTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTTGCGCCAGTCGTCGCGCCACTCa          >  1:435626/1‑149 (MQ=255)                                                                                                                                 gcTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCa          <  1:622032/149‑1 (MQ=255)                                                                                                                                       gTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCgcgc                >  1:615905/1‑137 (MQ=255)                                                                                                                                       gTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCgcgc                <  2:615905/137‑1 (MQ=255)                                                                                                                                       gTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTcagccagc    >  1:662020/1‑149 (MQ=255)                                                                                                                                       gTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGc    >  2:527454/1‑149 (MQ=255)                                                                                                                                         ttATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGcaa  <  1:527454/149‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                     TGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAA  >  NC_000913_3_pae_tpiA/3587328‑3587612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.