| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 4,295,976 | 0 | C | T | 29.2% | 56.6 / 26.2 | 49 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base C (17/17); new base T (9/5); total (27/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTA > NC_000913_3_pae_tpiA/4295831‑4296116 | gtaTAAGGCGTTTACGCCCCATCCGATATCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGcc < 1:370761/147‑1 (MQ=255) tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa > 1:460421/1‑149 (MQ=17) atgCGTTTCTGCCGGATCCGACATCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATg < 1:475485/147‑1 (MQ=255) ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGc < 1:599788/149‑1 (MQ=32) gCGGTTATGCCGCATCCGACATCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACCGCGCCATAGCCTGATTTAGCGTGATTTTCTAGGCCGGATAGGTCGTTTGCGCCACATCCGACCTCAAGGCCTGATGCg > 2:654015/1‑149 (MQ=11) ccGCATCCGACATCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc > 1:216206/1‑149 (MQ=25) gCATCCGACATCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTACGCCGCATCCAACATCAACGCCCGATGCGACCCTTGcctc > 2:252785/1‑147 (MQ=255) atcccgcATCAACGCCTGAAGCGAAGC‑TTCACTCGTATTAACAGACCTACGCCAGACAGCGCATATGCCTGATTCAGCATTAATTTGTAGTTCGGAGAAGGCGTTTACACCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt < 2:571971/143‑1 (MQ=11) tcggACATCAACGCCTGGTGCGACGC‑TTAAGGCGTCTTATCAGGCCTACGCTAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc < 1:355487/146‑1 (MQ=11) tCCGACATCAACGCCTGATGCGACGG‑TGATCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGTGACGCTTGCCGCGTc < 2:172084/149‑1 (MQ=11) cGACATCAACGCCTGACGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGGGATTTTGTAAGTCGAACAAGGCGTTTACGACCCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt < 1:270999/149‑1 (MQ=11) gaaaTCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGCTTCGACGCTTGCCTCGTTTTa > 2:71174/4‑149 (MQ=11) gACATCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCCACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTCCCCCGTCTTa > 2:500192/1‑149 (MQ=11) aTCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa > 2:542079/1‑149 (MQ=21) gtaggCCTGATAAGACGCGTTAA‑GCGTCGCATCAGGCGTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg > 1:413975/5‑149 (MQ=11) aaCGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg > 2:380253/1‑117 (MQ=32) aCGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGcc > 1:7923/1‑149 (MQ=25) gCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTAAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa > 2:596056/1‑149 (MQ=21) gATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATAAATGCCTGATGAGACGCGTGCCGGCTCTTATCAGGCCTAcctt > 1:494440/1‑146 (MQ=11) tGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCCTATCAGGCCTatcttac > 1:28375/1‑143 (MQ=9) cGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAggc > 1:639771/1‑147 (MQ=37) acggcgACTAATCAGCGCTACGTCACAAAGAGCCATAGCCTGATTTAGCGTAATTTTCTAGGTCGGATGAGGCGCTTACGCCGCATCCGACATCAACGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGAGCAAt < 2:227338/146‑1 (MQ=11) aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACGGCGCGATAGCCTGCttt > 1:250898/1‑149 (MQ=255) tgaGGCTTACGCGAGACAGCGCAATAGCCTAATTGAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATACGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa < 2:450171/147‑1 (MQ=11) gggctgaCGCCAGACAGCCCAATAGCCTGATTTGGCGTGCTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc < 2:331260/143‑1 (MQ=11) gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGt < 1:496828/149‑1 (MQ=39) gCCGACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGt < 1:51970/149‑1 (MQ=38) tACGCCAGACAGCGCAATAGCCTGGTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAACGCCATAGCTTTATTTAGCGTGAt > 2:279592/1‑149 (MQ=11) gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCGTACGCCAGACAGAGCAATAGCCAGCGGTAGCGTGAtgtt > 1:206248/1‑149 (MQ=11) gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt < 1:654391/149‑1 (MQ=39) gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGAGTTTACGCCGCATCAGACATCAACGCGTGATGCGACGCTTGTCGACTCTTATTAGACATACGCCAGCCAGGGGAACAGCGTGCTTTGACGTGAtgtt > 2:373703/1‑149 (MQ=11) gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAg > 2:646595/1‑149 (MQ=39) ccaTAGCATGATTTCGCGTGATTTTGTAGGTGTGATTAGTGGTTTACGCCGCATCCGACATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAt < 1:9955/147‑1 (MQ=11) acccTGATTTAGCGTGATTTTGTAGGTCGGACAAGGCGTTTACGCCCCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAgg < 1:106123/147‑1 (MQ=255) tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt < 2:63296/149‑1 (MQ=14) ttttGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt > 2:585566/1‑67 (MQ=25) ttttGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa > 2:177592/1‑107 (MQ=14) ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt < 1:585566/67‑1 (MQ=255) ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTTATGCCCCCTCCg > 2:310323/1‑149 (MQ=14) ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa < 1:177592/107‑1 (MQ=40) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc < 1:323895/149‑1 (MQ=38) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc < 1:323841/149‑1 (MQ=39) gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCt > 1:459155/1‑149 (MQ=255) aaGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAt < 1:521101/149‑1 (MQ=38) ggCGTTTAAGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa > 2:416143/1‑103 (MQ=255) ggCGTTTAAGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa < 1:416143/103‑1 (MQ=255) gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc > 1:129831/1‑98 (MQ=39) gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTACCTGGGACAAGCCGTTTATGCAGCAGAGGACAGCAACGCCTGATGCGACGCGTaa > 1:92071/1‑149 (MQ=11) gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGTGACGCTTACCGCGTCTTa > 2:467734/1‑149 (MQ=25) | GGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTA > NC_000913_3_pae_tpiA/4295831‑4296116 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |