Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,159,112	G→A	G236E (GGG→GAG)	oxyR →	oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,159,112	0	G	A	100.0%	290.9 / NA	83	G236E (GGG→GAG)	oxyR	oxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/46);  total (37/46)

AGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGAGCA  >  NC_000913_3_pae_tpiA/4158966‑4159256                                                                                                                                                   |                                                                                                                                                 aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGAt                                                                                                                                               <  1:1099424/150‑1 (MQ=255)    ggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCAc                                                                                                                                            >  1:545749/1‑150 (MQ=255)     gAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACt                                                                                                                                           >  1:1016101/1‑150 (MQ=255)     gAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACt                                                                                                                                           <  1:200267/150‑1 (MQ=255)           ctgctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGAGAAAAACATGCTCTATCTGCGCAACAGGGTGGCGGCAGGTAGCGAGATCACTTTACTg                                                                                                                                     <  2:605892/150‑1 (MQ=255)               tgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGTCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAg                                                                                                                                 <  1:649980/150‑1 (MQ=255)                   gCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCgct                                                                                                                             <  4:569581/150‑1 (MQ=255)                      ggAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCgctggc                                                                                                                          >  2:521009/1‑150 (MQ=255)                       gAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCgctggct                                                                                                                         >  2:351006/1‑150 (MQ=255)                        aaGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCgaga                                                                                                                                                <  2:1069869/126‑1 (MQ=255)                         aGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCtgt                                                                                                                       <  2:440936/150‑1 (MQ=255)                         aGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCtgt                                                                                                                       >  2:89628/1‑150 (MQ=255)                           aTGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTgc                                                                                                                     <  1:430404/150‑1 (MQ=255)                            tGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTgcc                                                                                                                    >  2:443421/1‑150 (MQ=255)                             ggTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTgccg                                                                                                                   <  1:443421/150‑1 (MQ=255)                                   tGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAg                                                                                                             <  4:567929/150‑1 (MQ=255)                                    gTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgc                                                                                                            <  2:1016101/150‑1 (MQ=255)                                     tttGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgcg                                                                                                           >  3:410755/1‑150 (MQ=255)                                            gATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAcgc                                                                                                    >  4:352975/1‑150 (MQ=255)                                            gATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAcgc                                                                                                    >  1:16587/1‑150 (MQ=255)                                               cAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGAt                                                                                                 <  1:672114/150‑1 (MQ=255)                                                       gggTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGgttgt                                                                                         <  2:3901/150‑1 (MQ=255)                                                           ttCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTAt                                                                                     <  1:714406/150‑1 (MQ=255)                                                             cTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCt                                                                                   <  1:992064/150‑1 (MQ=255)                                                                ttttGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGcc                                                                                <  1:521009/150‑1 (MQ=255)                                                                ttttGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGcc                                                                                >  2:447587/1‑150 (MQ=255)                                                                    gAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGc                                                                            >  1:723110/1‑150 (MQ=255)                                                                     aaGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCa                                                                           >  1:742847/1‑150 (MQ=255)                                                                           gggCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGc                                                                     <  2:130896/150‑1 (MQ=255)                                                                            tgCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGcc                                                                    <  2:1061825/149‑1 (MQ=255)                                                                             gCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAACGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCg                                                                   <  3:375738/150‑1 (MQ=255)                                                                              cGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCgg                                                                  <  3:322623/150‑1 (MQ=255)                                                                              cGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCgg                                                                  >  2:1103302/1‑150 (MQ=255)                                                                               ggATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGa                                                                 <  2:522488/150‑1 (MQ=255)                                                                               ggATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGa                                                                 >  2:222683/1‑150 (MQ=255)                                                                                     agatcgacacGTCCGCGCGACCAGCCTGGAAACTCTGCGCACCATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCGGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCAcg                                                           <  4:640435/144‑1 (MQ=255)                                                                                     aGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCAcg                                                           >  1:900500/1‑150 (MQ=255)                                                                                     aGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCAGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCAcg                                                           >  3:395046/1‑150 (MQ=255)                                                                                         acacacTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCAcgccgc                                                       >  4:221363/1‑150 (MQ=255)                                                                                          cacacTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCg                                                                               >  1:853082/1‑125 (MQ=255)                                                                                          cacacTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCg                                                                               <  2:853082/125‑1 (MQ=255)                                                                                          aacaaTTGCGGGCGACGAGGCAGGGAAAGCTGCGGAATAGGGTGGCGGCAGGTAGCGAGATCACTTTACTGCAAGCTCTGGCTGTGCCACCGCAGCGAAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGACGGAACCACGCCGCa                                                      <  2:235399/149‑1 (MQ=255)                                                                                           acacTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCGGCCGGAGCGCTAACGCGAGGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCAc                                                     >  1:45311/1‑150 (MQ=255)                                                                                           acacTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCAc                                                     >  2:971925/1‑150 (MQ=255)                                                                                               ttCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGGGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTAtt                                                 >  1:679478/1‑150 (MQ=255)                                                                                               ttCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGAt                                                                                                 >  4:424418/1‑102 (MQ=255)                                                                                               ttCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGAt                                                                                                 <  3:424418/102‑1 (MQ=255)                                                                                               ttCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTAtt                                                 >  2:702201/1‑150 (MQ=255)                                                                                                  cgcgcgACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGc                                              >  4:5293/1‑150 (MQ=255)                                                                                                   gcgcgACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGcc                                             <  1:89628/150‑1 (MQ=255)                                                                                                         ccAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGttt                                       <  1:351006/150‑1 (MQ=255)                                                                                                          cAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTa                                      >  3:366489/1‑150 (MQ=255)                                                                                                          cAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTa                                      <  1:971925/150‑1 (MQ=255)                                                                                                            gCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCaa                                                                                                        >  2:769854/1‑82 (MQ=255)                                                                                                            gCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCaa                                                                                                        <  1:769854/82‑1 (MQ=255)                                                                                                            gCCCGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGATCCACGCCGCACTATTGGCCTGGTCTATc                                    <  2:613427/150‑1 (MQ=255)                                                                                                               tGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTc                                 >  3:264750/1‑150 (MQ=255)                                                                                                                 gAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAGCCACGCCGCACTATTGGCCTGGCTTATAGTCCt                               >  1:511008/1‑150 (MQ=255)                                                                                                                 gAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCt                               >  2:10696/1‑150 (MQ=255)                                                                                                                 gAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCt                               <  2:16587/150‑1 (MQ=255)                                                                                                                   aaCTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTgg                             <  3:555153/150‑1 (MQ=255)                                                                                                                   aaCTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTgg                             >  2:527491/1‑150 (MQ=255)                                                                                                                   aaCTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTgg                             <  1:1034428/150‑1 (MQ=255)                                                                                                                   aaCTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTgg                             <  3:196826/150‑1 (MQ=255)                                                                                                                     ctctGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCt                           >  3:14501/1‑150 (MQ=255)                                                                                                                      tctGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTc                          >  2:180548/1‑150 (MQ=255)                                                                                                                          cgcAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCg                      <  1:447587/150‑1 (MQ=255)                                                                                                                               cATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTgcg                 >  2:810017/1‑150 (MQ=255)                                                                                                                                 tggtggCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCa               <  1:10696/150‑1 (MQ=255)                                                                                                                                 tggtggCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGGTTATCGTCCTGGCTCACCGCTGCGCa               >  1:999727/1‑150 (MQ=255)                                                                                                                                   gtggCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGc             <  2:723110/150‑1 (MQ=255)                                                                                                                                   gtggCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGc             >  4:225878/1‑150 (MQ=255)                                                                                                                                      gcggcAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTAt                                                                                     <  3:132277/75‑1 (MQ=255)                                                                                                                                      gcggcAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTAt                                                                                     >  4:132277/1‑75 (MQ=255)                                                                                                                                         gcAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTAt       <  4:272865/150‑1 (MQ=255)                                                                                                                                         gcAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTAt       <  2:545749/150‑1 (MQ=255)                                                                                                                                         gcAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTAt       <  4:410755/150‑1 (MQ=255)                                                                                                                                         gcAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTAt       <  3:352975/150‑1 (MQ=255)                                                                                                                                           aGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGa     <  1:744525/150‑1 (MQ=255)                                                                                                                                           aGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGa     <  4:494509/150‑1 (MQ=255)                                                                                                                                             gTAGCGAGATCACTTGACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTAGCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGagc   <  4:384964/150‑1 (MQ=255)                                                                                                                                              tAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGAAAACAAGATAGGGTAGTTTCAATGCAGTGCATAAAGCCAGAACCACGCCGAACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGagca  <  1:325921/150‑1 (MQ=255)                                                                                                                                               aGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATc                                    <  2:605545/115‑1 (MQ=255)                                                                                                                                               aGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATc                                    >  1:605545/1‑115 (MQ=255)                                                                                                                                                   |                                                                                                                                                 AGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGAGCA  >  NC_000913_3_pae_tpiA/4158966‑4159256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑