Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,158,9840GT51.9% 10.6 / 267.9 155M193I (ATG→ATToxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (38/36);  new base T (43/37);  total (82/73)
Fisher's exact test for biased strand distribution p-value = 8.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 158 total aligned reads displayed.

GTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCT  >  NC_000913_3_pae_tpiA/4158835‑4159133
                                                                                                                                                     |                                                                                                                                                      
  gATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGCGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGGTGGCCGATCTGGCAGGGGAAAAACTGCTGATTCt                                                                                                                                                      <  2:111304/150‑1 (MQ=255)
  gATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCt                                                                                                                                                      <  2:624132/150‑1 (MQ=255)
  gATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCt                                                                                                                                                      <  2:795689/150‑1 (MQ=255)
  gATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGAAGGAGAAAAACTGCTGATTCt                                                                                                                                                      <  4:581736/150‑1 (MQ=255)
     ccTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCGGATTCTGGa                                                                                                                                                   <  1:701796/150‑1 (MQ=255)
      cTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGaa                                                                                                                                                  <  1:751370/150‑1 (MQ=255)
        cgcgCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGa                                                                                                                                                >  1:563133/1‑150 (MQ=255)
          cgcTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATg                                                                                                                                              >  4:342914/1‑150 (MQ=255)
           gcTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATgg                                                                                                                                             >  1:100846/1‑150 (MQ=255)
            cTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTg                                                                                                                                                     <  1:739002/141‑1 (MQ=255)
                    agagagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGttt                                                                                                                                    <  3:136651/150‑1 (MQ=255)
                     gagagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTg                                                                                                                                   >  2:329763/1‑150 (MQ=255)
                     gagagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTg                                                                                                                                   >  2:187375/1‑150 (MQ=255)
                      agagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgc                                                                                                                                  >  3:575740/1‑150 (MQ=255)
                          cGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGa                                                                                                                              >  2:291825/1‑150 (MQ=255)
                           gAAGCATTCATTGAAGTGGCGTTGTTTGATGAGCCTATGTTGCAGGCTATCTATGAAGATCACCCGTGGGAGAACCGCGAATACGTACCTATGGCCGAAAAAACAAAGAAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                                                             <  2:468322/150‑1 (MQ=255)
                               cattcattGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAgg                                                                                                                         <  3:643353/150‑1 (MQ=255)
                                           tGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCt                                                                                                             >  2:640211/1‑150 (MQ=255)
                                           tGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCt                                                                                                             <  4:145387/150‑1 (MQ=255)
                                              cGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtt                                                                                                          >  3:100404/1‑150 (MQ=255)
                                              cGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtt                                                                                                          >  2:268272/1‑150 (MQ=255)
                                               gttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGttt                                                                                                         >  3:78254/1‑150 (MQ=255)
                                                ttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtttt                                                                                                        <  3:332951/150‑1 (MQ=255)
                                                ttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtttt                                                                                                        <  2:100846/150‑1 (MQ=255)
                                                   tttGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGTGCTGTCAGGGGAAAAACTGCTGATCCTGGAAGATGGTCACTGTTTGCGCGATCACGCAATGGGTTTCTGTTTTGaa                                                                                                     >  4:187242/1‑150 (MQ=255)
                                                     tgatgaGCCAATGTTGCTGTCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGTCAGGGGAAAAACTGGGGATGCTGGGAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGc                                                                                                   >  1:776702/1‑150 (MQ=255)
                                                      gatgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGcc                                                                                                  >  4:58373/1‑150 (MQ=255)
                                                       atgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCg                                                                                                 <  1:640211/150‑1 (MQ=255)
                                                         gaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCggg                                                                                               <  1:110959/150‑1 (MQ=255)
                                                          aGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                                              <  3:525266/150‑1 (MQ=255)
                                                           gCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGc                                                                                             <  4:59942/150‑1 (MQ=255)
                                                            ccAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGa                                                                                                                                                >  2:849647/1‑98 (MQ=255)
                                                            ccAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGa                                                                                                                                                <  1:849647/98‑1 (MQ=255)
                                                            ccAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTg                                                                                                            >  1:936424/1‑134 (MQ=255)
                                                            ccAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTg                                                                                                            <  2:936424/134‑1 (MQ=255)
                                                              aaTGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGa                                                                                          >  2:463351/1‑150 (MQ=255)
                                                               aTGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGaa                                                                                                                                                  <  1:206420/93‑1 (MQ=255)
                                                                tGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATg                                                                                        <  3:392459/150‑1 (MQ=255)
                                                                  ttGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGaa                                                                                      >  2:809679/1‑150 (MQ=255)
                                                                   tGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAg                                                                                     <  4:160442/150‑1 (MQ=255)
                                                                      tGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATa                                                                                  <  1:162114/150‑1 (MQ=255)
                                                                       ggCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATac                                                                                 >  2:308034/1‑150 (MQ=255)
                                                                       ggCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATac                                                                                 >  3:168758/1‑150 (MQ=255)
                                                                          tatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacaca                                                                              >  3:84265/1‑150 (MQ=255)
                                                                              tatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTc                                                                          >  1:1015057/1‑150 (MQ=255)
                                                                               atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTcc                                                                         >  2:218741/1‑150 (MQ=255)
                                                                               atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGACCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTcc                                                                         <  3:342914/150‑1 (MQ=255)
                                                                                tGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg                                                                        >  2:892476/1‑150 (MQ=255)
                                                                                 gAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcgc                                                                      >  4:225041/1‑150 (MQ=255)
                                                                                   aGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGc                                                                                             >  4:328833/1‑126 (MQ=255)
                                                                                         cccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGa                                                                                                      >  4:117501/1‑111 (MQ=255)
                                                                                          ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGc                                                             >  3:615333/1‑150 (MQ=255)
                                                                                           cGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATc                                                                                                                            >  1:408836/1‑87 (MQ=255)
                                                                                           cGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATc                                                                                                                            <  2:408836/87‑1 (MQ=255)
                                                                                               ggCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGa                                                        >  3:580281/1‑150 (MQ=255)
                                                                                                  gAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtttt                                                                                                        >  4:280076/1‑100 (MQ=255)
                                                                                                  gAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtttt                                                                                                        <  3:280076/100‑1 (MQ=255)
                                                                                                   aaCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAct                                                    <  4:1148/150‑1 (MQ=255)
                                                                                                   aaCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATc                                                                                                                            <  1:293933/79‑1 (MQ=255)
                                                                                                   aaCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAct                                                    >  1:1006389/1‑150 (MQ=255)
                                                                                                   aaCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAct                                                    >  1:810631/1‑150 (MQ=255)
                                                                                                    aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAActc                                                   <  1:358046/150‑1 (MQ=255)
                                                                                                    aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAActc                                                   <  3:600999/150‑1 (MQ=255)
                                                                                                      cgcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTg                                                 >  1:902531/1‑150 (MQ=255)
                                                                                                          aaTGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGa                                                                                    >  3:483901/1‑112 (MQ=255)
                                                                                                          aaTGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGa                                                                                    <  4:483901/112‑1 (MQ=255)
                                                                                                               gTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtg                                        >  2:178529/1‑150 (MQ=255)
                                                                                                                tACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg                                       >  1:485032/1‑150 (MQ=255)
                                                                                                                   cGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtgg                                    >  1:283428/1‑150 (MQ=255)
                                                                                                                   cGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtgg                                    <  1:26979/150‑1 (MQ=255)
                                                                                                                   cGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtgg                                    >  2:900356/1‑150 (MQ=255)
                                                                                                                    gATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggc                                   >  3:313017/1‑150 (MQ=255)
                                                                                                                     aTGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtgg                                    >  1:886045/1‑148 (MQ=255)
                                                                                                                        gCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCa                               <  1:329763/150‑1 (MQ=255)
                                                                                                                        gCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCa                               <  1:218741/150‑1 (MQ=255)
                                                                                                                         ccGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAACATACACACTTCCGCGCGACCAGCCCGGAAACTCCGCGCAACATGGTGCCGCCAg                              >  2:785393/1‑150 (MQ=255)
                                                                                                                         ccGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg                              >  1:873053/1‑150 (MQ=255)
                                                                                                                          cGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGCCGGATGCCGATCAGCACTTCGGATGCCGTCGTCTGGATGCTCTGCGCAACATGGTGGCGGCAgg                             >  1:460521/1‑150 (MQ=255)
                                                                                                                            aTCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacac                                                                               <  4:415775/99‑1 (MQ=255)
                                                                                                                            aTCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacac                                                                               >  3:415775/1‑99 (MQ=255)
                                                                                                                              cTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGc                         >  2:490234/1‑150 (MQ=255)
                                                                                                                                   aGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCCAGCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt                    <  3:187242/150‑1 (MQ=255)
                                                                                                                                    ggggAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATc                   <  3:100963/150‑1 (MQ=255)
                                                                                                                                     gggAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCa                  >  4:551970/1‑150 (MQ=255)
                                                                                                                                       gAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACt                >  4:408103/1‑150 (MQ=255)
                                                                                                                                       gAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACt                >  2:360382/1‑150 (MQ=255)
                                                                                                                                        aaaaaCTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGATTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACtt               >  3:441570/1‑150 (MQ=255)
                                                                                                                                          aaaCTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTa             <  2:935340/150‑1 (MQ=255)
                                                                                                                                          aaaCTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTa             >  3:245833/1‑150 (MQ=255)
                                                                                                                                                ctgATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCa       >  1:215095/1‑150 (MQ=255)
                                                                                                                                                ctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACATTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCa       >  2:589426/1‑150 (MQ=255)
                                                                                                                                                 tgATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAg      <  1:360382/150‑1 (MQ=255)
                                                                                                                                                 tgATTCTGGAAGATGGTCACTGTTGGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAATCTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAg      <  2:348362/150‑1 (MQ=255)
                                                                                                                                                  gATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgg                                                                                                <  4:529860/60‑1 (MQ=255)
                                                                                                                                                  gATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAgg                             <  1:171297/126‑1 (MQ=255)
                                                                                                                                                   aTGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAgcg    <  2:776702/150‑1 (MQ=255)
                                                                                                                                                    tGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAgcgc   <  2:1034072/150‑1 (MQ=255)
                                                                                                                                                    tGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAgcgc   >  1:993417/1‑150 (MQ=255)
                                                                                                                                                     gCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgct  >  1:293353/1‑150 (MQ=255)
                                                                                                                                                     gCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgct  >  2:503449/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCT  >  NC_000913_3_pae_tpiA/4158835‑4159133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: