Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,313,049	C→T	intergenic (‑142/‑217)	alsR ← / → rpiB	d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR/ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,313,049	0	C	T	100.0%	299.4 / NA	82	intergenic (‑142/‑217)	alsR/rpiB	d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR/ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/41);  total (41/41)

CTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTT  >  NC_000913_3_pae_tpiA/4312903‑4313195                                                                                                                                                   |                                                                                                                                                   cTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTttattttatt                                                                                                                                                 <  2:121207/150‑1 (MQ=255) cTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTttattttatt                                                                                                                                                 <  2:159197/150‑1 (MQ=255)  tcatcaCGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTtattttattt                                                                                                                                                >  2:90565/1‑150 (MQ=255)  tcatcaCGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTtattttattt                                                                                                                                                >  1:423443/1‑150 (MQ=255)    atcaCGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTaa                                                                                                                                              >  1:625472/1‑150 (MQ=255)       aCGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTa                                                                                                                                           >  1:424303/1‑150 (MQ=255)          ttCTCTTGTCCTTGAATTGAAAACGCTACTACATCACCACAGAAAGAACACAAAAGATGCTGGCGAAAAAAAAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGtt                                                                                                                                        <  2:499683/150‑1 (MQ=255)                 gTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAAc                                                                                                                                 <  1:923332/150‑1 (MQ=255)                    cTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACatg                                                                                                                              >  3:414077/1‑150 (MQ=255)                     ttgaattgaaGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACatga                                                                                                                             >  4:340376/1‑150 (MQ=255)                      tgaattgaaGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACatgat                                                                                                                            <  1:886174/150‑1 (MQ=255)                       gacttaaaGACGCCACCATATCACCACCGCCAGACCAGCAAAGATGCTGGCCACTTCACAACAAAATAACACGTATAAAGCTTATTTTATTGTGAACAATGGCGGGCGGCTTAAAATTTTATGTTATGTATTCAGTTTTCACACatgctg                                                                                                                           >  2:209411/7‑150 (MQ=255)                       gaattgaaGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACatgatg                                                                                                                           <  2:443472/150‑1 (MQ=255)                         attgaaGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGtt                                                                                                                         >  3:456929/1‑150 (MQ=255)                           tgaaGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTAt                                                                                                                       >  1:82750/1‑150 (MQ=255)                             aaGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAACACATAAACTTTATATAGTTCATTTGATTGTTAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTAttt                                                                                                                     <  1:205888/150‑1 (MQ=255)                                    cACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTc                                                                                                              >  1:22324/1‑150 (MQ=255)                                     aCTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCt                                                                                                             >  2:124462/1‑150 (MQ=255)                                     aCTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCt                                                                                                             <  1:761527/150‑1 (MQ=255)                                     aCTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCt                                                                                                             <  4:552240/150‑1 (MQ=255)                                     aCTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCt                                                                                                             <  2:362298/150‑1 (MQ=255)                                     aCTATATCAACACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCt                                                                                                             <  2:362295/150‑1 (MQ=255)                                       tataTCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTAtt                                                                                                                      <  1:195615/139‑1 (MQ=255)                                       tataTCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTAtt                                                                                                                      >  2:195615/1‑139 (MQ=255)                                         tatCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGtt                                                                                                         >  2:111428/1‑150 (MQ=255)                                               cacaGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAg                                                                                                   >  3:212750/1‑150 (MQ=255)                                                  aGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAgatg                                                                                                >  1:484745/1‑150 (MQ=255)                                                  aGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAgatg                                                                                                >  2:357555/1‑150 (MQ=255)                                                    cAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAgatgat                                                                                              >  3:44111/1‑150 (MQ=255)                                                     aaGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATa                                                                                             >  2:749004/1‑150 (MQ=255)                                                           aGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGCGGCTTAAAATTTTATTGTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGtt                                                                                       >  1:430271/1‑150 (MQ=255)                                                            gAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTg                                                                                      >  4:242058/1‑150 (MQ=255)                                                             aaaaGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGc                                                                                     <  3:58028/150‑1 (MQ=255)                                                                 gATGCTGGAGAATTAACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGa                                                                                 <  2:843871/150‑1 (MQ=255)                                                                   tGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATg                                                                               <  2:82750/150‑1 (MQ=255)                                                                   tGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATg                                                                               <  2:755359/150‑1 (MQ=255)                                                                    gCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATgg                                                                              >  1:972832/1‑150 (MQ=255)                                                                     cTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGt                                                                             <  1:124462/150‑1 (MQ=255)                                                                      tGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTg                                                                            <  2:476798/150‑1 (MQ=255)                                                                         cGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAgt                                                                         <  2:22324/150‑1 (MQ=255)                                                                           aCTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAgtgt                                                                       >  4:167769/1‑150 (MQ=255)                                                                             ttCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGAt                                                                                          >  2:922383/1‑129 (MQ=255)                                                                             ttCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGAt                                                                                          <  1:922383/129‑1 (MQ=255)                                                                             ttCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCt                                                                     <  2:972832/150‑1 (MQ=255)                                                                             ttCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCt                                                                     >  2:399757/1‑150 (MQ=255)                                                                              tCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCtt                                                                    >  2:884835/1‑150 (MQ=255)                                                                                  aaaaaaaTAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAAc                                                                >  2:81655/1‑150 (MQ=255)                                                                                   aaaaaaTAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACg                                                               >  1:843254/1‑150 (MQ=255)                                                                                   aaaaaaTAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACg                                                               >  3:283017/1‑150 (MQ=255)                                                                                     aaaataaaatTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCt                                                             >  2:414963/1‑150 (MQ=255)                                                                                       aataaaatTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTAt                                                           >  2:700594/1‑150 (MQ=255)                                                                                         taaaatTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTg                                                         >  1:237300/1‑150 (MQ=255)                                                                                          aaaatTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATATATGTTGCTGTATGGTGCGTGTCTTTAACGCTAtttg                                                        >  4:305993/1‑148 (MQ=255)                                                                                             atTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCt                                                     >  2:545601/1‑150 (MQ=255)                                                                                             atTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCt                                                     <  2:898137/150‑1 (MQ=255)                                                                                                 ataAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCa                                                 <  1:399757/150‑1 (MQ=255)                                                                                                 ataAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCa                                                 <  1:357555/150‑1 (MQ=255)                                                                                                  taAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAg                                                <  2:423443/150‑1 (MQ=255)                                                                                                   aaaGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGa                                               <  1:936423/150‑1 (MQ=255)                                                                                                   aaaGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGa                                               <  3:410234/150‑1 (MQ=255)                                                                                                    taGTTTATTTTATTGTGAACAATGTCTAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGAt                                              <  4:485445/149‑1 (MQ=255)                                                                                                      gTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGt                                            <  2:237300/150‑1 (MQ=255)                                                                                                        ttattttattGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTaa                                          <  2:865065/150‑1 (MQ=255)                                                                                                        ttattttattGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTaa                                          <  2:999286/150‑1 (MQ=255)                                                                                                          attttattGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAAct                                        <  2:735012/150‑1 (MQ=255)                                                                                                           ttttattGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAActc                                       >  1:253251/1‑150 (MQ=255)                                                                                                           ttttattGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTATTTAACGCTATTTAGCTGTCAGATGTAActc                                       >  3:33214/1‑150 (MQ=255)                                                                                                            tttattGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAActct                                      <  4:283017/150‑1 (MQ=255)                                                                                                            tttattGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAActct                                      <  3:340376/150‑1 (MQ=255)                                                                                                              tattGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGt                                    <  2:216165/150‑1 (MQ=255)                                                                                                              tattGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGt                                    <  4:426760/150‑1 (MQ=255)                                                                                                               attGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAg                                                                                                   >  2:396736/1‑86 (MQ=255)                                                                                                               attGTGAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAg                                                                                                   <  1:396736/86‑1 (MQ=255)                                                                                                                  gtgAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTaaaa                                >  4:291353/1‑150 (MQ=255)                                                                                                                   tgAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAAc                               >  1:892712/1‑150 (MQ=255)                                                                                                                    gAACAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTTAAACa                              >  1:220469/1‑150 (MQ=255)                                                                                                                       cAATGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGAt                           <  1:90565/150‑1 (MQ=255)                                                                                                                        tatagCGAGTTTCTTAAAATGTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGCTGTTGGCTGTTGCTGGGGGGTGGGTGTCGTTAACGCTATGGCGCTGTCAGATGTACCTCTGTAAAACAgctt                          <  2:727413/146‑4 (MQ=255)                                                                                                                        aaTGGCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTTTATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATc                          >  2:632439/1‑150 (MQ=255)                                                                                                                         aTGGCGAGTGTCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTGTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCa                         <  4:591180/150‑1 (MQ=255)                                                                                                                           ggCGAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAgg                       >  3:257662/1‑150 (MQ=255)                                                                                                                              gattGGCTGAAAATTGTATTCTATTTAATGAGGGTAAAAGCATGATGTTATTTTTTTTTCTGCTGTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAg                    <  1:209411/147‑1 (MQ=255)                                                                                                                              gAGTGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAg                    <  2:253251/150‑1 (MQ=255)                                                                                                                                 tGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATc                          >  3:65363/1‑141 (MQ=255)                                                                                                                                 tGGCTTAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATc                          <  4:65363/141‑1 (MQ=255)                                                                                                                                      tAAAATTTTATTTTATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAGCTCTGTAAAACAGATCAGGAAGGCGTAAtt            >  1:222745/1‑150 (MQ=255)                                                                                                                                           ttttattttaTTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCg       >  1:354282/1‑150 (MQ=255)                                                                                                                                                ttttATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGAtttt  >  3:504024/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTT  >  NC_000913_3_pae_tpiA/4312903‑4313195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑