Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	2,740,078	T→C	intergenic (‑9/+2)	tyrA ← / ← aroF	fused chorismate mutase T/prephenate dehydrogenase/3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	2,740,078	0	T	C	100.0%	231.5 / NA	65	intergenic (‑9/+2)	tyrA/aroF	fused chorismate mutase T/prephenate dehydrogenase/3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/28);  total (37/28)

AGTCCAAAGCGGCTTTTCACCTCGCCCACTTCAGCAACCAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCTCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTATTTCATTTCACTGCGCGGTTGCTCGGAAGACTGATTGCCC  >  NC_000913_3_pae_tpiA/2739945‑2740227                                                                                                                                      |                                                                                                                                                      aGTCCAAAGCGGCTTTTCACCTCGCCCACTTCAGCAACCAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGc                                                                                                                                       >  4:511574/1‑150 (MQ=255)  gTCCAAAGCGGCTTTTCACCTCGCCCACTTCAGCAACCAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGcc                                                                                                                                      >  1:730994/1‑150 (MQ=255)  gTCCAAAGCGGCTTTTCACCTCGCCCACTTCAGCAACCAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGcc                                                                                                                                      <  2:548837/150‑1 (MQ=255)   tCCAAAGCGGCTTTTCACCTCGCCCACTTCAGCAACCAGTTCCAGACTCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCAGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCg                                                                                                                                     <  2:567156/150‑1 (MQ=255)   tCCAAAGCGGCTTTTCACCTCGCCCACTTCAGCAACCAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCg                                                                                                                                     >  1:67947/1‑150 (MQ=255)       aaGCGGCTTTTCACCTCGCCCACTTCATCAACCAGTTCCAGACGCTGCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAg                                                                                                                                 <  1:241517/150‑1 (MQ=255)         gCGGCTTTTCACCTCGCCCACTTCAGCAACCAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGTGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCt                                                                                                                               >  3:177337/1‑150 (MQ=255)                ttCACCTCGCCCACTTCAGCAACCAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGtt                                                                                                                        <  1:500022/150‑1 (MQ=255)                   aCCTCGCCCACTTCAGCAACCAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAg                                                                                                                     >  2:712613/1‑150 (MQ=255)                      tCGCCCACTTCAGCAACCAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATc                                                                                                                  >  4:20587/1‑150 (MQ=255)                      tCGCCCACTTCAGCAACCAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTCCAGATc                                                                                                                  >  1:479405/1‑150 (MQ=255)                       cGCCCACTTCAGCAACCAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATcc                                                                                                                 >  3:484515/1‑150 (MQ=255)                       cGCCCACTTCAGCAACCAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATcc                                                                                                                 <  3:507377/150‑1 (MQ=255)                        gCCCACTTCAGCAACCAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCt                                                                                                                >  2:694677/1‑150 (MQ=255)                                       cAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACgca                                                                                                 <  3:426970/150‑1 (MQ=255)                                        aGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCCGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACgcag                                                                                                >  3:1964/1‑150 (MQ=255)                                         gTTCCAGACGCTTCGCTAATAAATTAAGCAGCGCTTTATCGACTTCATCAATTTGATCGAGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACgcagc                                                                                               <  1:208342/150‑1 (MQ=255)                                            ccAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAg                                                                                            >  1:317820/1‑150 (MQ=255)                                               gACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCa                                                                                         >  4:191087/1‑150 (MQ=255)                                                aCGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCAt                                                                                        >  3:131655/1‑150 (MQ=255)                                                      cGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCa                                                                                  <  1:712613/150‑1 (MQ=255)                                                      cGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATCCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCa                                                                                  <  2:57612/150‑1 (MQ=255)                                                       gCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCAt                                                                                 >  4:327740/1‑150 (MQ=255)                                                        cTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGAACCAAGGCATCGGTCAtt                                                                                >  1:672186/1‑150 (MQ=255)                                                         taataaATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCAttt                                                                               >  4:135528/1‑150 (MQ=255)                                                         taataaATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCAttt                                                                               >  2:806795/1‑150 (MQ=255)                                                           ataaATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCTGGCAATTCCGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTcc                                                                             >  1:153291/1‑150 (MQ=255)                                                             aaaTTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCa                                                                           <  4:279160/150‑1 (MQ=255)                                                               aTTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGc                                                                         >  1:74357/1‑150 (MQ=255)                                                                    gcagcGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTCCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATg                                                                    <  2:479405/150‑1 (MQ=255)                                                                     cagcGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGc                                                                   <  3:191087/150‑1 (MQ=255)                                                                       gcgcTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATCCACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAg                                                                 <  4:36634/150‑1 (MQ=255)                                                                        cgcTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAgg                                                                >  1:439901/1‑150 (MQ=255)                                                                               tCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGt                                                         >  1:90732/1‑150 (MQ=255)                                                                                 gACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTa                                                       >  2:518732/1‑150 (MQ=255)                                                                                  aCTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTaa                                                                                                                                                  <  4:250493/58‑1 (MQ=255)                                                                                  aCTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTaa                                                                                                                                                  >  3:250493/1‑58 (MQ=255)                                                                                     tcatcaATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATcc                                                                                                                 >  1:638534/1‑88 (MQ=255)                                                                                     tcatcaATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATcc                                                                                                                 <  2:638534/88‑1 (MQ=255)                                                                                     tcatcaATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTc                                                                                   >  2:27777/1‑118 (MQ=255)                                                                                     tcatcaATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTc                                                                                   <  1:27777/118‑1 (MQ=255)                                                                                       atcaATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATa                                                 >  4:372136/1‑150 (MQ=255)                                                                                       atcaATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATa                                                 >  1:536003/1‑150 (MQ=255)                                                                                        tcaATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGt                                                                                    >  1:61361/1‑114 (MQ=255)                                                                                        tcaATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGt                                                                                    <  2:61361/114‑1 (MQ=255)                                                                                          aaTTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGCTCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATacac                                              >  1:32253/1‑150 (MQ=255)                                                                                          aaTTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATacac                                              <  1:469875/150‑1 (MQ=255)                                                                                          aaTTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATacac                                              >  2:122445/1‑150 (MQ=255)                                                                                                 tCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTattt                                       >  4:327559/1‑150 (MQ=255)                                                                                                     gTAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTatttcatt                                   <  2:730994/150‑1 (MQ=255)                                                                                                     gTAATGCGGTCAATGCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTatttcatt                                   <  2:665708/150‑1 (MQ=255)                                                                                                      tAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCa                                                                                                     >  1:528155/1‑83 (MQ=255)                                                                                                      tAATGCGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCa                                                                                                     <  2:528155/83‑1 (MQ=255)                                                                                                           cGGTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTATTTCATTTCACTg                             >  3:220841/1‑150 (MQ=255)                                                                                                             gTCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTATTTCATTTCACTgcg                           >  2:757356/1‑150 (MQ=255)                                                                                                              tCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCt                                                                                                                >  1:834867/1‑64 (MQ=255)                                                                                                              tCAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCt                                                                                                                <  2:834867/64‑1 (MQ=255)                                                                                                               cAATTCAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCTCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTATTTCGTTTCACTGcgcg                         <  4:449619/150‑1 (MQ=255)                                                                                                                    cAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTatttca                                     <  3:328389/133‑1 (MQ=255)                                                                                                                    cAGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTatttca                                     >  4:328389/1‑133 (MQ=255)                                                                                                                     aGCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTATTTCATTTCACTGCGCGGTTGCt                   <  4:484515/150‑1 (MQ=255)                                                                                                                      gCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTATTTCATTTCACTGCGCGGTTGCTc                  <  4:177337/150‑1 (MQ=255)                                                                                                                      gCAACCATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTATTTCATTTCACTGCGCGGTTGCTc                  <  2:90732/150‑1 (MQ=255)                                                                                                                          ccATAATAAACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTATTTCATTTCACTGCGCGGTTGCTCGGaa              >  1:366301/1‑150 (MQ=255)                                                                                                                                taaACCCCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTATTTCATTTCACTGCGCGGTTGCTCGGAAGACTGa        <  2:224577/150‑1 (MQ=255)                                                                                                                                      tctTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTATTTCATTTCACTGCGCGGTTGCTCGGAAGACTGATTGccc  >  2:597305/1‑150 (MQ=255)                                                                                                                                      |                                                                                                                                                      AGTCCAAAGCGGCTTTTCACCTCGCCCACTTCAGCAACCAGTTCCAGACGCTTCGCTAATAAATTCAGCAGCGCTTTATCGACTTCATCAATTTGATCGCGTAATGCGGTCAATTCAGCAACCATAATAAACCTCTTAAGCCACGCGAGCCGTCAGCTGCCCGTTCAGATCCTGATGAATTTCACGCAGCAAGGCATCGGTCATTTCCCAGCTAATGCAGGCATCGGTTACGGATACACCGTATTTCATTTCACTGCGCGGTTGCTCGGAAGACTGATTGCCC  >  NC_000913_3_pae_tpiA/2739945‑2740227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑