Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	3,075,359	T→C	N275S (AAC→AGC)	yggF ←	fructose 1,6 bisphosphatase isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	3,075,359	0	T	C	99.0%	330.6 / ‑4.5	95	N275S (AAC→AGC)	yggF	fructose 1,6 bisphosphatase isozyme
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (53/41);  total (53/42)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCAT  >  NC_000913_3_pae_tpiA/3075212‑3075504                                                                                                                                                    |                                                                                                                                                  cACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGAATGTCGCTc                                                                                                                                                 >  2:658272/1‑150 (MQ=255)        aaTGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCa                                                                                                                                          >  2:160629/1‑150 (MQ=255)         aTGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCAc                                                                                                                                         >  2:810266/1‑150 (MQ=255)         aTGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCAc                                                                                                                                         <  1:40741/150‑1 (MQ=255)           gCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCa                                                                                                                                       <  3:106844/150‑1 (MQ=255)                   tCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATcg                                                                                                                               >  1:580728/1‑150 (MQ=255)                    cTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATcga                                                                                                                              <  3:471323/150‑1 (MQ=255)                      attatattaCACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATcgagc                                                                                                                            <  2:196907/150‑1 (MQ=255)                        tatattaCACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATcgagcga                                                                                                                          <  2:408796/150‑1 (MQ=255)                          tattaCACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGt                                                                                                                        <  4:342691/150‑1 (MQ=255)                            ttaCACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTCCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTACCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTaa                                                                                                                      >  2:712786/1‑149 (MQ=255)                                acGTTTGGTCTGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGGCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTacacac                                                                                                                  >  2:123868/1‑150 (MQ=255)                                 cGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACg                                                                                                                 <  2:140400/150‑1 (MQ=255)                                  gTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACgg                                                                                                                >  2:20895/1‑150 (MQ=255)                                  gTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTCCATCGAGCGAGTACACACgg                                                                                                                >  4:250486/1‑150 (MQ=255)                                     tGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTa                                                                                                             <  3:224826/150‑1 (MQ=255)                                      ggTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTaa                                                                                                            <  3:335801/150‑1 (MQ=255)                                       gTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAAc                                                                                                           <  1:129871/150‑1 (MQ=255)                                          cgcgCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGt                                                                                                                                     <  2:678460/121‑1 (MQ=255)                                          cgcgCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGt                                                                                                                                     >  1:678460/1‑121 (MQ=255)                                                  cgATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCa                                                                                                >  1:235825/1‑150 (MQ=255)                                                    aTCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCAtt                                                                                              <  1:106291/150‑1 (MQ=255)                                                    aTCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCAtt                                                                                              >  3:460837/1‑150 (MQ=255)                                                    aTCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCAtt                                                                                              <  3:190273/150‑1 (MQ=255)                                                         tAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTGCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGGGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCtt                                                                                         <  2:277054/150‑1 (MQ=255)                                                            tGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCa                                                                                      <  3:409794/150‑1 (MQ=255)                                                             gTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCaa                                                                                     >  1:350486/1‑150 (MQ=255)                                                              tCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAAc                                                                                    >  2:342389/1‑150 (MQ=255)                                                                tGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGc                                                                                  >  2:280841/1‑150 (MQ=255)                                                                tGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGc                                                                                  >  1:760856/1‑150 (MQ=255)                                                                tGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGc                                                                                  >  2:95518/1‑150 (MQ=255)                                                                  cGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCGGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCtt                                                                                <  2:575213/150‑1 (MQ=255)                                                                    tCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGTGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTgc                                                                              >  1:712465/1‑150 (MQ=255)                                                                      cGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATcgagcga                                                                                                                          <  1:54006/104‑1 (MQ=255)                                                                      cGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATcgagcga                                                                                                                          >  2:54006/1‑104 (MQ=255)                                                                      cGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTgcgc                                                                            >  1:337672/1‑150 (MQ=255)                                                                        cACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTcc                                                                                                                                                <  1:50824/80‑1 (MQ=255)                                                                        cACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTcc                                                                                                                                                >  2:50824/1‑80 (MQ=255)                                                                        cACCCCATTCGCCGTCTGTTGAAGACCGGTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATGGCCTTGCAACGCGTGCGCTc                                                                          >  2:496480/1‑150 (MQ=255)                                                                         aCCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAAc                                                                                                           >  2:100631/1‑116 (MQ=255)                                                                         aCCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAAc                                                                                                           <  1:100631/116‑1 (MQ=255)                                                                          ccccATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCt                                                                        <  2:566596/150‑1 (MQ=255)                                                                          ccccATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCt                                                                        >  3:456610/1‑150 (MQ=255)                                                                          ccccATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCt                                                                        >  3:456665/1‑150 (MQ=255)                                                                           cccATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGcc                                                                                                  >  2:445016/1‑123 (MQ=255)                                                                           cccATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGcc                                                                                                  <  1:445016/123‑1 (MQ=255)                                                                           cccATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCAtt                                                                                              <  1:251792/127‑1 (MQ=255)                                                                           cccATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCAtt                                                                                              <  1:164172/127‑1 (MQ=255)                                                                           cccATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCAtt                                                                                              >  2:164172/1‑127 (MQ=255)                                                                           cccATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCAtt                                                                                              >  2:251792/1‑127 (MQ=255)                                                                           cccATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTg                                                                       >  2:472158/1‑150 (MQ=255)                                                                           cccATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTg                                                                       >  1:151981/1‑150 (MQ=255)                                                                             cATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGct                                                                     >  2:685717/1‑150 (MQ=255)                                                                                   ccGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCaa                                                               >  4:150639/1‑150 (MQ=255)                                                                                        tGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGc                                                          >  4:269998/1‑150 (MQ=255)                                                                                        tGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGc                                                          <  1:160629/150‑1 (MQ=255)                                                                                         gTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGt                                                                                                                        >  4:505983/1‑87 (MQ=255)                                                                                         gTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGt                                                                                                                        <  3:505983/87‑1 (MQ=255)                                                                                          ttGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCg                                                        >  3:191824/1‑150 (MQ=255)                                                                                           tGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGt                                                                                                               >  2:216736/1‑94 (MQ=255)                                                                                           tGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGt                                                                                                               <  1:216736/94‑1 (MQ=255)                                                                                              aTACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGAttt                                                    <  1:342389/150‑1 (MQ=255)                                                                                               tACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGAtttt                                                   >  2:687964/1‑150 (MQ=255)                                                                                                  cGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTacacac                                                                                                                  >  3:400973/1‑84 (MQ=255)                                                                                                  cGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCg                                                >  3:61650/1‑150 (MQ=255)                                                                                                  cGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGCacacac                                                                                                                  <  4:400973/84‑1 (MQ=255)                                                                                                   gTTCACCAGCTCACCGCCCGTCACGTCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCgt                                               <  2:579266/150‑1 (MQ=255)                                                                                                    ttCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCgtg                                              <  3:150639/150‑1 (MQ=255)                                                                                                     tCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCgtgt                                             >  4:464970/1‑150 (MQ=255)                                                                                                         cAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATc                                         >  1:334316/1‑150 (MQ=255)                                                                                                          aGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATcc                                        >  4:513153/1‑150 (MQ=255)                                                                                                           gCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATccc                                       <  2:715277/150‑1 (MQ=255)                                                                                                            cTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATcccc                                      >  1:484284/1‑150 (MQ=255)                                                                                                             tCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCt                                     >  3:211798/1‑150 (MQ=255)                                                                                                              cACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCtt                                    <  3:250486/150‑1 (MQ=255)                                                                                                               aCCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCttt                                   >  1:301644/1‑150 (MQ=255)                                                                                                                  gCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGc                                <  4:191824/150‑1 (MQ=255)                                                                                                                   cccGTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGcc                               <  2:580728/150‑1 (MQ=255)                                                                                                                      gTCACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTgg                            <  4:325751/150‑1 (MQ=255)                                                                                                                        cACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGctct                                                                   <  3:173222/109‑1 (MQ=255)                                                                                                                        cACGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGctct                                                                   >  4:173222/1‑109 (MQ=255)                                                                                                                         aCGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCAt                                                                                               <  1:367796/80‑1 (MQ=255)                                                                                                                         aCGCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCAt                                                                                               >  2:367796/1‑80 (MQ=255)                                                                                                                           gCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTACCGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCaaaa                       >  3:119115/1‑150 (MQ=255)                                                                                                                           gCCCGTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCaaaa                       <  1:472158/150‑1 (MQ=255)                                                                                                                               gTAGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCa                   <  2:745192/150‑1 (MQ=255)                                                                                                                                gaGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCAGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGTAAAAGTCaa                  <  2:687820/149‑1 (MQ=255)                                                                                                                                 aGCGCTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAAt                 >  4:342286/1‑150 (MQ=255)                                                                                                                                    gcTAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAg              >  3:187190/1‑150 (MQ=255)                                                                                                                                      tAAAGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCt            >  1:527125/1‑150 (MQ=255)                                                                                                                                      tAAAGAGGATATCGCTCCCCCTCAACAAACCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCt            >  2:626862/1‑150 (MQ=255)                                                                                                                                        aaGAGGATATCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCg          >  2:529895/1‑150 (MQ=255)                                                                                                                                                atCGCTCCCCCTCACCAGTTCATCGAGCGAGTATACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTg                                                                  >  4:219715/1‑86 (MQ=255)                                                                                                                                                atCGCTCCCCCTCACCAGTTCATCGAGCGAGTATACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTg                                                                  <  3:219715/86‑1 (MQ=255)                                                                                                                                                atCGCTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCat  <  1:498423/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCAT  >  NC_000913_3_pae_tpiA/3075212‑3075504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑