New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	2530197 =	29 (1.170)	3 (0.130)	3/268	3.1	9.2%	intergenic (‑21/+50)	ligA/zipA	DNA ligase, NAD(+)‑dependent/FtsZ stabilizer
	?	NC_000913_3_pae_tpiA	2530218 =	32 (1.370)					intergenic (‑42/+29)	ligA/zipA	DNA ligase, NAD(+)‑dependent/FtsZ stabilizer
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACAC  >  NC_000913_3_pae_tpiA/2530060‑2530341                                                                                                                                          |                                                                                                                                                 cATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACaa                                                                                                                                       <  1:122489/149‑1 (MQ=255)          gTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGtt                                                                                                                              >  1:300809/1‑149 (MQ=255)           tCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTc                                                                                                                             >  1:420607/1‑149 (MQ=255)                   aGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAgagga                                                                                                                     >  2:12589/1‑149 (MQ=255)                   aGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAgagga                                                                                                                     <  1:361051/149‑1 (MQ=255)                       acGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGTCAAGCGGGGGTTCGAAGAGGAGTTa                                                                                                                 >  2:328430/2‑149 (MQ=255)                                    gcgcATCCATCACATGATAAAGTTATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAgt                                                                                                    <  1:63372/149‑1 (MQ=255)                                           cgtccactGATAAAGTTACTCATGATGGCGAAGCGTCGTTCGCAGGTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAg                                                                                             <  2:327211/142‑1 (MQ=255)                                           catcACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGGGTTATTTTGCCTTAAGTGTATCAg                                                                                             >  1:267280/1‑149 (MQ=255)                                            atcACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAgg                                                                                            <  2:271385/149‑1 (MQ=255)                                               acaTGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCAACAAGCGGGGCTTCAAAGAGGAGTTAATTTGCCTTCAGTGTATCAAGCGt                                                                                         >  1:52340/1‑149 (MQ=255)                                                 aTGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTg                                                                                       >  1:352983/1‑149 (MQ=255)                                                 aTGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTg                                                                                       <  1:12589/149‑1 (MQ=255)                                                     tAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGt                                                                                   <  1:196983/149‑1 (MQ=255)                                                         gATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCttt                                                                               <  2:331061/149‑1 (MQ=255)                                                         gATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCttt                                                                               <  2:136444/149‑1 (MQ=255)                                                                     ggCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGgat                                                                   <  2:300809/149‑1 (MQ=255)                                                                              tcgtTCGCAGTTCTGTCTGTGGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGTTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTg                                                          <  2:278831/149‑1 (MQ=255)                                                                              tcgtTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTGATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTg                                                          <  1:120741/149‑1 (MQ=255)                                                                                   cGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACt                                                     <  2:352983/149‑1 (MQ=255)                                                                                    gCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCCGGCGCATTCCGCTTGGACTTCGCGGATGATGTCCTGGTACTc                                                    <  1:416993/149‑1 (MQ=255)                                                                                                       ttCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCttt                                                                               >  2:328499/1‑103 (MQ=255)                                                                                                       ttCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCttt                                                                               <  1:328499/103‑1 (MQ=255)                                                                                                                   cATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGGGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGgcgc                     >  1:397485/1‑149 (MQ=255)                                                                                                                    atatCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCt                    >  1:138507/1‑149 (MQ=255)                                                                                                                               tCACTGCTAAAAACCCCCGACAAGCGGGGGGTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGc         <  1:125192/149‑1 (MQ=255)                                                                                                                                 aaTGCTAAAACCCCCCAACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCAc       <  2:264130/149‑1 (MQ=255)                                                                                                                                   tGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGa     <  2:220622/149‑1 (MQ=255)                                                                                                                                      tAAAAACCCCCGACAAGCGGGGGTGCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAAGCGTTTGCGTATTTGACTTCGCGGATGATGTACTGGTACTCGCGCAATTTCTGCGTAGCCATCATGCCGCACTGCTCGTCCAGCACGccac  >  2:185056/1‑149 (MQ=255)                                                                                                                                          |                                                                                                                                                 CATCAGCCTGTCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACAC  >  NC_000913_3_pae_tpiA/2530060‑2530341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.