Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	2,795,396	C→A	Q382K (CAG→AAG)	gabP →	gamma‑aminobutyrate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	2,795,396	0	C	A	100.0%	81.1 / NA	27	Q382K (CAG→AAG)	gabP	gamma‑aminobutyrate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/11);  total (16/11)

CCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAA  >  NC_000913_3_pae_tpiA/2795252‑2795533                                                                                                                                                 |                                                                                                                                          ccGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCGTCGCCGTTTCAAAGtt                                                                                                                                       >  1:242358/1‑149 (MQ=255)               gtTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCaaaa                                                                                                                         >  2:209525/1‑149 (MQ=255)                    tctcCACCGGCGCGGCATTCTTAACGTTGACCGGGAACCAATACGCGCCTGCGAAGGTACTGAAATTTCTGAACGACACCTCCGGAGCTATCGCCCCGCTGGTTCATCAAGACACCGCCGCGACAAAGGTGCGGACGCGCAAAAtacag                                                                                                                    >  2:404337/1‑145 (MQ=255)                               gcgGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCTGATGCGCAAAATTCTGCGAGCAgaagg                                                                                                         <  1:209525/149‑1 (MQ=255)                                                        aCTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTgg                                                                                <  2:46064/149‑1 (MQ=255)                                                        aCTATTAAACCCCTACGAAAGGATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCCGCGGGTTTATTTAGTCATCGCCGTGTCAAAGTGGCCGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAAttaga                                                                                <  2:180943/149‑5 (MQ=255)                                                          tATTACGCGCCTGCGAAGGTAGTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTAGCt                                                                              >  2:371243/1‑149 (MQ=255)                                                                  gcCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGt                                                                      >  2:60614/1‑149 (MQ=255)                                                                  gcCTGCGAAGGTATTTAAATTTCTGATCGACAGCTACGGCGCTATCGCACTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTATGCGAGCAGAAGGAAGCGACATTCGCTTGAGAATGTGGCTTTATCCGt                                                                      >  1:241081/1‑149 (MQ=255)                                                                      gCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCt                                                                  >  2:293607/1‑149 (MQ=255)                                                                                ttAAATGTCTGATCGACACCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCAACCAGAAGGAAGCTAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTa                                                        <  1:294831/149‑1 (MQ=255)                                                                                 tAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAg                                                       <  1:293607/149‑1 (MQ=255)                                                                                 tAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGCTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCTAATGTGGCTTTATCCGTGGCTCACCTGGCTAg                                                       <  1:197066/149‑1 (MQ=255)                                                                                         tGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTGGTCATTGGc                                               >  2:350157/1‑149 (MQ=255)                                                                                          gATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCt                                              >  2:33379/1‑149 (MQ=255)                                                                                           accgcccgCTGCGGCGTGATCGCCCTGATGGTTCATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt                                             <  1:159561/142‑1 (MQ=255)                                                                                               aCAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGGAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCGCTGGCTCACCTGGCTAGTCATCGGCTttatt                                         >  1:129555/1‑149 (MQ=255)                                                                                                     ccGGCGCTCTCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCttt                                   <  1:374202/149‑1 (MQ=255)                                                                                                        gcgcTATCGCCCTGCTGGTTTATTTAGTCATCGCCGATTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGCCTAGTCATCGGCTTTATTACCTTtgtg                                >  1:62625/1‑149 (MQ=255)                                                                                                             aTCGCCCTGCTGGTTTATGTAGTCATCGCCGTTTCAAGGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAAAGCGGCTTTAACGGTGGGTCACCTGGCTAGTCATCGGCTTTATTTCCTTTGTGTGGGt                           >  2:213747/1‑149 (MQ=255)                                                                                                                 cccTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGCAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTg                       >  1:165861/1‑149 (MQ=255)                                                                                                                     gctgGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAt                                                                                                <  2:233984/72‑1 (MQ=255)                                                                                                                     gctgGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAt                                                                                                >  1:233984/1‑72 (MQ=255)                                                                                                                        ggTTTTTTTAGTCCTCGGCGTTTGAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTAt                <  2:139614/149‑1 (MQ=255)                                                                                                                                 aGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCgg       >  1:184284/1‑149 (MQ=255)                                                                                                                                             ttCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCaa  <  2:311298/142‑1 (MQ=255)                                                                                                                                             ttCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCaa  >  1:311298/1‑142 (MQ=255)                                                                                                                                                 |                                                                                                                                          CCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAA  >  NC_000913_3_pae_tpiA/2795252‑2795533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑