New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 86746337 (1.010)4 (0.110) 4/268 4.6 10.3% coding (896/966 nt) iaaA Isoaspartyl peptidase
?NC_000913_3_pae_tpiA = 867471 34 (0.970)coding (904/966 nt) iaaA Isoaspartyl peptidase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGACCAGCAGAAAATAGCTGCG  >  NC_000913_3_pae_tpiA/867323‑867612
                                                                                                                                                    |                                                                                                                                             
cggATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACg                                                                                                                                               >  1:426558/1‑149 (MQ=255)
 ggATTAAGGCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCTCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGc                                                                                                                                              <  2:623283/149‑1 (MQ=255)
    ttAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATTGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAgg                                                                                                                                           <  1:555426/149‑1 (MQ=255)
          ctcGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATAc                                                                                                                                     <  1:60956/149‑1 (MQ=255)
           tcGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAAACGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCTTGGGGCTACCCAGGCGATAcc                                                                                                                                    >  2:157796/1‑148 (MQ=255)
                   gCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAaccac                                                                                                                            >  1:68906/1‑149 (MQ=255)
                     cTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCg                                                                                                                          >  1:230064/1‑149 (MQ=255)
                             gggTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTAc                                                                                                                  <  1:157779/149‑1 (MQ=255)
                                     aTGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGaaaa                                                                                                          <  2:403598/149‑1 (MQ=255)
                                        gAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAgg                                                                                                       >  2:260006/1‑149 (MQ=255)
                                                   tGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGc                                                                                           >  2:579042/1‑149 (MQ=255)
                                                     cgcTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCCCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGcc                                                                                          >  2:228512/1‑149 (MQ=255)
                                                        ttGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCcaca                                                                                       <  2:225452/149‑1 (MQ=255)
                                                                 gCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGtgatga                                                                              >  2:333743/1‑149 (MQ=255)
                                                                       gCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGa                                                                        <  1:333743/149‑1 (MQ=255)
                                                                        cTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACAGCGAAGGAATGTATCGCGCCGGGGGCGACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGAt                                                                       >  2:381952/1‑149 (MQ=255)
                                                                            aTCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCg                                                                   >  2:240899/1‑149 (MQ=255)
                                                                                cTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGGGCCTTGGGCTGCGCAGGCGATCCGCCAACCACCGGTATCTACCGTGAAAAATGGGACACCGTTTCCACACAGTGCTTAACTTGCTGCCGCTaa                                                               >  2:2164/1‑149 (MQ=255)
                                                                                 tATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACCCCGTTGGCACACCGTGATGAACTTGCTGCCGCTAAt                                                              >  1:35825/1‑149 (MQ=255)
                                                                                   tCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATg                                                                      <  1:600994/139‑1 (MQ=255)
                                                                                   tCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATg                                                                      >  2:600994/1‑139 (MQ=255)
                                                                                                     tCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCt                                          <  2:341021/149‑1 (MQ=255)
                                                                                                     tCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCAACGGTATGTCCGGTGAAGAAGGGGACACCGTTGCCACACAGAGATGAACTGAACGCCGGTAATGTGCTGGCGGTTgcgaacag                                          >  2:384608/1‑142 (MQ=255)
                                                                                                     gtgcgcTACCGTTTAACACCGAAGGAATGTACCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCt                                          <  2:206691/147‑1 (MQ=255)
                                                                                                               gTTTAACCCCGAAGGAATGAATCGCGCCGGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGcc                                <  2:483410/149‑1 (MQ=255)
                                                                                                                tttAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACGGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATCCCGGTAATGTGCTGGCGGTTGAAAATCGGAATATTGCCt                               >  2:313954/1‑149 (MQ=255)
                                                                                                                tttAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATGTGAATATTGCCt                               >  2:493597/1‑149 (MQ=255)
                                                                                                                   aaCACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATAc                                                                                                                                     <  1:62257/44‑1 (MQ=255)
                                                                                                                   aaCACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATAc                                                                                                                                     >  2:62257/1‑44 (MQ=255)
                                                                                                                      acCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAGATCTGAATATTGCCTTTATGc                         >  1:235382/1‑149 (MQ=255)
                                                                                                                        cGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAGAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAg                       <  1:197228/149‑1 (MQ=255)
                                                                                                                           aGGAATGTACCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGAc                    <  2:68906/149‑1 (MQ=255)
                                                                                                                            tgAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGAcc                   <  2:609609/148‑1 (MQ=255)
                                                                                                                                     cgcgCCTGGGGCAACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGACCAGCAGaaaa          <  1:228512/149‑1 (MQ=255)
                                                                                                                                          cTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGACCAGCAGaaaa          <  2:25647/144‑1 (MQ=255)
                                                                                                                                          cTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGACCAGCAGaaaa          >  1:25647/1‑144 (MQ=255)
                                                                                                                                             gggCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGACCAGCAGAAAATAGCTGCg  <  2:230064/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
CGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACACAGTGATGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTGAATATTGCCTTTATGCAGGACCAGCAGAAAATAGCTGCG  >  NC_000913_3_pae_tpiA/867323‑867612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.