Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 482,230 G→A P725L (CCG→CTG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA482,2300GA96.9% 87.7 / NA 33P725L (CCG→CTG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (19/12);  minor base C (1/0);  total (21/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGC  >  NC_000913_3_pae_tpiA/482089‑482375
                                                                                                                                             |                                                                                                                                                  
tAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGACTGCGCTTTTTACTGGCCAATATCAGTACTGAATTGCAGGGTATc                                                                                                                                            >  2:108835/1‑149 (MQ=255)
           cacaCGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTGCAGGTCGATGTCAGTCTTAAACTGCAGGGTATCTTCCAGACCGt                                                                                                                                 >  1:482171/1‑149 (MQ=255)
            acacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGtt                                                                                                                                >  2:211985/1‑149 (MQ=255)
             cacGGCCGCGGTCGATAAAAGCGTTCACATAGCTGGCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGTTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGttt                                                                                                                               <  2:581046/149‑1 (MQ=255)
              acGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTg                                                                                                                              <  1:26666/149‑1 (MQ=255)
                gACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGa                                                                                                                            <  1:251052/149‑1 (MQ=255)
                          gATAAAGTCGTTCACATAGCTGCCGCACCATGCAGCGCCCAGAGTGGTGTTAAGGTCGTTGATAGGAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTCAACTGCCGGGTATCTCCCAGACCGTGTGCACGTCCGCTgg                                                                                                                  >  2:174757/1‑149 (MQ=255)
                            tAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGACTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATGTCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGCTc                                                                                                                >  2:588682/1‑149 (MQ=255)
                              aaGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCaa                                                                                                              <  1:211985/149‑1 (MQ=255)
                                    ttCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATc                                                                                                        >  1:540573/1‑149 (MQ=255)
                                       acaTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACGGTTTGGACGTACGCTGGTCAACatagaagg                                                                                                     >  1:254846/1‑144 (MQ=255)
                                         aTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGATGTACGCTGGTCAACACATCAgtgt                                                                                                   >  2:239339/1‑149 (MQ=255)
                                         aTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGt                                                                                                   >  1:331050/1‑149 (MQ=255)
                                               gccgccCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCg                                                                                             <  1:502516/149‑1 (MQ=255)
                                                ccgccCCATGCAGCGCCCAGAGTGGCGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgc                                                                                            <  1:333326/149‑1 (MQ=255)
                                                   ccccATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTTGTCTTTATCAATCTTAAACTGCAGGCTATCTTCCAGACCGTTTCGACGTACGCTGCTCAACATATCACCGTCCTTCcctcc                                                                                         >  2:369485/1‑147 (MQ=255)
                                                     acATGCAGCCGCCAGGGTTGTGTTAATGTCGGTGATAGAAACACCCAGCGCCTGCGCTGTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGCACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCtt                                                                                       <  2:404506/148‑1 (MQ=255)
                                                           aGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaa                                                                                 <  1:445230/149‑1 (MQ=255)
                                                                          gtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTAcgcg                                                                  >  1:48181/1‑149 (MQ=255)
                                                                          gtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGCTTGGACGTACGCTGGTCGACATATCAGGGTGCTTCGCTGCTTCGGCAAGCAACTGGTTAcgcg                                                                  >  1:339432/1‑149 (MQ=255)
                                                                            tAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGcc                                                                >  1:14073/1‑149 (MQ=255)
                                                                             aaTGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTAAGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCt                                                               <  2:367532/149‑1 (MQ=255)
                                                                              aTGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCACCTGGTTACGCGCCTg                                                              >  2:571663/1‑149 (MQ=255)
                                                                               tGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGAGCCTGa                                                             >  1:163637/1‑149 (MQ=255)
                                                                                       aTAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                                     >  1:602341/1‑149 (MQ=255)
                                                                                                        cTGCGCTTTTTCCTGGTCGATATCACAACAACTTGAGAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAg                                    >  2:598780/1‑149 (MQ=255)
                                                                                                                     tGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg                       >  1:189548/1‑149 (MQ=255)
                                                                                                                           atatCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa                 <  2:163637/149‑1 (MQ=255)
                                                                                                                                  tCTTAAACTGCAGGGTATCTTCCGGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCAAGCCTGGTCAATCAGCtcaaagtcaaag          >  2:317792/1‑149 (MQ=255)
                                                                                                                                  tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAGGGCCAGCCTGGGCAATCAGCtcaaagtcaaag          >  1:148983/1‑149 (MQ=255)
                                                                                                                                  tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag          <  2:167391/149‑1 (MQ=255)
                                                                                                                                        aCTGCAGGGAATCTTCCAGACAGATTGGACGTACGCTGGCAAACAAACCACGATGCTCCACCAGCTGGACGTGCAGCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCACGCTCGCCAACCAGCCCAAAGTCAAACCCgctc    >  2:132116/1‑146 (MQ=255)
                                                                                                                                          tGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGc  <  1:409242/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
TAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGC  >  NC_000913_3_pae_tpiA/482089‑482375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: