Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,483,617 Δ5 bp coding (557‑561/3903 nt) hrpA → putative ATP‑dependent helicase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1483617 1483621 5 24 [0] [0] 24 hrpA putative ATP‑dependent helicase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 14836160 (0.000)24 (0.660) 22/282 0.8 100% coding (556/3903 nt) hrpA putative ATP‑dependent helicase
?NC_000913_3_pae_tpiA 1483622 = 0 (0.000)coding (562/3903 nt) hrpA putative ATP‑dependent helicase

TGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/1483473‑1483616
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGA  >  NC_000913_3_pae_tpiA/1483622‑1483758
                                                                                                                                                                                                                                                                                         
TGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGAT                                                                                                                                      >  1:597666/1‑149
    GAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCAG                                                                                                                                  <  2:549881/149‑1
       CCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCAGTAC                                                                                                                               >  1:405493/1‑149
                      GCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGCTCACGAAAGTGCACAAACGATGTACAACCTGATAACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATT                                                                                                                <  2:547329/149‑1
                        gcaagACGGAGCCGGGCGGTTACATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGA                                                                                                              <  2:30607/144‑1
                        TGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGA                                                                                                              >  1:391995/1‑149
                        TGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGA                                                                                                              >  1:616701/1‑149
                        TGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGA                                                                                                              <  2:326413/149‑1
                                            TGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCC                                                                                          <  2:405493/149‑1
                                               ATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGA                                                                                       >  1:101709/1‑149
                                                TCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGACGAAGGGCACGAACGCAGCCTGAA                                                                                      >  1:295357/1‑149
                                                    TTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATC                                                                                  <  1:202788/149‑1
                                                                               AAGTGATAACCTGGTGGTCATGCTGATCACCGACGGTATCCTGCAGGCGGAGATCCAGCAAGACCCTGATGCAGTACTACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAG                                                       <  1:362663/149‑1
                                                                                  TGAGAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTG                                                    <  1:603117/149‑1
                                                                                         ACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGC                                             >  2:89199/1‑149
                                                                                          CGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGGCCCTGATGCAGTACGAGGCTATGATTATTGACGAAGCGCACGAACGCGGCCTGAATATCGATTTTTTGCTCGGCTTTTTGGAGGAGTTGCTGCCGag                                            >  2:149427/1‑147
                                                                                               GTCAAGAGGATGACGGACGGTATCCTGCTGCCGGAGATCCAGCAAGCCCCTGATGCAGTACGACACTATCATTATTGACGAAGCGCAGGAACGCAGCTTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTC                                       <  2:493170/149‑1
                                                                                                TCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCC                                      >  1:387724/1‑149
                                                                                                        ATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCCTGATGCCGTACGACACTATCATTATTGACGAAGGGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAA                              >  1:256743/1‑149
                                                                                                                 GGTATCCTGCTGGCGGAGATCCAGCAAGACCGTGATGCAGTAGGACACTATGATTATTGACGAGGCGCACGAACGCAGCCTGGATATCGATTTTTTGCTAGGCTTTTTGAAAGAGTTGGTGCAGCGGCGTTCTGACCTTAAAATCATTA                     >  2:28487/1‑149
                                                                                                                           TGGCGGAGATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGC           >  1:398094/1‑149
                                                                                                                               GGAGATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACT       >  2:474631/1‑149
                                                                                                                                  GATCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATC    <  2:391995/149‑1
                                                                                                                                    TCCAGCAAGACCCTGATGCAGTACGACACTATCATTATTGTCGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGA  <  2:231893/149‑1
                                                                                                                                                                                                                                                                                         
TGGCGAACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/1483473‑1483616
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGA  >  NC_000913_3_pae_tpiA/1483622‑1483758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.