| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 264,098 | G→T | 100% | L50L (CTC→CTA) | ykfH ← | uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 264,098 | 0 | G | T | 100.0% | 92.2 / ‑6.7 | 38 | L50L (CTC→CTA) | ykfH | uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (24/12); total (25/13) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAATTT > NC_000913_3_pae_tpiA/263961‑264243 | aGGCGCAGGGTAATCTCCCGCTGCAGGCCCCTGGTAGGATAGCTCATTACAGTTCTCCACTATTGTCAGGTCAGGGGGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCGGGc > 1:514901/1‑149 (MQ=255) cAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatat > 1:177974/1‑149 (MQ=255) aTCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTATACAATGCTGTCAGTTCAGGGTGATGCTATTCAGGCAGGTATAGGGCCCATTGCGGTCCTGGGGGCATTCGGCGTATACCGCTAGTAGTCACGCGATATCCGGaat > 2:482619/1‑148 (MQ=255) tctcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTGCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACg > 1:86025/1‑149 (MQ=255) tctcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCACATCAGGCAGGTATAGGGCCCATTGCGGTCCTCGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCAATATCCGGAACg > 1:308998/1‑149 (MQ=255) cccGTTGCCGGCCCCTGCCATGGCTGGTCATGAGTGTGTTCGACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTcc < 1:705058/149‑1 (MQ=255) cccGCTCGAGGCTCCTGGTAGGGTTGCCAATTACAGTTCTCCTCTATTGGCAGTTTAGGGTGGTGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTcc < 2:380763/149‑1 (MQ=255) ccGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTCTTGTCAGTTCAGGGTTATGCTCATCAGGCAGGTCTAGGGCCCATTGCGGTCCTGGCGGCGTTCGCCGTATACCGCTCAGACTCCGGCGGGACCCGGAACGTccc > 2:604802/1‑149 (MQ=255) cccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCGGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTaa > 1:411731/1‑149 (MQ=255) cccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTaa < 1:641846/149‑1 (MQ=255) aTTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACGGACGCTACCGTg > 1:190474/1‑149 (MQ=255) tACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGACCACCACAGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGcc < 1:722963/149‑1 (MQ=21) ttCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCGTTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTgg > 1:91822/1‑149 (MQ=255) ttCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGCGTAATGACAGACGCTACCGTCCCACTgg > 2:542394/1‑149 (MQ=255) tcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAt > 1:612358/1‑149 (MQ=255) tcCACTATTGTCAGTTCAAGGTGATGCTCATCAGGCAGGTAACGCGCCCGTTGAGGTCCTGGCGGCGTTCGGCGTATACCCCTAGGACTCCGGCGATAACCGGACCGTCCCTGCCGGTGTGCTCACCGCCGCTACCGCCCCATTGGTAt > 2:304288/1‑149 (MQ=255) cACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTAGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAttt > 1:367856/1‑149 (MQ=255) tgtgtgAGTGCAGGGTGAGGCTAGTCGGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCg < 2:647554/147‑1 (MQ=21) tGTCAGTTCAGGGTGATGCTCATCAGGCAGGTCTTGCATGCATGTCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGCACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGt > 1:356312/1‑149 (MQ=21) tCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGc > 1:49554/1‑149 (MQ=255) tCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGACAGTGc > 2:196193/1‑149 (MQ=255) gggTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGCCGATATTCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGa < 1:468368/149‑1 (MQ=255) cTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACGCCGGCGATATCCGGAACGTCCCTGCCGGGGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCg > 1:69908/1‑149 (MQ=255) tataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGc > 1:661873/1‑149 (MQ=255) tcgggCCCTTTGCGGTCCTGGCGGTGTTGGGCGCATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTg < 1:160309/147‑1 (MQ=17) ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCg > 1:733352/1‑149 (MQ=255) ggCCCATTGCGGTCCTGGCGCCGTTCGGAGTATCCCGGGAGGACTCAGGCGATATCCGGAACGTACCGGCCGGTGTAGTGACAGACGCTAGCGTGACACAGGCATTGGCCGGCGCAGTAGCGAAAGATTCGGGGCGCAGGGTGCTGGca > 2:584594/1‑148 (MQ=255) tGCGGTCCTGGCGGCGTTCGGCGTAGACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCAGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATc < 1:663343/149‑1 (MQ=255) tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCAt > 1:432828/1‑149 (MQ=255) tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCAt > 1:447237/1‑149 (MQ=255) cggggTTCGGCGTATACCGCTAGGCCGCCGGCGATATCCGTAACGCCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATCCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCt < 2:571999/149‑1 (MQ=17) cggcggATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAACGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTa < 1:29138/149‑1 (MQ=255) ggCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTAc > 1:475392/1‑149 (MQ=255) gggTACACCGCTAGGTCTCCGGTGAGCTCCGGAACGGCCCTGCCGGTGTAATGACATACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACt < 2:656072/147‑1 (MQ=14) gTATGCCGCGGGGACGCCGGCGCTATCCGGAACGTCCCTGCCGGTGTAAGGACAGACGCTACAGTGCAGCTGGTACTTCCCGGCCTGGTGTTGAAAGATGCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGa < 1:353036/149‑1 (MQ=14) tcccGCTAGGACTGCGGCGAAATCCGGAACGTCCCGCCCGGTGTAATGACAGAGGCTACCGTGCCTCTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATaa < 1:161779/147‑1 (MQ=14) aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCACGATGCAGGGCGCCTATCCTCATTGCCCAGCGTCTACTGATAAt > 2:285150/1‑149 (MQ=14) gctagaggagTCGGGGGTTATGCGGGACGTTCCTGCCGGTGTAATGACAGACGCTACCGTGCCGATGGTATTTGACGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAAtt < 2:104845/145‑1 (MQ=14) ccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCAGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGCTAAtc > 2:559175/1‑148 (MQ=255) cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGACAGATTCGGGACTGAGGATACTCGCGGTATACCGTTACCGCCCTGCGGTGCCTGATAGttt > 2:299629/1‑149 (MQ=17) cgcaAGCACTGCGGCGAGATCCGTAACGTCCCTGCCGGTGCAATGACACACGCTCCCGTGCCACTGGTATTTTCCCGTGCAGTAGCGAAAGATTCGTGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAAttt < 1:406570/149‑1 (MQ=14) | AGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAATTT > NC_000913_3_pae_tpiA/263961‑264243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |