| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 275,065 | G→A | 100% | T18I (ACC→ATC) | insH1 ← | IS5 transposase and trans‑activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 275,065 | 0 | G | A | 98.3% | 48.1 / ‑6.7 | 22 | T18I (ACC→ATC) | insH1 | IS5 transposase and trans‑activator |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (9/10); minor base C (0/1); total (9/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTAT > NC_000913_3_pae_tpiA/275003‑275207 | gggACACGAAAATCTCTTTTCTGGTCTAACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGACTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCgg > 2:380974/1‑149 (MQ=9) aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCCCCTTCCGGAGGCg > 1:472006/1‑149 (MQ=25) aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCg > 1:179685/1‑149 (MQ=25) aaaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTAt < 1:234715/149‑1 (MQ=38) aaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATg > 1:671434/1‑149 (MQ=255) tctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAg < 2:158974/149‑1 (MQ=255) tctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACAAGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAg < 1:663660/149‑1 (MQ=255) ttttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGCGGCGTTATGAGCTgg > 1:416165/1‑149 (MQ=255) ttttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTTTGAGCTgg > 1:130252/1‑149 (MQ=255) ttgcTGGGCTCACGGTGCTTACTGCTGATGTCACGCTGGGAGAATCTAAGTTGATGACTCATGATGAACTCTGTTTCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTggc < 2:89000/146‑1 (MQ=14) tgcggTCGGACGGCGCTTACGGCTGAATTCAGTGTCGTCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGAAAAACATGATCCCATATCAGGGACTTCTTCGCAACTTCCGGCGGCATTATGAGCTggcgg < 2:96656/146‑1 (MQ=14) tGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGcc > 1:176188/1‑149 (MQ=255) tGATGGCGCTTACTGCTGAATTCACTGTCGGCGAAGAGTAGTTGAGGACTCATGCTGAACCCTGTTCTATGGCTCCGGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCtttt < 2:620320/149‑1 (MQ=17) gACGGAGCTTATGGCTCAATTCACCTTCGGCGAAGGTAAGTTGATGACGCATGAGGAACCCTGTTCTATGGGTCGAGATGCCAGACATGATCTCATATCAGGGACGTGTTCGCTCCTTCCGGAGGCGTTATGAGCTGGCGGCCCttttt < 2:196739/149‑1 (MQ=17) gctcgtACTGCTGAATTCATTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTCTGGCTCCAGATGACACACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTGTTTTAt < 2:88748/144‑1 (MQ=17) gcgcTGACTGCGGAATTCACTGTCGGCGAAGTGATGTTTATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACTAACATGATCTCATATCAGGGACTTTTTCGGACCTTCCGGAGGCGTTATGAGCTGGGGGCCCTTTTTGTAt < 2:278899/149‑1 (MQ=17) tggtgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGTTGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGTGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGattat < 2:5131/146‑1 (MQ=255) tgctgAATTCACTGTCGGCGAAGAGAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGTGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGattat < 2:123320/149‑1 (MQ=255) aaTTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATc < 1:721081/149‑1 (MQ=255) aaTTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGACCCTTTTTGTATCTGATTATTAATc < 1:358557/149‑1 (MQ=255) tGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATGATTAATCCCCAccc > 2:273962/1‑149 (MQ=255) gCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTTATCCCCACCCGCTac > 2:133460/1‑148 (MQ=255) | GGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTAT > NC_000913_3_pae_tpiA/275003‑275207 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |