Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1978503–1979270	1979278	9–776	35 [0]	[0] 35	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1978502	0 (0.000)	32 (0.730)	28/266	0.7	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_pae_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

ATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAAT  >  NC_000913_3_pae_tpiA/1979135‑1979425                                                                                                                                                 |                                                                                                                                                   atgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATaa                                                                                                                                                <  2:291156‑M2/5‑1 (MQ=255)     gtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagaacacacaaaacactcaattacttaacataaatgTATAagtaa                                                                                                                                            >  2:480206‑M2/141‑147 (MQ=255)        tttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATAc                                                                                                                                         >  1:818195‑M2/138‑149 (MQ=255)               ttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaccataaatgTATAAGTCATActtttgtg                                                                                                                                  >  2:381100‑M2/131‑148 (MQ=255)               ttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgtt                                                                                                                                  >  2:611731‑M2/131‑149 (MQ=255)                   ctaatatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                              <  1:630180‑M2/23‑1 (MQ=255)                   ctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacacttcatgatgtaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                              <  2:346543‑M2/23‑1 (MQ=255)                    taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacataaaacggaggggggctgcgattttcaataatgcgtgatgcagaccacacaaaaaaagaaattacttaacataaatgGATAAGTCATACATTTGTTGTggg                                                                                                                             >  1:558090‑M2/126‑149 (MQ=255)                        atttttcttaaattgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgctgcagatcacacaaaacactcaataacttaacataaatgTATAAGCTACACTCTTGTTTTGTGTGTa                                                                                                                         >  2:547656‑M2/122‑149 (MQ=255)                            ttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTc                                                                                                                     >  1:412069‑M2/118‑149 (MQ=255)                                   atcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGt                                                                                                              <  2:730439‑M2/39‑1 (MQ=255)                                    tcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgctgatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGtt                                                                                                             <  1:795659‑M2/40‑1 (MQ=255)                                         gcaactgtactcgtcactacccgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaaaaa                                                                                                        <  1:790717‑M2/45‑1 (MQ=255)                                              tgtactcgtcactacacgcacatacaaaggtgtagacctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGtttt                                                                                                   >  1:608416‑M2/100‑149 (MQ=255)                                                tactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTc                                                                                                 >  2:534275‑M2/98‑149 (MQ=255)                                                tactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTc                                                                                                 <  2:279716‑M2/52‑1 (MQ=255)                                                  ctcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTAAAAAAGTTTTTcgc                                                                                               >  1:695988‑M2/96‑149 (MQ=255)                                                                    tacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaaa                                                                             <  1:131503‑M2/72‑1 (MQ=255)                                                                            aggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAg                                                                     <  1:534275‑M2/80‑1 (MQ=255)                                                                                    tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATc                                                             <  2:120602‑M2/88‑1 (MQ=255)                                                                                           ttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATgg                                                      <  1:611731‑M2/95‑1 (MQ=255)                                                                                              aataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGAt                                                   <  1:687701‑M2/98‑1 (MQ=255)                                                                                              aaaaatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGATACGCTAGCAAGCAAAAATGAAACAGAAGTAATCGAAATGGGAt                                                   >  1:241835‑M2/52‑149 (MQ=255)                                                                                                  atgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTg                                               <  1:4434‑M2/102‑1 (MQ=255)                                                                                                   tgcgttatgcagataacacaaaactataaattccttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCCGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAGTTAGatgtatc                                              <  2:347131‑M2/103‑4 (MQ=255)                                                                                                      gtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAACCAAAAATCAAACAGGAATAATCGAAATAGAATGTTGcaca                                           >  2:453410‑M2/44‑149 (MQ=255)                                                                                                        gatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGcaca                                         <  1:509680‑M2/108‑1 (MQ=255)                                                                                                            cagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCa                                     >  1:32471‑M2/38‑149 (MQ=255)                                                                                                               atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCaaaa                                  <  2:759058‑M2/115‑1 (MQ=255)                                                                                                                tcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTTCCCACAGtcaaatt                                 >  2:389087‑M2/34‑147 (MQ=255)                                                                                                                             tcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTaaa                    <  2:695988‑M2/129‑1 (MQ=255)                                                                                                                                    cttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGGTGTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAatcat             >  1:27876‑M2/14‑149 (MQ=255)                                                                                                                                          ataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCta       >  2:87021‑M2/8‑149 (MQ=255)                                                                                                                                             aatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAACTTAATAGCGACGTTGGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCCTCTGCTataa    >  1:568860‑M2/5‑149 (MQ=255)                                                                                                                                               tgTATAAGTCATACTTTTGTTTTGGATGTATTTAAATCTGTTAAAAATTTTTTCGCTACGCTAACAAGCAAAAATGAAACAGAAATAATCTAAATGGGATGTTGCTCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTataaat  <  2:558090‑M2/147‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   ATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAAT  >  NC_000913_3_pae_tpiA/1979135‑1979425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑