Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	2,679,525	G→T	A1074D (GCT→GAT)	yphG ←	DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	2,679,525	0	G	T	100.0%	104.2 / NA	35	A1074D (GCT→GAT)	yphG	DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/18);  total (17/18)

AGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTT  >  NC_000913_3_pae_tpiA/2679380‑2679669                                                                                                                                                  |                                                                                                                                                 aGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAATTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCg                                                                                                                                               >  1:197197/1‑149 (MQ=255)    gTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCTGCTTTTGGCCAATCCGGAt                                                                                                                                            >  1:443916/1‑149 (MQ=255)         tattaATTACGGTTTATTCTAATTATTTTGTGATGGGCGGCGGCGCGTGCCGCTCAAATATTGCAGTTAGTAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGTATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATc                                                                                                                                       <  1:530958/149‑1 (MQ=255)         tattaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGTATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATc                                                                                                                                       <  1:555813/149‑1 (MQ=255)                     cTTATTCTAATTATTTTGTGATGGGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCa                                                                                                                           <  2:32283/149‑1 (MQ=255)                      gtATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTAGGCCAATCCGGATCAATCGACCTCAATTCAg                                                                                                                          >  2:473717/2‑149 (MQ=255)                            tAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCacggccaa                                                                                                                    >  1:301922/1‑142 (MQ=255)                               ttattTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAg                                                                                                                 >  1:491896/1‑149 (MQ=255)                                           gaGCGGCAGGTCGTGCCTTTCAAATATTACAGTTAATAGGGTTTAGTGAATGCTGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgcg                                                                                                     <  2:41879/149‑1 (MQ=255)                                                    cgcgTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTccc                                                                                            >  1:515733/1‑149 (MQ=255)                                                      cgTGCCGCTCAAATATTACAGGTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCg                                                                                          <  2:491896/149‑1 (MQ=255)                                                           cGCTCAAATATTACAGTTAATAGGGTTTAGTTAACGCGGTTAAAAATAAAATGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGTATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTcc                                                                                     <  1:361752/149‑1 (MQ=255)                                                                  atatTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACTTCCCCGAGTCCCGCGGAc                                                                              <  2:566442/149‑1 (MQ=255)                                                                   tatTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACg                                                                             >  1:665057/1‑149 (MQ=255)                                                                     ttACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGcc                                                                           <  1:612261/149‑1 (MQ=255)                                                                         aGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCCGCa                                                                       >  1:152294/1‑149 (MQ=255)                                                                         aGTTAATAGGGTTCAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCa                                                                       >  2:254826/1‑149 (MQ=255)                                                                                    tttAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCACCATCGCCCCCATc                                                            >  1:324376/1‑149 (MQ=255)                                                                                          gAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAt                                                      <  1:254826/149‑1 (MQ=255)                                                                                                ggTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAgg                                                         <  1:357927/140‑1 (MQ=255)                                                                                                ggTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAgg                                                         >  2:357927/1‑140 (MQ=255)                                                                                                 tcctataaTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGACAGCATAGCCCCCATCAGGCATTTTTCtt                                               >  2:611258/7‑149 (MQ=255)                                                                                                  ttAAAAATAAAAGGCATCACGTTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGACAATTCAGCGTGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCttt                                              <  2:77213/149‑1 (MQ=255)                                                                                                   tAAAAATAAAAGGCAGCACGTTGTTGAATAATGCCGCTTTCTGTCAATCCGTATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCGCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTa                                             <  2:313051/149‑1 (MQ=255)                                                                                                    aaaaaTAGAAGGCATTACGGTGGGGAAGAATGCCGCTGTCGGCCAATCTGGATTAATCGCAGTCAATTCATCGCGAGCAGTCTCATATTGCGCAACCTCCCCGCTTCCCGCTGACGCCAGCATCGCTACCATCAGGCATTTTTCTTTAt                                            <  2:91416/149‑1 (MQ=255)                                                                                                    aaaaaTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAt                                            <  1:536333/149‑1 (MQ=255)                                                                                                    aaaaaTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCCGACCCCCACAACGCCACCATCAGGCATTTTTCTTTAt                                            <  2:152294/149‑1 (MQ=255)                                                                                                           aaaGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGctgctg                                     >  2:262601/1‑149 (MQ=255)                                                                                                                     cGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCg                           >  2:469208/1‑149 (MQ=255)                                                                                                                           tgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATAc                     >  1:617541/1‑149 (MQ=255)                                                                                                                              aaaatGCCGCTTTCTGCCAATCCGGATTAATCGCCTTCAATTCATCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACTCCAGCATCGTCGCCAGCAGGCATTTTTCTTTATGCTGCTGTTGCAATTCGTCAGACAGc                  <  2:84889/147‑1 (MQ=255)                                                                                                                                aaggCCGCTGTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGAGAGCAGCCTCATTAAGACCCTACACCCCCCGTCCCGCTTGCGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGa                <  1:139254/146‑1 (MQ=255)                                                                                                                                   gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGacaa             <  1:427929/149‑1 (MQ=255)                                                                                                                                             gCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGt   >  1:640249/1‑149 (MQ=255)                                                                                                                                              ccAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTGGCTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGtt  >  1:481849/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                 AGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTT  >  NC_000913_3_pae_tpiA/2679380‑2679669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑