Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,011,2780TG22.1% 114.9 / 30.6 69L68R (CTT→CGT) yigMputative inner membrane EamA‑like transporter
Reads supporting (aligned to +/- strand):  ref base T (30/23);  new base G (8/7);  total (39/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.28e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GGCGAGTACCTTGCGGGGCACGTCGATAGCTATTTTGCGGTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGCTGATTTATGACATCATGAGTAAGCGCCGTCTGCGCTGGGGCTATGCCT  >  NC_000913_3_pae_tpiA/4011130‑4011421
                                                                                                                                                    |                                                                                                                                               
ggCGAGTACTTTGCGGGGCACGTCGATAGCTATTTTGCGGTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCt                                                                                                                                                 <  2:735136/149‑1 (MQ=255)
     gTACCTTGCGGGGCACGTCGATAGCTATTTTGCGGTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCGTGGCg                                                                                                                                            >  1:671209/1‑149 (MQ=255)
      tACCTTGCGGGGCACGTCGATAGCTATTTTGCGGTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCGTGGCGt                                                                                                                                           <  2:55462/149‑1 (MQ=255)
         cTTGCGGGGCACGTCGATAGCTATTTTGCGGTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGAt                                                                                                                                        <  2:443045/149‑1 (MQ=255)
            gCGGGGCACGTCGATAGCTATTTTGCGGTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGta                                                                                                                                     <  1:372439/149‑1 (MQ=255)
             cGGGGCACGTCGATAGCTATTTTGCGGTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGtat                                                                                                                                    <  1:331207/149‑1 (MQ=255)
              ggggCACGTCGATAGCTATTTTGCGGTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGtata                                                                                                                                   >  1:813622/1‑149 (MQ=255)
              ggggCACGTCGATAGCTATTTTGCGGTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTACTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGtatc                                                                                                                                   <  2:746078/149‑2 (MQ=255)
                ggCACGTCGATAGCTATTTTGCGGTGCTGGTGCGCGTGGGCCTGGAGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATg                                                                                                                                 <  1:720024/149‑1 (MQ=255)
                    cGTCGATAGCTATTTTGCGGTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCGTGGCGTGATGTATATGCTGa                                                                                                                             <  2:245066/149‑1 (MQ=255)
                      gcGATAGCTATGTTGCGGTGCTGGTGCGGGTTGGACTGGCGGCACTCGTTTTTCTGCGGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCTTGTATATGCTGGTGGGCGCGATGCAGCGTGGCGTGATGTATATGCTGAGt                                                                                                                           <  2:922046/148‑1 (MQ=255)
                        gATAGCTATTTTGCGGTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGttt                                                                                                                         >  2:421696/1‑149 (MQ=255)
                            gCTATTTTGCGGGGCTGGTGCGCGTTGGCCTGGCGGCCCTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCcgc                                                                                                                     <  1:904916/149‑1 (MQ=255)
                              tATTTTGCGGTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCGTGGCGTGATGTATATGCTGAGCTTCCgcgc                                                                                                                   >  1:129530/1‑149 (MQ=255)
                                ttttGCGGTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCGTGGCGTGATGTATATGCTGAGTTTCCGCGCtt                                                                                                                 >  2:723041/1‑149 (MQ=255)
                                       gTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGTGCGCGATGCAGCGTGGCGGGATGTATATGCTGAGTTTCCGCGCTTATCTCTa                                                                                                          >  1:181155/1‑149 (MQ=255)
                                        tGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGa                                                                                                                                         <  2:99887/117‑1 (MQ=255)
                                        tGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGa                                                                                                                                         >  1:99887/1‑117 (MQ=255)
                                             gTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGAc                                                                                                    >  1:383545/1‑149 (MQ=255)
                                                  cgTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGttt                                                                                               >  1:794534/1‑149 (MQ=255)
                                                       gCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAg                                                                                          <  1:556916/149‑1 (MQ=255)
                                                         cTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCGTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAgct                                                                                        >  1:154735/1‑149 (MQ=255)
                                                         cTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGGCGGCCTGTATGTGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTGATCTCTCCCTGACCGTATCCGAgct                                                                                        >  2:723202/1‑149 (MQ=255)
                                                         cTGGCGGCACTCGTTTTTCTGCCGATTCTGCGTACCCGTGGCAATAGCCTGACAACGCTCGGCCTGTATATGATGGGGGGCGCGATGCAGCCTAACAAGTAACATAGGCGGAGAATCCCAGAAAATGTCTACCTGACGGTTTCCGAgct                                                                                        >  1:961453/1‑149 (MQ=255)
                                                                 aCTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGt                                                                                >  2:377576/1‑149 (MQ=255)
                                                                 aCTCGTCTTTCTGCCGGTTCGGCGTACCCGTGGCAATAGGCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGGTGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGt                                                                                <  2:38310/149‑1 (MQ=255)
                                                                  cTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTGTCCGTTTCGTTGCCTCGCTTCTCTCTCCCTCACGGTTTCCGAGCTGCTGCTGtt                                                                               >  1:224784/1‑149 (MQ=255)
                                                                  cTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGtt                                                                               >  1:194088/1‑149 (MQ=255)
                                                                  cTCGTTTTTCTACCGTTGTTGCGTACTCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCGTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGtt                                                                               >  2:585589/1‑149 (MQ=255)
                                                                      ttttttTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCAcc                                                                           <  1:27732/149‑1 (MQ=255)
                                                                          tCTGCCGTTTCTGCGTACCCGTGGTAATAGCCTGAAAACGGGCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGTGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGc                                                                       <  1:212405/149‑1 (MQ=255)
                                                                          tCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCGTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGc                                                                       <  2:19253/149‑1 (MQ=255)
                                                                           cTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCGTGGCGTGATGTATATGTCCAGGGGTCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCt                                                                      >  1:722870/1‑149 (MQ=255)
                                                                            tGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGCGGGCGCGATGCAGCTTGGCGTGATATATATGCTGAGTTTCCGCCCTTATCTCTACCTGCCGGTTTCCGAGCTGCTGCTGTTCAcgctcctg                                                                     >  2:85073/1‑142 (MQ=255)
                                                                             gCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTAcc                                                                                                        <  1:199511/113‑1 (MQ=255)
                                                                             gCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTAcc                                                                                                        >  2:199511/1‑113 (MQ=255)
                                                                              ccGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTcc                                                                                                                       >  2:99700/1‑97 (MQ=255)
                                                                              ccGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTcc                                                                                                                       <  1:99700/97‑1 (MQ=255)
                                                                              ccGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCACTGCGGCTGGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGGTCACCGCGCTgcc                                                                   >  2:40804/1‑147 (MQ=255)
                                                                                gTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGAcgc                                                                 >  1:106562/1‑149 (MQ=255)
                                                                                gTTGCTGGGGACCCGTGGCAATTGCCTGAAAACGGTTGGCCTGTATATGCTGGTGGGCGGGATGCAGCTTGGCGTGATGTATATGCTGAGTTTTCGTGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGAcgc                                                                 <  2:575322/149‑1 (MQ=255)
                                                                                 tttCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGAcgcc                                                                >  1:537681/1‑149 (MQ=255)
                                                                                     tGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGa                                                                                                     <  1:498948/108‑1 (MQ=255)
                                                                                     tGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGa                                                                                                     >  2:498948/1‑108 (MQ=255)
                                                                                       cGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTa                                                          <  1:423653/149‑1 (MQ=255)
                                                                                        gTACGCGTGGCAATAGCCTGAAAACGGTGGGCTTGTATAGGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTAc                                                         <  2:270324/149‑1 (MQ=255)
                                                                                         tACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGAAGGTTTCCGAGCTGCTGCGGTTCACCGGGCTGACGCCGCTCTACa                                                        >  1:604896/1‑149 (MQ=255)
                                                                                            ccGTTGCAATAGCCTGAAAACGGTCGGCCTGTAGATGCTGGTGGGCGTGATGCAGGTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCa                                                     <  2:547667/149‑1 (MQ=255)
                                                                                            ccGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATcc                                                     >  1:355970/1‑148 (MQ=255)
                                                                                            ccGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCa                                                     >  1:357003/1‑149 (MQ=255)
                                                                                              gTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATATATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTTCTGACGCCGGTCTACATCACg                                                   >  2:62692/1‑149 (MQ=255)
                                                                                               tGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTTACGCCGCTCTGCATCCCGc                                                  >  1:932/1‑149 (MQ=255)
                                                                                                   aaTAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGCTGAt                                              >  2:519332/1‑149 (MQ=255)
                                                                                                         cTGAAAACGGTCGGCCTGTATATGCTGGTGTGCGCGATGCAGCGTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGCTGATTTATGa                                        <  2:181155/149‑1 (MQ=255)
                                                                                                         cTGAAAACGGTCGGCCTGTATATGCGGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGCTGATTTATGa                                        <  2:273596/149‑1 (MQ=255)
                                                                                                          tGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGGGTTTGCGCGCTTCTCTCTACCTGCCGGTTTCCGAACTGCTGATGTATCCCGTGGTGCCGCCGGTCTCCCTCACCCTTATTTATGat                                       >  2:17056/1‑148 (MQ=255)
                                                                                                             aaaCGGGCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGCTGATTTATGAcatc                                    <  1:901827/149‑1 (MQ=255)
                                                                                                               aCGTTCGGCCTGTTTATGCTGGTGGGCGTGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTCATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGCTGATTTATGAcatcat                                  <  1:342052/149‑1 (MQ=255)
                                                                                                                     ggCCTTTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGCTGATTTATGACATCATGAGTaa                            <  2:688861/149‑1 (MQ=255)
                                                                                                                     ggCCTGTATATGCTGGGGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGCGTTTCCGTGCTTATATCTACCTGACGGTTTCCGAGCTGGTGCTGTTCACCGCGCTGACGCCGCTTTACATCACGCTGATTTATGACATCATGAGTaa                            <  2:195386/149‑1 (MQ=255)
                                                                                                                      gCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGCTGATTTATGACATCATGAGTAAg                           >  2:722024/1‑149 (MQ=255)
                                                                                                                         tGTATATGCTGGTGGGCCCGATTCAGCGTGGCGTGCTGACCAGATCGAGTATCGGCGATTACAGCTGCACGGCCGGTTCCGATCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGCTGATTTATGACATCATGAGTAAgcgc                        >  2:702936/1‑149 (MQ=255)
                                                                                                                           tataTGCTGGTGGGCGCGATGCAGCTTGGCGTGCTGTATATGCTGAGTTTCCGCGCTTATCACTACCTGACGGTTTCCGAGCTGCTGCTGTGAACCGTACTGACGCCGCTCTACCTCACGCTGATTTCTGACATCATGAGTAAGCGCCg                      >  2:629441/1‑149 (MQ=255)
                                                                                                                            atatGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGATGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGCTGATTTATGACATCATGAGTAAGAGCCGt                     >  2:809798/1‑149 (MQ=255)
                                                                                                                                 cTGGTGGGCGCGATCCAGCTTGGCGTGATGTATATGATGAGTTTCCGCGCTTATCTCTCCCTGACAGTTTCCGAGAAGCTGCTGTTCACCGTGCTGACGCAGCTCTACATCACGCTGATTTATGACATCATGGGTAAGAACCGTccggc                >  2:719232/1‑145 (MQ=255)
                                                                                                                                      gggCGCGATGCAGCGTGGCGTGATTTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGCTGATTTATGACATCATGAGTAAGCGCCGTCTGCGCTggg           <  2:671209/149‑1 (MQ=255)
                                                                                                                                       ggCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACAGTGTTGGCGCCGCCCGACATCACGCTGATTTATGACATCATGAGTAAGCGCCGTCTGCGCTgggg          >  1:965172/1‑149 (MQ=255)
                                                                                                                                          gcgATGCAGCGTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGCTGATTTATGACATCATGAGTAAGCGCCGTCTGCGCTGGGGCTa       <  1:585589/149‑1 (MQ=255)
                                                                                                                                           cgATGCAGCTTGGCGTGCTGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTCTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGCTGATTTATGACCTCATGAGTAAGCGCCGTCTGCGCTGGGGCTAt      >  2:350622/1‑149 (MQ=255)
                                                                                                                                               gCAGCTTGGCGTGGTGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGATGATTTATGACATCATGAGTACTCGCCGTCTGCGCTGGGGCTATGCCt  >  2:560105/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GGCGAGTACCTTGCGGGGCACGTCGATAGCTATTTTGCGGTGCTGGTGCGCGTTGGCCTGGCGGCACTCGTTTTTCTGCCGTTTCTGCGTACCCGTGGCAATAGCCTGAAAACGGTCGGCCTGTATATGCTGGTGGGCGCGATGCAGCTTGGCGTGATGTATATGCTGAGTTTCCGCGCTTATCTCTACCTGACGGTTTCCGAGCTGCTGCTGTTCACCGTGCTGACGCCGCTCTACATCACGCTGATTTATGACATCATGAGTAAGCGCCGTCTGCGCTGGGGCTATGCCT  >  NC_000913_3_pae_tpiA/4011130‑4011421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑