Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	1,265,009	C→A	intergenic (+39/‑3)	hemA → / → prfA	glutamyl tRNA reductase/peptide chain release factor RF‑1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	1,265,009	0	C	A	100.0%	109.9 / NA	35	intergenic (+39/‑3)	hemA/prfA	glutamyl tRNA reductase/peptide chain release factor RF‑1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/16);  total (19/16)

TGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGA  >  NC_000913_3_pae_tpiA/1264864‑1265151                                                                                                                                                  |                                                                                                                                               tGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTa                                                                                                                                             >  1:386815/1‑149 (MQ=255)       aCCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGc                                                                                                                                       >  2:604454/1‑149 (MQ=255)          gCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCAGCAAGTGCCAGCGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCtt                                                                                                                                    <  1:50378/149‑1 (MQ=255)          gCTTGAGCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACACGGTGCATTTACGACTATGAAGCCtt                                                                                                                                    <  1:584680/149‑1 (MQ=255)              gATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCATCTAt                                                                                                                                >  2:482466/1‑149 (MQ=255)                tCCATGCGCCAACGATATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCg                                                                                                                              <  1:376128/149‑1 (MQ=255)                  cATGCGCAAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGtt                                                                                                                            <  1:83210/149‑1 (MQ=255)                    tGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGc                                                                                                                          <  1:604454/149‑1 (MQ=255)                          aaCGAAATCACTTCAACAGGCCGCCCGTGACGGGGCTAACGAACGCCTGAATATTCTGCGCGGCAGCCTCGGGCTGGGGTAGCAGAACATCATTTTCTTTTTTTACAGGGTGCATTTCCGACTATGAAGCCTCCTATCGTTGCCAAACt                                                                                                                    >  1:297727/1‑149 (MQ=255)                             gAAATCACTTCAACAGGCCGCCCGTGACGGGGCTAACGCACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTAACGTTGCCAAACTGGa                                                                                                                 >  1:218758/1‑149 (MQ=255)                               aaTCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTGCATCATTTTCTTGTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTGTCGTTGCCGAAATGGaaa                                                                                                               >  1:251703/1‑148 (MQ=255)                               aaTCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCGGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAg                                                                                                               <  1:283504/149‑1 (MQ=255)                                 tCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGcc                                                                                                             <  2:386815/149‑1 (MQ=255)                                                gccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCAt                                                                                              <  2:552883/149‑1 (MQ=255)                                                         ggggATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGtt                                                                                     <  1:453823/149‑1 (MQ=255)                                                         ggggATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCATTCTATCGTTGCCAAACTGGAAGGCCTGAATGAACGCCATTAAGTAGtt                                                                                     >  2:485550/1‑149 (MQ=255)                                                             aTAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAgg                                                                                 >  1:236281/1‑149 (MQ=255)                                                              tAACGAAGGCCGGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGc                                                                                <  2:443068/149‑1 (MQ=255)                                                                    acgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAGACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAg                                                                                  >  1:119628/1‑141 (MQ=255)                                                                    acgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAg                                                                                  <  2:119628/141‑1 (MQ=255)                                                                     tgCCTGAATAGTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCACTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTg                                                                         <  2:604083/148‑1 (MQ=255)                                                                          gAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTATTAATGAACATGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGa                                                                    >  1:448630/1‑149 (MQ=255)                                                                                 cTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCaa                                                             >  1:530300/1‑149 (MQ=255)                                                                                       gACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCACTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATc                                                       <  1:387494/149‑1 (MQ=255)                                                                                        aCAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCg                                                      <  1:355012/149‑1 (MQ=255)                                                                                            ccTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGa                                                  >  1:78433/1‑149 (MQ=255)                                                                                                           gCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTcg                                   >  1:461815/1‑149 (MQ=255)                                                                                                                  atcatTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGCAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTa                            >  1:181492/1‑149 (MQ=255)                                                                                                                  atcatTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCAACAACGGGGGCATGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTa                            >  1:312600/1‑149 (MQ=255)                                                                                                                    catTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATc                          <  2:181492/149‑1 (MQ=255)                                                                                                                           tttttttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATCGAGCATTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGACCGTTTTCGCACATTATCCCGCGaa                   >  2:71626/1‑149 (MQ=255)                                                                                                                                    aGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGGCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCAGCCGAAAGTGCGCAg          >  2:2340/1‑149 (MQ=255)                                                                                                                                     gagTGCATTTACGACTATGAAGTCGTCTATCGTGGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGt         <  2:251703/147‑1 (MQ=255)                                                                                                                                      ggTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGtt        >  1:362071/1‑149 (MQ=255)                                                                                                                                            tttACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGa  >  1:536743/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                               TGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGA  >  NC_000913_3_pae_tpiA/1264864‑1265151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑