Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,606,034	G→A	noncoding (48/87 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,606,034	0	G	A	100.0%	43.7 / NA	16	noncoding (48/87 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/9);  total (7/9)

CTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCT  >  NC_000913_3_pae_tpiA/4605891‑4606091                                                                                                                                                |                                                          cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                      <  2:370833/149‑1 (MQ=255)      aTCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAacac                                                 >  1:7529/1‑149 (MQ=255)           gCCTAGCACTTATTGCTGGCATCATGCTGAGTATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGaa                                            <  2:198347/149‑1 (MQ=255)                gCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCACCACGTCCGTAAGAACACTAACGCCTGAAGCTAg                                       >  2:504332/1‑149 (MQ=255)                 cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                      <  1:459846/149‑1 (MQ=255)                           tGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGGAGTAAAGATAATTGGTGCGAAGGGGGAGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGATAGCGCGTCTAcga                            >  1:197578/1‑147 (MQ=37)                                  atGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                     <  2:334684/149‑1 (MQ=255)                                      tgatgaTAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAAAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                 >  2:438504/1‑149 (MQ=255)                                      tgatgaTAAGGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                 <  1:71844/149‑1 (MQ=255)                                            tAATGAATTGGAATCAGGGATGAAAAATGAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc           <  2:247543/149‑2 (MQ=255)                                                gAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt       >  2:89733/1‑149 (MQ=255)                                                     tGAAGCAGGGATTACAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGCACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGtgcgag  <  1:480722/149‑6 (MQ=17)                                                                              agaaACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGtgc     >  1:141927/1‑119 (MQ=255)                                                                              agaaACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGtgc     <  2:141927/121‑3 (MQ=255)                                                                                       aaaGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                              >  1:26831/1‑87 (MQ=255)                                                                                       aaaGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                              <  2:26831/87‑1 (MQ=255)                                                                                                                                                |                                                          CTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCT  >  NC_000913_3_pae_tpiA/4605891‑4606091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑