New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 3335199	56 (0.940)	5 (0.090)	5/286	8.1	8.8%	intergenic (+12/+36)	sfsB/murA	malPQ operon transcriptional activator/UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
	?	NC_000913_3_pae_tpiA	= 3335219	49 (0.830)					intergenic (+32/+16)	sfsB/murA	malPQ operon transcriptional activator/UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/3335051‑3335199 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggCAGCCCCGTTCGCGGGGCTACCGCCAGACCACTCATTTTTTCG  <  NC_000913_3_pae_tpiA/3335219‑3335177                                                                                                                                                                                                  AAGGAGAGATGATTATTGCGAAAGCCCTTGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGG                                             >  2:242867/1‑149                   CGAAAGCCCGGGGAACTGACCCCTGGGTTATCTGGGCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTAAAAAAAAAAAAAAAAATGAGTGGTCTGGCGGTAGCCCCGTTCGCGGGGC                           <  2:1021168/149‑1                       AGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGATCTATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGCAGCCCCGTTCGCGGGGCTACC                       <  1:263874/149‑1                                 ACTGACCCCGGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGTAGCCCCGTTCGCGGGGCTACCGCCAGACCAC             <  2:830906/149‑1                                                                  CATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGCAGCCCCGTTCGCGGGGCTACCGCCAGACCACTCATTTTTTCG  >  1:949540/1‑127                                                                  CATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGGCAGCCCCGTTCGCGGGGCTACCGCCAGACCACTCATTTTTTCG  <  2:949540/127‑1                                                                                                                                                                                                  AAGGAGAGATGATTATTGCGAAAGCCCTGGGAACTGACCCCTGGGTTATCTGGCCATCACGCTACCATGATCCGCAGACCCATGAGTTTATCGACAGAACGCAGTTGATGCGTAGCTACACTAAACCGAAAAAATGAGTGGTCTGGCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/3335051‑3335199 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggCAGCCCCGTTCGCGGGGCTACCGCCAGACCACTCATTTTTTCG  <  NC_000913_3_pae_tpiA/3335219‑3335177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.