New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 3525427 =50 (0.840)7 (0.130) 5/272 7.8 13.0% intergenic (+4/+162) mrcA/nudE penicillin‑binding protein 1a, murein transglycosylase and transpeptidase/adenosine nucleotide hydrolase; Ap3A/Ap2A/ADP‑ribose/NADH hydrolase
?NC_000913_3_pae_tpiA 3525449 = NA (NA)intergenic (+26/+140) mrcA/nudE penicillin‑binding protein 1a, murein transglycosylase and transpeptidase/adenosine nucleotide hydrolase; Ap3A/Ap2A/ADP‑ribose/NADH hydrolase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GAAGTTTGCCTGATGCACAATGTTTATCAGGCTTATGGGGTTTCTGCAATTTATTGAGTTTTCACGATTTTGTTAGCCGGATAACGCGTTCACGCCGTATCCGGCATAAACAAGTGCACTTTGTCAGCAAACTGAAAAGGCGCCGAAGCGCCTTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/3525582‑3525427
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgccttttCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATT  >  NC_000913_3_pae_tpiA/3525449‑3525589
                                                                                                                                                                                                                                                                                                         
GAAGTTTGCCTGATGCACAATGTTTATCAGGCTTATGGGGTTTCTGCAATTTATTGAGTTTTCACGATTTTGTTAGCCGGATAACGCGTTCACGCCGTATCCGGCATACACAAGTGCACTTTGTCAGCACACTGCAAAGGCGCCCAAGC                                                                                                                                                      <  2:775354/149‑1
 AAGTTTGCCTGATGCACAATGTTTATCAGGCTTATGGGGTTTCTGCAATTTATTGAGTTTTCACGATTTTGTTAGCCGGATAACGCGTTCACGCCGTATCCGGCATAAACAAGTGCACTTTGTCAGCAAACTGAAAAGGCGCCGAAGCG                                                                                                                                                     >  2:399398/1‑149
   GTTTGCCTGATGCACAATGTTTATCAGGCTTATGGGGTTTCTGCAATTTATTGAGTTTTCACGAGTTTGTTAGCCGGATAACGCGTTCACGCCGTATCCGGCATAAACAAGTGCACTTTGTCAGCAAACTGAAAAGGCGCCGAAGAGCC                                                                                                                                                   >  1:34009/1‑149
                                                                                                 TATCCGGCATAAACAAGTGCACTTTGTCAGCAAACTGAAAAGGCGCCGAAGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGC                                                      >  1:636914/1‑148
                                                                                                 TATCCGGCATAAACAAGTGCACTTTGTCAGCAAACTGAAAAGGCGCCGAAGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGC                                                      <  2:636914/148‑1
                                                                                                                                    TGAAAAGGCGCCGAAGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGCCGTGAACGAATTATCCGGCTAACAAAATCGTAAAAACTCAATAAATTGCAGAAACCCAATAAGCCTGATAAACCTTGTGCATCAG                  >  1:405366/1‑149
                                                                                                                                       AAAGGCGCCGAAGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTAAAAACTAAATAAATTGCAGAAACACCATAAGCATGATAAACATTGTGCATCAGGCA               >  1:342610/1‑149
                                                                                                                                         AGGCGCCGAAGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAA             >  1:480020/1‑149
                                                                                                                                         AGGCGCCGAAGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAA             >  1:480064/1‑149
                                                                                                                                         AGGCGCCGAAGCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGCGAAAACTCCATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCCACACGCAAA             >  1:480012/1‑149
                                                                                                                                                tctGCGCCTTTTCAGGTTGCTGACAAAGTGTACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACG      <  1:76068/146‑1
                                                                                                                                                   GCGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTCTGCGGCTAACAAAATCGTGAAAACTCAATAAATTACGGAAACACCATAAACATGATAAACATTGTGCATCAGGCAAACTGCACGCAT   >  1:611606/1‑149
                                                                                                                                                    ctCCTTTTCAGTTTGCTCACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGTCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATT  <  2:110908/147‑1
                                                                                                                                                    CGCCTTTTCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATT  <  2:61710/149‑1
                                                                                                                                                                                                                                                                                                         
GAAGTTTGCCTGATGCACAATGTTTATCAGGCTTATGGGGTTTCTGCAATTTATTGAGTTTTCACGATTTTGTTAGCCGGATAACGCGTTCACGCCGTATCCGGCATAAACAAGTGCACTTTGTCAGCAAACTGAAAAGGCGCCGAAGCGCCTTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/3525582‑3525427
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgccttttCAGTTTGCTGACAAAGTGCACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATT  >  NC_000913_3_pae_tpiA/3525449‑3525589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.