Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,037,2271.T25.0% 7.7 / 14.1 16intergenic (+10/‑33)ileT/alaTtRNA‑Ile/tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base . (6/6);  new base T (0/4);  total (6/10)
Fisher's exact test for biased strand distribution p-value = 2.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.23e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAG  >  NC_000913_3_pae_tpiA/4037079‑4037241
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agaagCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACg                 <  1:143943/149‑1 (MQ=34)
     cGTTCTTTGAAGTGCTCACACAGATTGTCGGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCaaa           <  2:130672/149‑1 (MQ=21)
        tCTTTGAAGTGCTCACACAGATTGTCTTATGAAAATGAGCAGTAAAGCCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAAttt        <  1:243187/149‑1 (MQ=17)
         cTTTGAGGTGCTAACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCCTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCCGTGCAAATTTg       <  1:990274/149‑1 (MQ=11)
         cTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCCGTGCAAATTTg       <  1:617716/149‑1 (MQ=12)
         cTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCCGTGCAAATTTg       <  2:14662/149‑1 (MQ=255)
         cTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGATTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAGGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCCCGGCAAATTTg       >  2:291221/1‑149 (MQ=12)
         cTTTGAAGTGCTCACACAGATTGCCTGATTAAAATCAGCAGTAAAACCTTGACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTg       <  2:372901/149‑1 (MQ=11)
          tttGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGa      >  1:621929/1‑149 (MQ=32)
          tttGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCAACTCAGGCCTACCAAATTTGCACGGCAAATTTGa      >  1:907299/1‑149 (MQ=17)
          tttGAAGTGCTCACACAGATAGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGCGGTAGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATATGa      >  2:995452/1‑149 (MQ=255)
           tttaaGTGATCACACAGTTTGTCTGATGAAAATGAACAGTAAACCCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCCAGTCCACTCAGGCCTACCAAATTTGCACGGCAACATTGaa     <  2:13998/146‑1 (MQ=11)
           ttGAAGTGCTCACACAGATTGTCTGATGAAAATTAGCAGAAAAACCTCTACAGGCTTGTAGCTCCGGAGGTTAGAGCGCACCCCTGATACGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCCGATTGGaa     >  2:835097/1‑149 (MQ=11)
           ttGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGaa     <  1:18523/149‑1 (MQ=32)
            tGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCCGTGCAAATTTggta    <  2:942052/149‑4 (MQ=255)
             gAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACCGGCTTGTAGCTCAGGTGATTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCCCGGCACAttgcaggt   >  2:726747/1‑143 (MQ=11)
              aaGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTTAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAagag  <  1:373728/149‑1 (MQ=11)
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AGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAG  >  NC_000913_3_pae_tpiA/4037079‑4037241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: