Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,295,9760CT25.0% 119.9 / 50.7 92intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (45/24);  new base T (10/13);  total (55/37)
Fisher's exact test for biased strand distribution p-value = 8.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-02
Rejected as consensus: Frequency below/above cutoff threshold.

GTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGG  >  NC_000913_3_pae_tpiA/4295828‑4296121
                                                                                                                                                    |                                                                                                                                                 
gTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCGGACATCAAc                                                                                                                                                   >  2:359590/1‑149 (MQ=17)
  cGGGTAAGGGGTTTAGGCCGGGTCCGGCGTCAACGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc                                                                                                                                                 <  2:248979/149‑1 (MQ=11)
  cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc                                                                                                                                                 <  1:646634/149‑1 (MQ=32)
    gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt                                                                                                                                               >  2:340061/1‑149 (MQ=17)
    gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCt                                                                                                                                               >  1:578094/1‑149 (MQ=32)
     acacggCGTTTATGCCGCATCCGATATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGGGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg                                                                                                                                              <  1:89055/145‑1 (MQ=12)
     aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGCCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCATTAGCCTGATTTAGCGTGTTTTTGTGGGCCTGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg                                                                                                                                              >  1:454622/1‑149 (MQ=11)
       aaGGCGTTTATGCCGCATCCGACATCACCGGCTGAGGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAAGCGTTTACGCCGCATCCGACATCAGCGCCTgct                                                                                                                                            >  2:748794/1‑147 (MQ=11)
       aaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt                                                                                                                                            >  2:361027/1‑149 (MQ=17)
        aGGCGTTTATGCCGCATCCGACATCAACGGAAGGCACAACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCACTCGACATCAATGCCTGATg                                                                                                                                           >  1:775123/1‑149 (MQ=11)
         ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGGTTTAGCGTGATTTTGTAGCTCGAATGAGTCCTTTACGCCTCATCCGACATAAATCCCTGATGc                                                                                                                                          >  2:718466/1‑149 (MQ=11)
         ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCGTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCATCAATGCCTGATGc                                                                                                                                          >  1:296222/1‑149 (MQ=12)
          gCGTTTATGCCGCAGCCGACATCAGCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGCTGCg                                                                                                                                         >  1:903655/1‑149 (MQ=12)
            gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGTTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                                                                       <  1:89327/149‑1 (MQ=17)
            gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa                                                                                                                                             >  1:763839/1‑143 (MQ=32)
            gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa                                                                                                                                             <  2:763839/143‑1 (MQ=32)
            gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAc                                                                                                                                       >  1:645119/1‑149 (MQ=32)
            gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTCAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGCTGCGAc                                                                                                                                       >  2:964707/1‑149 (MQ=17)
             tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCGGTTGCggca                                                                                                                                      >  1:143565/1‑146 (MQ=11)
               tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                                                                    >  2:994698/1‑149 (MQ=32)
                 tGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTg                                                                                                                                  >  2:491670/1‑149 (MQ=32)
                   ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc                                                                                                                                >  2:482786/1‑149 (MQ=21)
                    cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGACAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGCTGCGACGCTTGCcg                                                                                                                               >  2:627693/1‑149 (MQ=255)
                     gCATCCAACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACACCAATGCCTGATGCGACGCTTGCcgc                                                                                                                              <  1:946398/149‑1 (MQ=255)
                      cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTCCcgcg                                                                                                                             >  1:823893/1‑149 (MQ=21)
                         ccGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                                                                          <  2:264527/149‑1 (MQ=255)
                           gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                        >  1:181693/1‑149 (MQ=37)
                            aCATCAACGCCTGATGCGACGTTGAACGCGTTTTATCAGGCCTACGCCAGACAGCGCAATAGCTTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCATTAATCTCTGTTGTGACGCTTGCCGCGTCTTAt                                                                                                                       <  2:1010511/149‑1 (MQ=11)
                              aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                     <  1:789253/149‑1 (MQ=21)
                               tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                    <  1:482786/149‑1 (MQ=21)
                               tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                    <  1:988973/149‑1 (MQ=21)
                               tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGGCGCTTGCCGCGTCTTATCAg                                                                                                                    >  2:138839/1‑149 (MQ=37)
                                 aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGGTTTAGCGTGATTTTGTAGATCGGATAAGACGATTACGCCGCATCCGACATCAACCACTGATGCGACACTTGCCGCGTCTTATCAgcc                                                                                                                  >  1:662764/1‑147 (MQ=11)
                                    gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                                                               >  1:785181/1‑149 (MQ=21)
                                      cTGGTGCGACGCTTTACGCGGCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGACTTAGCGTTATTTTCTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc                                                                                                             <  2:664498/149‑3 (MQ=11)
                                      cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc                                                                                                             >  2:141720/1‑147 (MQ=18)
                                      cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACg                                                                                                             >  1:663082/1‑149 (MQ=38)
                                      cTGATGCGACGATTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTACg                                                                                                             >  1:639334/1‑149 (MQ=255)
                                       tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGCTTTTGTAGGTCGGATAATGCGTTTACGCCGCATCCGACATCAACGCCCGATGCGACTCTTTCGGCGGCTTATCAGCCCTACGc                                                                                                            >  1:1022291/1‑149 (MQ=11)
                                         aTGCGACGCTTAATGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCa                                                                                                          <  1:106574/149‑1 (MQ=255)
                                             gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTAATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATAATCCCTACGCCaagcg                                                                                                      >  2:23686/1‑145 (MQ=11)
                                                gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCCCATCCGGCATCAACGCCTGATGCGACGCTTACCGCGTCTTATCCGGCCTACGCCGAACAgcg                                                                                                   >  1:53939/1‑149 (MQ=11)
                                                gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgag                                                                                                   >  2:754610/1‑147 (MQ=255)
                                                  ttAACGCGTCTTATCAGGCCTACGCCAGACAGCCCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCa                                                                                                 >  2:990363/1‑149 (MQ=255)
                                                    aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAt                                                                                               <  2:181693/149‑1 (MQ=38)
                                                     aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa                                                                                              <  1:994698/149‑1 (MQ=39)
                                                       gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc                                                                                                                                                 <  1:985591/96‑1 (MQ=21)
                                                        cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTTTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc                                                                                           >  1:689694/1‑149 (MQ=255)
                                                         ggcTTATGAGGGTTACGGCAGGCACCGCAATAGCATGATTTAGCGTGATTTTGTAGGTCGGACAAGGGGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCt                                                                                          <  2:470038/147‑1 (MQ=11)
                                                              aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa                                                                                                                                             <  1:232448/93‑1 (MQ=21)
                                                              aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa                                                                                                                                             >  2:232448/1‑93 (MQ=21)
                                                              aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCCGACAGCGCAATAGCCTGAttt                                                                                     >  1:798615/1‑149 (MQ=255)
                                                               tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa                                                                                    >  1:658854/1‑149 (MQ=38)
                                                               tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACACCAACGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCACCGCAATAGCCTGATTTa                                                                                    >  2:687000/1‑149 (MQ=255)
                                                                 aGGCCTACGCCAGAAGGCGAAATGGCCTGATTTAGCGTCATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCTTACGCCAGACAGCGCAATAGCCTGATTTAGc                                                                                  <  2:326328/149‑1 (MQ=11)
                                                                  ggaatACGCCAGACATCGAAATGGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCg                                                                                 <  2:724414/145‑1 (MQ=255)
                                                                     cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGGGTGa                                                                              >  1:171402/1‑149 (MQ=38)
                                                                     cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGa                                                                              >  1:469028/1‑149 (MQ=38)
                                                                      tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa                                                                                                                                             <  1:145908/85‑1 (MQ=25)
                                                                      tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa                                                                                                                                             >  2:145908/1‑85 (MQ=33)
                                                                      tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTATCGTCAt                                                                             >  2:572463/1‑149 (MQ=255)
                                                                         gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATATCCTGATTTAGCGTGAtttt                                                                          >  2:627617/1‑149 (MQ=38)
                                                                         gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAACCAGCGCAATATCCTGATTTAGCGTGAtttt                                                                          >  2:502016/1‑149 (MQ=255)
                                                                                 gcacAATAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCg                                                                  <  2:199887/146‑1 (MQ=255)
                                                                                    cAATAGGCTGACTTAGCGTGATTTTGTAGGGCTGGTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAt                                                               <  2:937702/149‑1 (MQ=11)
                                                                                    cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAt                                                               <  1:359590/149‑1 (MQ=37)
                                                                                       tcgCCAGATTTAGCGTTATTTTGTAGGGCGGATAAGGCGTTTACGCCGGATACGAGATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAg                                                            <  2:138397/147‑1 (MQ=11)
                                                                                          tcagATTTAGCGTCATTTTGTAAGTCGGATAAGGCGTTTACGCCGTATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCg                                                         <  2:656304/146‑1 (MQ=255)
                                                                                           cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGt                                                        >  2:787858/1‑149 (MQ=37)
                                                                                              aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCCCAGACGGCGCCTTACGCTGCATTCGCTTGATACTGTTGGTCAGACAAGGccattc                                                     >  2:618166/1‑144 (MQ=11)
                                                                                              aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGGAATAGCGTGATTTAGCGTGCTTTTGTAGGTCGACTAAGGCGTTTa                                                     >  2:337022/1‑149 (MQ=11)
                                                                                              aTTGAGCGTGATTTTGTTGGTCGGATACGGCGTTTACGCCGCGTCGGAAATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTa                                                     <  1:659987/149‑1 (MQ=11)
                                                                                                 tAGCGTGATTTTGTAGGACGGACACGGTGTTTACGCCGCACCCGACATCAACGCTTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTTATGc                                                  <  2:883109/149‑1 (MQ=11)
                                                                                                  cggtcgATTTTGTAGATCGGATAAGGCGTCTACGCCTCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGACAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc                                                 <  2:265030/144‑1 (MQ=11)
                                                                                                      tGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCtt                                                                                                                                   <  1:212875/63‑1 (MQ=255)
                                                                                                        aTTTTTTAGGTCGGATAAGGCGTTTACGCCGCATACTACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATcc                                           <  1:801347/149‑1 (MQ=255)
                                                                                                                 gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaat                                  >  1:264527/1‑148 (MQ=25)
                                                                                                                     gATAAGACGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCATGCCGCGTCTTATCAGGCCTACGCCAAACAGGGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAAGCGTTTATGCCGCATCCCACATCCACGCCt                              >  2:98527/1‑149 (MQ=11)
                                                                                                                          ggCGTTTACGCCGCATCCGACATCAACGCCTTATGCGACGCTTGCCGCGTCTTATCAGGCCTGCGCCAGACAGCGCAATAGCCTGATTTCGCGTTATTTTGTATGTTGGTTGAGGCGTTTATGCCGCTTCCGACATCAATGCCTGATGc                         >  2:787210/1‑149 (MQ=11)
                                                                                                                          ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATATACTGATTTCGCGTAATTTTGTAGGTCGGATGAGGCGTTTATGCCTGATCCGACATCAATACCTGTTGc                         >  2:787223/1‑149 (MQ=11)
                                                                                                                           gcttttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc                                                 >  2:45201/4‑124 (MQ=255)
                                                                                                                           gcttttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc                                                 <  1:45201/121‑1 (MQ=255)
                                                                                                                           gCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCg                        >  1:667684/1‑149 (MQ=39)
                                                                                                                            cGTTTACGCCGCCGCCGATGTGAACGCCTGAGGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGa                       <  2:667449/149‑1 (MQ=11)
                                                                                                                                    ccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTa                                                     <  1:772149/111‑1 (MQ=35)
                                                                                                                                         tCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTc          >  1:668418/1‑149 (MQ=21)
                                                                                                                                         tCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTc          >  2:89327/1‑149 (MQ=34)
                                                                                                                                            gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTa       >  1:573718/1‑149 (MQ=35)
                                                                                                                                               aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                                                                       <  2:985347/82‑1 (MQ=38)
                                                                                                                                               aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTGGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAATGCCTGATGCGACGCTTCAAGCGTCTTTTCa    >  2:758744/1‑149 (MQ=11)
                                                                                                                                                tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAg   <  2:667684/149‑1 (MQ=33)
                                                                                                                                                 cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAgg  <  2:469028/149‑1 (MQ=32)
                                                                                                                                                 cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACACGTCTTATCacg  >  2:1040622/1‑147 (MQ=17)
                                                                                                                                                    |                                                                                                                                                 
GTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGG  >  NC_000913_3_pae_tpiA/4295828‑4296121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: