Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,110,747 G→T intergenic (+62/+784) cdh → / ← yiiQ CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,110,7470GT98.3% 335.8 / ‑5.8 116intergenic (+62/+784)cdh/yiiQCDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (66/47);  total (67/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

GGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGT  >  NC_000913_3_pae_tpiA/4110604‑4110892
                                                                                                                                               |                                                                                                                                                 
ggCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGcccc                                                                                                                                              >  2:599968/1‑149 (MQ=255)
      acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGGAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCcgcg                                                                                                                                        >  1:989673/1‑149 (MQ=255)
      acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGCTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCcgcg                                                                                                                                        >  1:95712/1‑149 (MQ=255)
      acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGGGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCAGCCACCGATCAGACTTAAGGATGCCCCGCcgcg                                                                                                                                        >  1:313252/1‑149 (MQ=255)
       caACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGa                                                                                                                                       <  1:605123/149‑1 (MQ=255)
       caACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGa                                                                                                                                       <  1:564657/149‑1 (MQ=255)
         aCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGGGAGATTTTGCGTTAAGGCGAAGATTTAAGCAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAAACTTAAGGCTGCCCGCCGCGAGCg                                                                                                                                    >  1:601242/1‑149 (MQ=255)
           gAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAAGCGAAAAATTAAGGAAAGAAAGTGCCGGATATAAAATCAGGCACCAGTCAGACTTAAGGCTGCCCCGAcgcgcacgg                                                                                                                                   >  1:298163/1‑144 (MQ=255)
            aagtcgaCTTACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGAGAATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATTCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGt                                                                                                                                  <  2:312476/143‑1 (MQ=255)
            aaaTCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGAGTTAAGGCTGCCCCGCCGCGAGCGGt                                                                                                                                  >  1:1028203/1‑149 (MQ=255)
              aTCTACTGATCCTTAACCGTGCTTCAGCCGATGAAATTTAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTCACTCACCGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGttt                                                                                                                                <  1:202568/149‑1 (MQ=255)
               tCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTc                                                                                                                               >  1:695722/1‑149 (MQ=255)
               tCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTc                                                                                                                               >  1:572743/1‑149 (MQ=255)
                 tACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGACGAGCTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGc                                                                                                                             >  2:935035/1‑149 (MQ=255)
                   cTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCa                                                                                                                           >  1:627871/1‑149 (MQ=255)
                    tGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGTGGTTTCGCCaa                                                                                                                          >  1:495093/1‑149 (MQ=255)
                     gACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAAt                                                                                                                         >  1:724796/1‑149 (MQ=255)
                      acccTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAAtt                                                                                                                        <  1:419579/146‑1 (MQ=255)
                      aCGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCaaat                                                                                                                        >  1:742727/1‑147 (MQ=255)
                          ttAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTaa                                                                                                                    <  2:674539/149‑1 (MQ=255)
                                   cTTCAGCCGAGGAAATTCAGAATCAGCAGTGTGAGCAGTGCTGGTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGactttaga                                                                                                           >  2:962089/1‑142 (MQ=255)
                                    ttCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGa                                                                                                          >  1:484982/1‑149 (MQ=255)
                                       aGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTACGatat                                                                                                       >  1:973969/1‑149 (MQ=255)
                                        gCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCTGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATg                                                                                                      <  1:949948/149‑1 (MQ=255)
                                        gCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAATGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCAAATTCTAAGATCTTAAGATATg                                                                                                      >  1:619359/1‑149 (MQ=255)
                                         ccGAGGAAATTCAGGATCATCAGTGTGAGCTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATgg                                                                                                     <  2:398674/149‑1 (MQ=255)
                                           gAGGAAATTCAGGATCATCAGTGTGAGCTTTTGCGATAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCAGCGAGCGGTATCGCCAATTCTACGATCTTAAGCTATGGGt                                                                                                   >  1:619810/1‑149 (MQ=255)
                                           gAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAGATCCGGCCCCTGTCAGACTTAAGTCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGAACATAAGATATGGGt                                                                                                   >  1:369835/1‑149 (MQ=255)
                                           gAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGt                                                                                                   >  2:90351/1‑149 (MQ=255)
                                           gAGGAAATTCAGGACCATCAGTGTGAGATCTTGCGTTAAGGCGAAAAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCAGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATCCTAAGAACTTAAGATATGGGt                                                                                                   >  2:947176/1‑149 (MQ=255)
                                            aGGAAATTCAGGATCATCAGTGTGAGATTTAGCGTTAAGACGAAGAGTTAAGGCAAGTACGTCAGGGATGTGATAGGCGGCGCGTGGCAGACTGCAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTACGCTCCTAAGCTATggggc                                                                                                  >  2:171/1‑147 (MQ=255)
                                                aaTTCAGGATTAGCAGACGGAGACTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATAACCTCCTGCACCCGGCAATCGTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCtttt                                                                                              <  1:769205/149‑1 (MQ=255)
                                                aaTTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCCATTCTAAGATCTTAAGATATGGGTCtttt                                                                                              >  1:968438/1‑149 (MQ=255)
                                                aaTTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCtttt                                                                                              <  1:889190/149‑1 (MQ=255)
                                                aaTTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCtttc                                                                                              <  1:1008344/149‑2 (MQ=255)
                                                 aTTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATg                                                                                                      >  1:1003466/1‑140 (MQ=255)
                                                 aTTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATg                                                                                                      <  2:1003466/140‑1 (MQ=255)
                                                       gATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCa                                                                                       >  1:433807/1‑149 (MQ=255)
                                                       gATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCCGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCa                                                                                       <  1:900044/149‑1 (MQ=255)
                                                         tcatcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACg                                                                                     >  2:134488/1‑149 (MQ=255)
                                                            tcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCt                                                                                  <  2:433807/149‑1 (MQ=255)
                                                              aGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGACTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgc                                                                                >  1:93710/1‑149 (MQ=255)
                                                                tgtgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgcgc                                                                              <  2:627871/149‑1 (MQ=255)
                                                                  tgtgaTTTTGCGTTAAGGCGAATAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                            <  1:536718/146‑1 (MQ=255)
                                                                  tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCccg                                                                                                                                             >  2:860757/1‑84 (MQ=255)
                                                                  tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCccg                                                                                                                                             <  1:860757/84‑1 (MQ=255)
                                                                  tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                            >  1:1043573/1‑149 (MQ=255)
                                                                    agaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGc                                                                          <  2:132437/149‑1 (MQ=255)
                                                                     gaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGcc                                                                         >  2:764330/1‑149 (MQ=255)
                                                                         tatcGTTAAGGCGAAGAGTGAAGGAAAGTAAGTGCCGGATATGAAATCCGGTACCTGTCAGACTTAAGGTTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTGTTGCCTTCACGGCTGCGCTTGCCAGCa                                                                     <  1:788457/146‑1 (MQ=255)
                                                                             gTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGAATTAACGGCTGCGCTTTCCAGAAacac                                                                 >  1:310187/1‑149 (MQ=255)
                                                                              ttAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACt                                                                >  2:806260/1‑149 (MQ=255)
                                                                                aaGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTcc                                                              <  1:806260/149‑1 (MQ=255)
                                                                                    ggAATAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACTTGTCAGACTTAAGGCTGCCTCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTc                                                          <  1:540347/148‑1 (MQ=255)
                                                                                      aaGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGt                                                        <  2:294230/149‑1 (MQ=255)
                                                                                           ttAAGGAAAGTAAGTGCCGGGTATGAAATCCGGCACCTGCAAACCATTTTACGACCCCGCCGCCAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCCCGGCTGCGCTTGCCAGCACGACTCCCCTCGTTCCGa                                                   >  1:505884/1‑149 (MQ=255)
                                                                                           ttAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCa                                                                        >  2:704026/1‑128 (MQ=255)
                                                                                           ttAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCa                                                                        <  1:704026/128‑1 (MQ=255)
                                                                                           ttAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGa                                                   >  1:103085/1‑149 (MQ=255)
                                                                                           ttAAGGAAAGTAAGGGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGa                                                   <  2:95712/149‑1 (MQ=255)
                                                                                             aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGt                                                 >  2:78497/1‑149 (MQ=255)
                                                                                             aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGt                                                 <  2:596975/149‑1 (MQ=255)
                                                                                             aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATAGGTTTTTTGCCTTCAGGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGt                                                 >  1:602301/1‑149 (MQ=255)
                                                                                               ggAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTACCAGCAACACTCCCCTCATACCGAGttt                                               >  1:477941/1‑149 (MQ=255)
                                                                                                gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGTCGCGAGCGGATTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTa                                              >  1:479988/1‑149 (MQ=255)
                                                                                                 aaaGTAAGTGCCGGATATGAAATCAGGCACCTGTCAGACTTAAGGCTGCTCTGCCGCGAGCGGTTTCGCCAATTCTAATATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTaa                                             <  2:726165/149‑1 (MQ=255)
                                                                                                     taagtGCCGGATATGAAATCCGGCATCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAgcgc                                         <  1:631897/149‑1 (MQ=255)
                                                                                                     taaggGACGGATAGGAAATCCGGCAGCTGTCGTACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGCCATGGGTCTTTTGCCGTCACGGCTGTGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAgcgc                                         <  2:328416/149‑1 (MQ=255)
                                                                                                      aagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGcgcg                                        >  1:834912/1‑149 (MQ=255)
                                                                                                       agtGCCCGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATAGGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCgg                                       <  1:351960/149‑1 (MQ=255)
                                                                                                        gtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGc                                      <  1:181974/149‑1 (MQ=255)
                                                                                                         tGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCTTGCCGAGTTTAAGCGCGGCt                                     >  1:190670/1‑149 (MQ=255)
                                                                                                              gATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGATCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCTGCTTCAAc                                <  2:714973/149‑1 (MQ=255)
                                                                                                              gATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                                <  2:762072/149‑1 (MQ=255)
                                                                                                              gATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                                <  1:876628/149‑1 (MQ=255)
                                                                                                                    aaaTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctc                          >  1:734249/1‑149 (MQ=255)
                                                                                                                     aaTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAATCGCGGCTTCAACGTCctct                         >  1:528090/1‑149 (MQ=255)
                                                                                                                     aaTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctct                         >  2:276053/1‑149 (MQ=255)
                                                                                                                      aTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCtctc                        >  2:415753/1‑149 (MQ=255)
                                                                                                                       tCCGGCACCTGTCATACTTAAGGCTGCCCCGCCGCTAGCGGTTTTGCCAATTCTAAGATCTTAAGATATGTGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTcc                       <  1:208663/149‑1 (MQ=255)
                                                                                                                          ggCACCTGTCTGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGct                    <  1:753881/149‑1 (MQ=255)
                                                                                                                          ggCACCTGTCAGACTTAAGCCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACGCTCCCCTCCTTCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGct                    <  1:508816/149‑1 (MQ=255)
                                                                                                                            cACCTGTCAGACCTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctct                  <  2:285606/149‑1 (MQ=255)
                                                                                                                             aCCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCt                                                                                          >  1:329035/1‑76 (MQ=255)
                                                                                                                             aCCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCt                                                                                          <  2:329035/76‑1 (MQ=255)
                                                                                                                              ccTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACAGCTGCGCTTGCCAGAAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCa                >  1:612882/1‑149 (MQ=255)
                                                                                                                                 gTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACt                                                                >  1:793167/1‑98 (MQ=255)
                                                                                                                                 gTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACt                                                                <  2:793167/98‑1 (MQ=255)
                                                                                                                                 gTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACATCCTCTCCGCTCTCAATa             >  1:188870/1‑149 (MQ=255)
                                                                                                                                  tCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTGAGATCTTAAGATATGGGTCTTTTGCGTTCACGGCTGCGCTTGCCAGCAACACTCCCCACGTGCCGCGTTTAAGCGCGGCTTAAACGACCTATCCGCTCTCAATAc            >  1:793625/1‑149 (MQ=255)
                                                                                                                                  tCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTGAGATATGGGTGTTTTGCCTTCACGGCTGCGCTTGCCAGCAACGCTCGCCTCGTGCCGAGTTTAAGGTCGGCTTCAACGTCCTCTCAGCTCTCAATAc            >  2:790809/1‑149 (MQ=255)
                                                                                                                                  tCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGa                                                   >  1:1042656/1‑110 (MQ=255)
                                                                                                                                    aGACTGAAGGCTGCCCCGCCGCGAGCGGTTTCGCCATTTCTAAGATCTTAAGATATGAGTCTTTTGCCTTCCCGGCTGCGCTTGCCCGCAACACTCCCCTCGTGCCGACTTTTAGCGCGGCTTCACCGTCCTCTCCGCTCTCGATAccc          >  2:591339/1‑149 (MQ=255)
                                                                                                                                        ttAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCt                                                                                  >  2:178227/1‑73 (MQ=255)
                                                                                                                                        ttAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCt                                                                                  <  1:178227/73‑1 (MQ=255)
                                                                                                                                        ttAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGccc      >  2:727653/1‑149 (MQ=255)
                                                                                                                                        ttAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGAGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCACAGTCCTCTCCGCTCTCAATACCCGgcc      >  2:370795/1‑149 (MQ=255)
                                                                                                                                           aGGCTGTCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAg   <  2:814256/149‑1 (MQ=255)
                                                                                                                                            ggcggcCCCGCCGCGGGCGGTGTAGCCAATTCTAAAATCTTAAGATATGCGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGt  <  2:19575/149‑1 (MQ=255)
                                                                                                                                            ggcggcCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTGTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGt  <  2:455891/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGT  >  NC_000913_3_pae_tpiA/4110604‑4110892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: