Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1299499–1300697 1300697 1–1199 33 [0] [0] 33 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)32 (1.130) 31/274 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

ATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTC  >  NC_000913_3_pae_tpiA/1300557‑1300841
                                                                                                                                             |                                                                                                                                               
attactgttaggtatatggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataat                                                                                                                                          <  2:49677‑M2/8‑1 (MQ=255)
       ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGt                                                                                                                                   >  1:363098‑M2/135‑149 (MQ=255)
       ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgacattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGt                                                                                                                                   >  1:404940‑M2/135‑149 (MQ=255)
         agttataaggatttacttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgagcttgctgaagaataattttaatgatattattaattctactgcctttggtacaggaaattgctaaATAATACTCAATTGTTa                                                                                                                                 >  2:338576‑M2/133‑149 (MQ=255)
           ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                               <  2:408731‑M2/19‑1 (MQ=255)
                         ttaactgcttctcctcaccatcatgttattttcgccacatcataatgctgggcttgctgaagaataagtgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATATCGAATTGTTGAATTATTGTGCattta                                                                                                                 >  1:465538‑M2/117‑148 (MQ=255)
                             ctgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCacta                                                                                                             >  2:112517‑M2/113‑149 (MQ=255)
                               gcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactact                                                                                                           >  2:165264‑M2/111‑149 (MQ=255)
                                cttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                          <  2:473174‑M2/40‑1 (MQ=255)
                                          ctatcatgttgttttcgtccaatcattatcctgggcttggtggataagaattgaagtgatagtattaattccactgcctttggtagaggaaagtgttaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                                <  2:313360‑M2/50‑1 (MQ=255)
                                              catgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGa                                                                                            >  2:48377‑M2/96‑149 (MQ=255)
                                               atgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                           >  1:199180‑M2/95‑149 (MQ=255)
                                                   tattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGa                                                                                       <  1:425201‑M2/59‑1 (MQ=255)
                                                            cacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgcaaaATAATAATCATTTGGTAAATTAGTGTGCAGTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                              <  2:189522‑M2/68‑1 (MQ=255)
                                                                tcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAg                                                                          <  1:165264‑M2/72‑1 (MQ=255)
                                                                            gcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAtt                                                              <  2:357027‑M2/84‑1 (MQ=255)
                                                                                 ctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTATGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                         >  2:512142‑M2/61‑149 (MQ=255)
                                                                                    aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTCTGACAAAGATTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaa                                                      >  1:120559‑M2/58‑149 (MQ=255)
                                                                                      gaataattgaaatgatattattaattcaactgcatttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAACGATAGACATGCCTAGCCAATCTCTATTTgattgaata                                                    >  2:279308‑M2/56‑148 (MQ=255)
                                                                                               aaatgatattattaattccactgcctttggcgggcgggagttctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATGGAAAGAtgt                                           >  1:383525‑M2/47‑149 (MQ=255)
                                                                                                  tgctattattaattcaactgtctttgcgagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGTATTTCAGTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAGTCTCTATTTTATTGAATTGAAAGAtgtttg                                        <  2:300724‑M2/106‑1 (MQ=255)
                                                                                                   gatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTGATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAACAAtgtttgt                                       >  2:474850‑M2/43‑149 (MQ=255)
                                                                                                           taattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                               <  1:279308‑M2/115‑1 (MQ=255)
                                                                                                            aattccagtgccttgtgtagagggaagtggtaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGTAACTGTAATCAGAAAAGATAGACATGATTAGCAAATGTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATg                              <  2:159759‑M2/116‑1 (MQ=255)
                                                                                                             attccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTTCCTTTCACTACTGGAACTGTAATCAGAAAAGATAAACATGCTTAGCCAATCTCTATTTGATTAAATTGAAAGATGTTTTTTAAGGCATgg                             >  1:454411‑M2/33‑149 (MQ=255)
                                                                                                              ctccattgcctgtggtagatgaaagtgttaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTTATTGAATTGAAAGATGTTTGTTAAGGCATGGa                            <  2:155518‑M2/118‑1 (MQ=255)
                                                                                                                 cactgcctttggttgaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGc                         <  2:129644‑M2/121‑1 (MQ=255)
                                                                                                                    tgcctttggtagatgaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAGGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                      <  1:100544‑M2/124‑1 (MQ=255)
                                                                                                                        tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                  <  1:112517‑M2/128‑1 (MQ=255)
                                                                                                                             tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATTCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGTCATGGATGCAAGCTATATAtt             >  2:206694‑M2/17‑149 (MQ=255)
                                                                                                                              agaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCCCTATTTGAATTACTTGAACGATGTTCGTTAAGGCATGGATGCAAGCTATAGattt            >  1:327408‑M2/16‑148 (MQ=255)
                                                                                                                                 ggaaagtgtgaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGa         <  2:174280‑M2/137‑1 (MQ=255)
                                                                                                                                        tctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTc  <  2:363098‑M2/144‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                               
ATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTC  >  NC_000913_3_pae_tpiA/1300557‑1300841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.