Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,110,747	G→T	intergenic (+62/+784)	cdh → / ← yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,110,747	0	G	T	100.0%	106.1 / NA	36	intergenic (+62/+784)	cdh/yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/14);  total (22/14)

ACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCC  >  NC_000913_3_pae_tpiA/4110613‑4110888                                                                                                                                       |                                                                                                                                              aCGAAATCTACTGACGCTTCACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGc                                                                                                                                 >  1:75886/1‑149 (MQ=255)         tACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCGGACTTAAGGCTGCCCCGCCGCGCGCGGTTTGGc                                                                                                                         >  2:198114/1‑149 (MQ=255)           cTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCa                                                                                                                       >  1:63425/1‑149 (MQ=255)            tGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCaa                                                                                                                      >  1:56887/1‑149 (MQ=255)                 cTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCactcata                                                                                                                 >  2:128828/1‑143 (MQ=255)                   tAACCGTGCTTTAACCTAAGAAATTCAGTATCATCAGTGAAAAATTCTGGGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAg                                                                                                               <  1:299364/149‑1 (MQ=255)                   tAACCGTGATGCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGGTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAg                                                                                                               <  1:110957/149‑1 (MQ=255)                                    aGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTc                                                                                              >  2:334997/1‑149 (MQ=255)                                       aaaTTCAGGATCATGAGAGAGAGAGTTTGCGTTAAGGCGTAGAGTGAAGGAAAGGAAGTGCCGGATATGAAATCCGGCACCTGGCAGACTTAAGGCTGCCCCGGCGCGAGCGGTTTCGCCAATTCTAAGATCTGAAGATATGGGTCtgt                                                                                           <  2:106657/149‑3 (MQ=255)                                                atcttcAGGGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGCTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTccc                                                                                  >  2:396021/1‑147 (MQ=255)                                                       gtgtGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgcg                                                                           >  2:512012/1‑149 (MQ=255)                                                          tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                        >  1:259513/1‑149 (MQ=255)                                                          tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                        >  1:444332/1‑149 (MQ=255)                                                          tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                        >  2:57540/1‑149 (MQ=255)                                                              aTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTGTTGCCTTCACGGCTGCGCTTGCCa                                                                    <  1:445678/149‑1 (MQ=255)                                                                  tGCATTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGGGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCaa                                                                <  1:173778/149‑1 (MQ=255)                                                                    cGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAaca                                                              <  1:292570/149‑1 (MQ=255)                                                                      ttAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGACCTTAAGATATGGGTCTTTTGCCTTCACGGATGCGCTTGCCAGACACACt                                                            >  2:332072/1‑149 (MQ=255)                                                                         aGGCGAAGAGTTAAGGAAAGTAAGTGCTGGATATGTAATCCGGCACTTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAAATCTAAGACCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTccc                                                         <  1:68111/149‑1 (MQ=255)                                                                               agagTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAAATTTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTg                                                   <  1:203920/149‑1 (MQ=255)                                                                                   ttAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGTCAGCAACACTCCCCTCGTGCCGa                                               >  1:421597/1‑149 (MQ=255)                                                                                       ggAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACATAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGCTCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCACCACTCCCCTCGTGCAGCGttt                                           >  2:479391/1‑149 (MQ=255)                                                                                          aagtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAg                                        >  2:241381/1‑149 (MQ=255)                                                                                                  gCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACACCCCTCGTGCCGAGTTTAAGCGCGGCtt                                >  1:333984/1‑149 (MQ=255)                                                                                                     ggATATGAAATCGGGCACCTGTCAGACTTAAGGCTGCCCCACTGTGAGCGGTTTCGCCAAGTCTATGATCTTAAGATATGGGTCTTTTGCCTTAACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCGGGGTTTTAGCCCGGCTTCaa                             <  2:408652/149‑1 (MQ=255)                                                                                                      tatatGAAATCCGGCATCTTTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGCTAGGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACTCTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                            <  2:363273/148‑1 (MQ=255)                                                                                                      gATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                            <  2:494478/149‑1 (MQ=255)                                                                                                         aggAAATCCGGCACCTGTCCGACTTAAGGCTGCCCCGCCGCGAGCGTTTTCGCTAATTCTAAAATCTTATGATCGGGCGCTTGTGCCTCCACCGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTc                         <  2:44907/147‑1 (MQ=255)                                                                                                           gAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAACGCGGCTTCAACGTCct                       >  1:11617/1‑149 (MQ=255)                                                                                                           gAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTATCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTACGCGCGGCTTCCACGTccc                       >  2:247596/1‑148 (MQ=255)                                                                                                            aaaTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctc                      >  2:398093/1‑149 (MQ=255)                                                                                                                     aCCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCtctc             >  1:198386/1‑149 (MQ=255)                                                                                                                       cTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCaa           <  1:113890/149‑1 (MQ=255)                                                                                                                          tCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAc        <  2:259513/149‑1 (MQ=255)                                                                                                                            aGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAccc      >  1:16692/1‑149 (MQ=255)                                                                                                                                ttAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCCCGGCTTCAACGTCCTCTCCGCTCTCAATACcccccc  >  2:104702/1‑149 (MQ=255)                                                                                                                                       |                                                                                                                                              ACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCC  >  NC_000913_3_pae_tpiA/4110613‑4110888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑