| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 4,295,976 | 0 | C | T | 26.9% | 31.8 / 11.5 | 26 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base C (9/10); new base T (5/2); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCG > NC_000913_3_pae_tpiA/4295846‑4296111 | gCCGCATCCGACATCAACGCCAGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTACGCCGCATCCGACATCCACGCCTg > 1:213285/1‑136 (MQ=12) gtCCGACATCAACGCCTGATGCGACGGTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCg < 2:256665/135‑1 (MQ=17) aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGTCGCTTTTGACATCAACGCCTGATGCg < 1:142272/136‑1 (MQ=255) tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGa > 2:209964/1‑136 (MQ=17) cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAACGCAATAGCCTGATAAAAACAACTGTGGTGTGTCGGATAAGGCGATAACGCCGCATCCGACATAAATCCCTGATGCGACg > 1:52941/1‑136 (MQ=2) cttGATGCGACGCTTGCTGCGCCTTATAAGGCCTTCGCCAGATAGCGCAAGAGCCTGATATAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc < 2:128966/134‑1 (MQ=11) cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt > 2:136857/1‑136 (MQ=21) gatgcgatgcTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTTTGCCGCATCTGTCATCAATGCCTGATGCGACGCTTGCCGCGTCTTa < 1:32365/136‑1 (MQ=11) gATGCGACGCTGAACGCGTCGTATCAGGACTACCCCTGACGCACCAATAGCATAATTGAGGGGGATTTTGTTGGTGGTAGGAGGGGTGTTGGCCGGATGCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa < 2:217030/136‑1 (MQ=2) aTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt > 1:167766/1‑136 (MQ=21) aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGTCGTTTGCTGCGTCTTATCAGGc < 1:199041/136‑1 (MQ=255) cGCTTAACGCGTCTTATCAGGCCTACGCCAAAAAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGACTGATGCGCCGCTTGCCGCGTCTTATCAGGcc > 1:152867/1‑136 (MQ=11) ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATATAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACACCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTAc > 1:175013/1‑136 (MQ=12) aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt > 2:181526/1‑132 (MQ=9) cgTCATATCAGGCCCACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGa < 1:149668/136‑1 (MQ=255) cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGCACTACGCCAGAccg > 2:256501/1‑134 (MQ=255) ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc < 1:256501/136‑1 (MQ=38) ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc > 1:251685/1‑136 (MQ=38) aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCATCGCAATAGTCTg > 1:97103/1‑136 (MQ=255) ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg < 2:250574/58‑1 (MQ=35) gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa > 2:56445/1‑111 (MQ=255) gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa < 1:56445/111‑1 (MQ=255) tctgATCAGGCTTTTACGCCGCATCCGACATCAACGCCGGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGTCAGACAGCGTAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCa < 1:264150/133‑1 (MQ=11) cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGGAATAGCCTGATTTAGCGTGATTTTCTAGGTCGGATAAGGCGTTTATGcaccat > 2:125682/1‑131 (MQ=255) gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAAACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGAATAAGGCGTTTCTGCCGCATCCGACATCAACGCCCGACGCGAccc > 2:18831/1‑134 (MQ=11) gcATCAACCCCTGATGCGACGCTTGCCGCGTCCTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGTCGCATCCGACATCAACGCCTGATGCGACGCt < 1:254860/135‑1 (MQ=255) cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgcg > 2:19994/1‑136 (MQ=11) | GCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCG > NC_000913_3_pae_tpiA/4295846‑4296111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |