Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 2,817,170 C→A E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,817,1700CA100.0% 47.9 / NA 18E112* (GAA→TAA) yqaBfructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (7/11);  total (7/11)

CTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTA  >  NC_000913_3_pae_tpiA/2817045‑2817268
                                                                                                                             |                                                                                                  
cTGCGCGCACAACAAAAATGGGTCTGGCGCGGGTTTATGTTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGGGTAATCCCTTGTGTGTCTGCAATGCCTCTGCGATGGCGCTTTAACTCCCCGtt                                                                                          <  1:31868/136‑1 (MQ=255)
       cacaACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTACTACAg                                                                                   >  1:146773/1‑136 (MQ=255)
                                    aTGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAAACCCAGGTACGCCAGCAATGACTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTc                                                      >  2:94693/1‑136 (MQ=255)
                                                       gCAGCGACGACGGCGTTAAAACAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTTCTACAGCCATTGGGCGACGACCATGCCAACTTTTCGCCACATCAACAAGAGGaa                                   <  2:141730/136‑1 (MQ=255)
                                                            gacgacGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGt                              <  1:267462/136‑1 (MQ=255)
                                                            gacgacGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGt                              <  2:166618/136‑1 (MQ=255)
                                                             ccgacgGCGCCAAAAGTATGGCTGAAGCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCCTTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGtt                             <  2:174958/135‑1 (MQ=255)
                                                                 cgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGAc                         >  1:28886/1‑136 (MQ=255)
                                                                   gCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAGGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGc                       <  2:28886/136‑1 (MQ=255)
                                                                                   gTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGt                                                                                           >  1:220579/1‑52 (MQ=255)
                                                                                   gTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGt                                                                                           <  2:220579/52‑1 (MQ=255)
                                                                                        cccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTa  <  2:82872/136‑1 (MQ=255)
                                                                                               gcgcCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCa                                                             >  1:56712/1‑70 (MQ=255)
                                                                                               gcgcCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCa                                                             <  2:56712/70‑1 (MQ=255)
                                                                                                     gCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca                                                     >  1:171646/1‑72 (MQ=255)
                                                                                                     gCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca                                                     <  2:171646/72‑1 (MQ=255)
                                                                                                       aaTGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagc               <  2:132809/108‑1 (MQ=255)
                                                                                                       aaTGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagc               >  1:132809/1‑108 (MQ=255)
                                                                                                                             |                                                                                                  
CTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTA  >  NC_000913_3_pae_tpiA/2817045‑2817268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: