| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 264,098 | G→T | L50L (CTC→CTA) | ykfH ← | uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 264,098 | 0 | G | T | 100.0% | 20.3 / NA | 9 | L50L (CTC→CTA) | ykfH | uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (4/5); total (4/5) | |||||||||||
CCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATAT > NC_000913_3_pae_tpiA/263990‑264214 | ccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATAGCGCTAGGACTCCGGCGATATCCGGAACGGcc > 1:152789/1‑136 (MQ=255) cTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCTGGCGATATCCGGAACGGccc < 1:161413/136‑1 (MQ=255) tAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGcc < 2:110902/136‑1 (MQ=255) ttGCTCATTACAGTTCTCCACTAGTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtgt < 1:58211/136‑1 (MQ=255) cTCATTACAGTTCTCCACTATTGTCAGTTCAAGGTGATGGTCATCAGGCAGGTATCGGGCCCATTTCGGTGCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGGGATATCCGGAACGTCGCTGCCGGTGTAAt > 1:28565/1‑136 (MQ=255) aGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGTCGGTGCAGTAGTGAAAGATTCGGGa < 1:196046/136‑1 (MQ=255) tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCGGGCGATATGCGGAACGTCGGTGCCGGTGTGATGGCAGACGCTACCGTGCCCCGGATATTTGTCGGTGCGGTATGGAGAGATTCGCGACTTAGAAtt > 2:13210/1‑135 (MQ=255) cTGGCGGCGTTCGGGGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCTGTGCCGGTGTTATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTTCAGTTGCGAAAGATTCGGGACTCAGGATGCTGGCgg > 2:239950/1‑136 (MQ=17) ggcgTTCGTCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAATATTCGGGACTCAGGATGCTGGCGGGatat < 1:76014/136‑1 (MQ=255) | CCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATAT > NC_000913_3_pae_tpiA/263990‑264214 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |