Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	275,065	G→A	T18I (ACC→ATC)	insH1 ←	IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	275,065	0	G	A	100.0%	10.8 / NA	6	T18I (ACC→ATC)	insH1	IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/2);  total (4/2)

CTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTA  >  NC_000913_3_pae_tpiA/275018‑275189                                                |                                                                                                                             ctTTTCTGGTCTGACGGCACTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATCATGAACCCTGTTCTATGACTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAg                                      >  1:242935/1‑136 (MQ=255)       tGGTCTGCCGGCGCTTACTGCTGAATTCACTGTCAACGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTa                                >  1:42668/1‑136 (MQ=255)            tGACGGAGATTACTCTTGAATCTACTGTCGCCGAAAATAAGTTTAGGACTCATGATGAACCTTGTTTTATGGCTCCAGATGACAAACATGATCTCATATCATGTACTTGTTCTCACCTTCCGGAGGCGTTATGAGc                           <  1:101494/136‑1 (MQ=14)              aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTg                         >  2:177970/1‑136 (MQ=255)                                ttCACTGTCGGCGAAGATCAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTAGCACCTTCCGGAGGCGTTCTAAGCCGGCGGCCCTTTTTgcttcc       >  2:137993/1‑131 (MQ=17)                                     tGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGatta  <  1:177970/136‑1 (MQ=255)                                                |                                                                                                                             CTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTA  >  NC_000913_3_pae_tpiA/275018‑275189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑