Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	482,230	G→A	P725L (CCG→CTG)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	482,230	0	G	A	100.0%	32.8 / NA	13	P725L (CCG→CTG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/6);  total (7/6)

ACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGG  >  NC_000913_3_pae_tpiA/482101‑482337                                                                                                                                  |                                                                                                            ccacGACCGCGGTCGAGAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCTCGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTAt                                                                                                       <  2:178184/135‑1 (MQ=255)               tatatAGTCGGGCACATAGCTGCCGCCCCATGCCGCGCCCAGAGTGCTGTTAATGTCGTGGATAGAAACCCTCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGttt                                                                                         <  2:28092/135‑1 (MQ=255)                  ataGTCGTACACCTAGCTTCCGCCCCAGGCAGCCCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAGAGTGCAGGGTATCTTCCAGACCGTTTGGa                                                                                      <  2:233629/134‑1 (MQ=255)                                      cgccCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATc                                                                  >  1:107109/1‑136 (MQ=255)                                               gaGCGCCCTGAGTGGTGTTAATGTCGTTGATAGAAACACCTAGCGCCTGCGTTTTTTCCGGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTTGACGTACGCTGGTCAACATATCAGGGTGcgt                                                         <  1:111303/135‑3 (MQ=255)                                                   gcCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgct                                                     <  2:107109/136‑1 (MQ=255)                                                               gtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaagc                                         >  1:206761/1‑136 (MQ=255)                                                                         ttGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTAc                               >  1:35400/1‑136 (MQ=255)                                                                                    acCCAGCGCCTGCGCTTTTTACTTATCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTAGTTACGCGCCTGAGta                    >  1:76819/1‑135 (MQ=255)                                                                                              tGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTctt          >  2:141525/1‑134 (MQ=255)                                                                                                  cTTTTTCCTGGTCAATAACAAACTAAAACAGCAAGATATCTTCCAAACCGTTTGAACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc      >  1:8981/1‑136 (MQ=255)                                                                                                    ttttCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCGTGAGTCAGTTTTTCGTGACCaa    >  2:261401/1‑136 (MQ=255)                                                                                                      ttCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAgg  <  2:206761/136‑1 (MQ=255)                                                                                                                                  |                                                                                                            ACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGG  >  NC_000913_3_pae_tpiA/482101‑482337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑